Autoři
OLSOVSKA, Eva (203 Česká republika), Kristina CABANOVA (203 Česká republika), Oldrich MOTYKA (203 Česká republika), Hana BIELNIKOVA KRYSTOFOVA (203 Česká republika), Petra MATEJKOVA (203 Česká republika), Jirf VOVES (203 Česká republika), Vladimfr ZIDLIK (203 Česká republika), Roman MADEJA (203 Česká republika), Jiří DEMEL (203 Česká republika, domácí), Jan HALFAR (203 Česká republika) a Jana KUKUTSCHOVA (203 Česká republika)
Vydání
Environmental Toxicology and Pharmacology, Amsterdam, Elsevier Science B.V. 2023, 1382-6689
V originále
The presence of particles fixed in tissue samples due to implant degradation or disintegration plays an important role in post-operative complications. The ability to determine the size, shape, chemical composition and, above all, the number of these particles can be used in many areas of medicine. This study presents a novel, simple metal-based particle detection method using scanning electron microscopy with energy dispersive spectrometer (SEM-EDS). The presence of metal particles in biopsy specimens from long bone nail-fixated implants (10 patients with titanium steel nails and 10 patients with stainless steel nails) was studied. The samples were analysed using automated area analysis based on image binarization and brightness to 255 grayscale. The results were supplemented with histological data and statistically analysed. The method based on the software used was found to be accurate and easy to use and, thus, appears to be very suitable for particle detection in similar samples.