Detailed Information on Publication Record
2023
Telomere Length In Human Pluripotent Stem Cells
HULÍNOVÁ, Daniela, Šimon FARKAŠ, Aleš HAMPL, Miloslava FOJTOVÁ, Irena KOUTNÁ et. al.Basic information
Original name
Telomere Length In Human Pluripotent Stem Cells
Authors
HULÍNOVÁ, Daniela (203 Czech Republic, belonging to the institution), Šimon FARKAŠ (703 Slovakia, belonging to the institution), Aleš HAMPL (203 Czech Republic, belonging to the institution), Miloslava FOJTOVÁ (203 Czech Republic, belonging to the institution) and Irena KOUTNÁ (203 Czech Republic, belonging to the institution)
Edition
ISSCR Annual meeting 2023, Boston, USA, 2023
Other information
Language
English
Type of outcome
Konferenční abstrakt
Field of Study
10605 Developmental biology
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
RIV identification code
RIV/00216224:14110/23:00134712
Organization unit
Faculty of Medicine
Keywords in English
Human Pluripotent Stem Cells; Telomere Length
Tags
International impact
Změněno: 22/4/2024 14:35, Mgr. Tereza Miškechová
Abstract
V originále
Human pluripotent stem cells (hPSC), albeit human induced pluripotent stem cells (hiPSC) or human embryonic stem cells (hESC), are a powerful tool in research and also hold great potential for clinical applications. One of their basic properties is active telomerase, which leads to telomere length maintenance or even lengthening in low passages (Zeng Sicong et al., 2014). Short telomeres can lead to early senescence in cells differentiated from hPSC. Therefore we used Southern blot analysis of terminal restriction fragments (TRFs) to test if the telomere length changes during reprogramming and culture of hiPSC in low passages. Contrary to previously published work (Yehezkel Shiran et al., 2011), we did not find any trend within the tested hiPSC lines. We next tested the possible impact of culture conditions on telomere length. We compared the telomere length of hPSC lines cultured for 15 passages in feeder-dependent and feeder-free systems. We also tested the effect of hypoxic/normoxic culture conditions. We did not prove any influence of culture conditions on the telomere length.
Links
| NU22-08-00629, research and development project |
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