J 2024

Cyanobacterial anatoxin-a does not induce in vitro developmental neurotoxicity, but changes gene expression patterns in co-exposure with all-trans retinoic acid

KUBÍČKOVÁ, Barbara, Šárka MARTINKOVÁ, Dáša BOHAČIAKOVÁ and Klára HILSCHEROVÁ

Basic information

Original name

Cyanobacterial anatoxin-a does not induce in vitro developmental neurotoxicity, but changes gene expression patterns in co-exposure with all-trans retinoic acid

Authors

KUBÍČKOVÁ, Barbara (203 Czech Republic, belonging to the institution), Šárka MARTINKOVÁ (203 Czech Republic, belonging to the institution), Dáša BOHAČIAKOVÁ (703 Slovakia, belonging to the institution) and Klára HILSCHEROVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Toxicology Letters, CLARE, Elsevier, 2024, 0378-4274

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30108 Toxicology

Country of publisher

Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 3.500 in 2022

Organization unit

Faculty of Science

UT WoS

001138116200001

Keywords in English

Developmental neurotoxicity; NSC; Retinoids; Differentiation; Anatoxin; Cyanotoxin

Tags

Tags

International impact, Reviewed
Změněno: 5/3/2024 15:27, Mgr. Marie Šípková, DiS.

Abstract

V originále

Cyanobacterial blooms are increasing in frequency and intensity globally, and impacting recreational waters as well as waters used for drinking water provisioning. They are sources of bioactive metabolites including retinoids and the neurotoxin anatoxin-a. Here, we investigated the effects of anatoxin-a on a differentiating in vitro human neural stem cell model previously characterised with retinoic acids. Effects on protein and gene expression upon exposure for 9 or 18 days to anatoxin-a alone or in co-exposure with all-trans retinoic acid were evaluated using a panel of neural and glial differentiation biomarkers. Anatoxin-a did not cause distinct developmental neuro-toxicity alone, or in co-exposure with retinoic acid. However, in line with its excitotoxicity, in co-exposure with 200 nM all-trans retinoic acid it reduced the differentiation of acetylcholinergic neuron subtypes in the culture at 1000 nM (highest tested concentration). While this could have substantial functional implications for the developing nervous system, there is no indication for developmental neurotoxicity beyond its (excito-)toxicity to acetylcholinergic neurons, which only occurred in co-exposure to all-trans retinoic acid.

Links

EF17_043/0009632, research and development project
Name: CETOCOEN Excellence
LM2023069, research and development project
Name: Výzkumná infrastruktura RECETOX
Investor: Ministry of Education, Youth and Sports of the CR, RECETOX research infrastructure
LX22NPO5107, research and development project
Name: Národní ústav pro neurologický výzkum
Investor: Ministry of Education, Youth and Sports of the CR, 5.1 EXCELES
722493, interní kód MU
Name: Natural Toxins and Drinking Water Quality - From Source to Tap (Acronym: NaToxAq)
Investor: European Union, MSCA Marie Skłodowska-Curie Actions (Excellent Science)
857560, interní kód MU
(CEP code: EF17_043/0009632)
Name: CETOCOEN Excellence (Acronym: CETOCOEN Excellence)
Investor: European Union, Spreading excellence and widening participation