Hyaluronic acid-based hydrogels with tunable mechanics improved
structural and contractile properties of cells

J 2024

Hyaluronic acid-based hydrogels with tunable mechanics improved structural and contractile properties of cells

KLIMOVIČ, Šimon, Deborah BECKEROVÁ, Jakub VĚŽNÍK, Daniil KABANOV, Karel LACINA et. al.

Basic information

Original name

Hyaluronic acid-based hydrogels with tunable mechanics improved structural and contractile properties of cells

Authors

KLIMOVIČ, Šimon, Deborah BECKEROVÁ, Jakub VĚŽNÍK, Daniil KABANOV, Karel LACINA, Šárka JELÍNKOVÁ, Jaromír GUMULEC, Vladimír ROTREKL and Jan PŘIBYL

Edition

Biomaterials Advances, Elsevier B.V. 2024, 2772-9508

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1016/j.bioadv.2024.213819

Keywords in English

Hydrogels; Tunable properties; Hyaluronic acid; AFM; Holotomography; Actin structure; Mechanotransduction; HL-1; MEA

Abstract

V originále

Extracellular matrix (ECM) regulates cellular responses through mechanotransduction. The standard approach of in vitro culturing on plastic surfaces overlooks this phenomenon, so there is a need for biocompatible materials that exhibit adjustable mechanical and structural properties, promote cell adhesion and proliferation at low cost and for use in 2D or 3D cell cultures. This study presents a new tunable hydrogel system prepared from high-molecular hyaluronic acid (HA), Bovine serum albumin (BSA), and gelatin cross-linked using EDC/NHS. Hydrogels with Young's moduli (E) ranging from subunit to units of kilopascals were prepared by gradually increasing HA and BSA concentrations. Concentrated high-molecular HA network led to stiffer hydrogel with lower cluster size and swelling capacity. Medium and oxygen diffusion capability of all hydrogels showed they are suitable for 3D cell cultures. Mechanical and structural changes of mouse embryonic fibroblasts (MEFs) on hydrogels were compared with cells on standard cultivation surfaces. Experiments showed that hydrogels have suitable mechanical and cell adhesion capabilities, resulting in structural changes of actin filaments. Lastly, applying hydrogel for a more complex HL-1 cell line revealed improved mechanical and electrophysiological contractile properties.

Links

EF18_046/0015974, research and development project

Name: Modernizace České infrastruktury pro integrativní strukturní biologii

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

LX22NPO5104, research and development project

Name: Národní institut pro výzkum metabolických a kardiovaskulárních onemocnění (Acronym: CarDia)

Investor: Ministry of Education, Youth and Sports of the CR, 5.1 EXCELES

NU22J-08-00062, research and development project

Name: Mechanická a morfologická high-throughput fenotypizace nádorových buněk během smykového napětí: prediktor migračního a invazivního potenciálu

Investor: Ministry of Health of the CR, Subprogram 2 - junior

Citovat

KLIMOVIČ, Šimon, Deborah BECKEROVÁ, Jakub VĚŽNÍK, Daniil KABANOV, Karel LACINA, Šárka JELÍNKOVÁ, Jaromír GUMULEC, Vladimír ROTREKL and Jan PŘIBYL. Hyaluronic acid-based hydrogels with tunable mechanics improved structural and contractile properties of cells. Biomaterials Advances. Elsevier B.V., 2024, vol. 159, May 2024, p. 1-12. ISSN 2772-9508. Available from: https://dx.doi.org/10.1016/j.bioadv.2024.213819.

@article{2381060,
   author = {Klimovič, Šimon and Beckerová, Deborah and Věžník, Jakub and Kabanov, Daniil and Lacina, Karel and Jelínková, Šárka and Gumulec, Jaromír and Rotrekl, Vladimír and Přibyl, Jan},
   article_number = {May 2024},
   doi = {http://dx.doi.org/10.1016/j.bioadv.2024.213819},
   keywords = {Hydrogels; Tunable properties; Hyaluronic acid; AFM; Holotomography; Actin structure; Mechanotransduction; HL-1; MEA},
   language = {eng},
   issn = {2772-9508},
   journal = {Biomaterials Advances},
   title = {Hyaluronic acid-based hydrogels with tunable mechanics improved structural and contractile properties of cells},
   url = {https://www.sciencedirect.com/science/article/pii/S2772950824000621},
   volume = {159},
   year = {2024}
}

TY  - JOUR
ID  - 2381060
AU  - Klimovič, Šimon - Beckerová, Deborah - Věžník, Jakub - Kabanov, Daniil - Lacina, Karel - Jelínková, Šárka - Gumulec, Jaromír - Rotrekl, Vladimír - Přibyl, Jan
PY  - 2024
TI  - Hyaluronic acid-based hydrogels with tunable mechanics improved structural and contractile properties of cells
JF  - Biomaterials Advances
VL  - 159
IS  - May 2024
SP  - 1-12
EP  - 1-12
PB  - Elsevier B.V.
SN  - 27729508
KW  - Hydrogels
KW  - Tunable properties
KW  - Hyaluronic acid
KW  - AFM
KW  - Holotomography
KW  - Actin structure
KW  - Mechanotransduction
KW  - HL-1
KW  - MEA
UR  - https://www.sciencedirect.com/science/article/pii/S2772950824000621
N2  - Extracellular matrix (ECM) regulates cellular responses through mechanotransduction. The standard approach of in vitro culturing on plastic surfaces overlooks this phenomenon, so there is a need for biocompatible materials that exhibit adjustable mechanical and structural properties, promote cell adhesion and proliferation at low cost and for use in 2D or 3D cell cultures. This study presents a new tunable hydrogel system prepared from high-molecular hyaluronic acid (HA), Bovine serum albumin (BSA), and gelatin cross-linked using EDC/NHS. Hydrogels with Young's moduli (E) ranging from subunit to units of kilopascals were prepared by gradually increasing HA and BSA concentrations. Concentrated high-molecular HA network led to stiffer hydrogel with lower cluster size and swelling capacity. Medium and oxygen diffusion capability of all hydrogels showed they are suitable for 3D cell cultures. Mechanical and structural changes of mouse embryonic fibroblasts (MEFs) on hydrogels were compared with cells on standard cultivation surfaces. Experiments showed that hydrogels have suitable mechanical and cell adhesion capabilities, resulting in structural changes of actin filaments. Lastly, applying hydrogel for a more complex HL-1 cell line revealed improved mechanical and electrophysiological contractile properties.
ER  -

KLIMOVIČ, Šimon, Deborah BECKEROVÁ, Jakub VĚŽNÍK, Daniil KABANOV, Karel LACINA, Šárka JELÍNKOVÁ, Jaromír GUMULEC, Vladimír ROTREKL and Jan PŘIBYL. Hyaluronic acid-based hydrogels with tunable mechanics improved structural and contractile properties of cells. \textit{Biomaterials Advances}. Elsevier B.V., 2024, vol.~159, May 2024, p.~1-12. ISSN~2772-9508. Available from: https://dx.doi.org/10.1016/j.bioadv.2024.213819.

Detailed Information on Publication Record