J 2024

Hyaluronic acid-based hydrogels with tunable mechanics improved structural and contractile properties of cells

KLIMOVIČ, Šimon, Deborah BECKEROVÁ, Jakub VĚŽNÍK, Daniil KABANOV, Karel LACINA et. al.

Basic information

Original name

Hyaluronic acid-based hydrogels with tunable mechanics improved structural and contractile properties of cells

Authors

KLIMOVIČ, Šimon (203 Czech Republic, belonging to the institution), Deborah BECKEROVÁ (203 Czech Republic, belonging to the institution), Jakub VĚŽNÍK (203 Czech Republic, belonging to the institution), Daniil KABANOV (643 Russian Federation, belonging to the institution), Karel LACINA (203 Czech Republic, belonging to the institution), Šárka JELÍNKOVÁ (203 Czech Republic, belonging to the institution), Jaromír GUMULEC (203 Czech Republic, belonging to the institution), Vladimír ROTREKL (203 Czech Republic, belonging to the institution) and Jan PŘIBYL (203 Czech Republic, guarantor, belonging to the institution)

Edition

Biomaterials Advances, Elsevier B.V. 2024, 2772-9508

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1016/j.bioadv.2024.213819

UT WoS

001218651500001

Keywords in English

Hydrogels; Tunable properties; Hyaluronic acid; AFM; Holotomography; Actin structure; Mechanotransduction; HL-1; MEA

Tags

14110513, 14110518

Tags

International impact, Reviewed
Změněno: 20/11/2024 11:39, Mgr. Marie Novosadová Šípková, DiS.

Abstract

V originále

Extracellular matrix (ECM) regulates cellular responses through mechanotransduction. The standard approach of in vitro culturing on plastic surfaces overlooks this phenomenon, so there is a need for biocompatible materials that exhibit adjustable mechanical and structural properties, promote cell adhesion and proliferation at low cost and for use in 2D or 3D cell cultures. This study presents a new tunable hydrogel system prepared from high-molecular hyaluronic acid (HA), Bovine serum albumin (BSA), and gelatin cross-linked using EDC/NHS. Hydrogels with Young's moduli (E) ranging from subunit to units of kilopascals were prepared by gradually increasing HA and BSA concentrations. Concentrated high-molecular HA network led to stiffer hydrogel with lower cluster size and swelling capacity. Medium and oxygen diffusion capability of all hydrogels showed they are suitable for 3D cell cultures. Mechanical and structural changes of mouse embryonic fibroblasts (MEFs) on hydrogels were compared with cells on standard cultivation surfaces. Experiments showed that hydrogels have suitable mechanical and cell adhesion capabilities, resulting in structural changes of actin filaments. Lastly, applying hydrogel for a more complex HL-1 cell line revealed improved mechanical and electrophysiological contractile properties.

Links

EF18_046/0015974, research and development project
Name: Modernizace České infrastruktury pro integrativní strukturní biologii
LM2023042, research and development project
Name: Česká infrastruktura pro integrativní strukturní biologii
Investor: Ministry of Education, Youth and Sports of the CR, CIISB - Czech Infrastructure for Integrative Structural Biology
LQ1601, research and development project
Name: CEITEC 2020 (Acronym: CEITEC2020)
Investor: Ministry of Education, Youth and Sports of the CR
LX22NPO5104, research and development project
Name: Národní institut pro výzkum metabolických a kardiovaskulárních onemocnění (Acronym: CarDia)
Investor: Ministry of Education, Youth and Sports of the CR, 5.1 EXCELES
NU22J-08-00062, research and development project
Name: Mechanická a morfologická high-throughput fenotypizace nádorových buněk během smykového napětí: prediktor migračního a invazivního potenciálu
Investor: Ministry of Health of the CR, Subprogram 2 - junior
Displayed: 23/12/2024 15:13