J 2024

Colonization dynamic and distribution of the endophytic fungus <i>Microdochium bolleyi</i> in plants measured by qPCR

MATUŠINSKY, Pavel, Vendula FLOROVÁ, Božena SEDLÁKOVÁ, Patrik MLČOCH, Dominik BLEŠA et. al.

Basic information

Original name

Colonization dynamic and distribution of the endophytic fungus <i>Microdochium bolleyi</i> in plants measured by qPCR

Authors

MATUŠINSKY, Pavel, Vendula FLOROVÁ, Božena SEDLÁKOVÁ, Patrik MLČOCH and Dominik BLEŠA (203 Czech Republic, belonging to the institution)

Edition

Plos one, Public Library of Science, 2024, 1932-6203

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

40106 Agronomy, plant breeding and plant protection;

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 3.700 in 2022

Organization unit

Faculty of Science

DOI

UT WoS

001158471300089

Keywords in English

Wheat; Seeds; Fungi; Plant tissues; Light microscopy; Leaves; Plant fungal pathogens; Polymerase chain reaction

Tags

Tags

International impact, Reviewed
Změněno: 8/3/2024 10:19, Mgr. Marie Šípková, DiS.

Abstract

V originále

Microdochium bolleyi is a fungal endophyte of cereals and grasses proposed as an ideal model organism for studying plant-endophyte interactions. A qPCR-based diagnostic assay was developed to detect M. bolleyi in wheat and Brachypodium distachyon tissues using the species-specific primers MbqITS derived from the ITS of the ribosomal gene. Specificity was tested against 20 fungal organisms associated with barley and wheat. Colonization dynamics, endophyte distribution in the plant, and potential of the seed transmission were analyzed in the wheat and model plant B. distachyon. The colonization of plants by endophyte starts from the germinating seed, where the seed coats are first strongly colonized, then the endophyte spreads to the adjacent parts, crown, roots near the crown, and basal parts of the stem. While in the lower distal parts of roots, the concentration of M. bolleyi DNA did not change significantly in successive samplings (30, 60, 90, 120, and 150 days after inoculation), there was a significant increase over time in the roots 1 cm under crown, crowns and stem bases. The endophyte reaches the higher parts of the base (2-4 cm above the crown) 90 days after sowing in wheat and 150 days in B. distachyon. The endophyte does not reach both host species' leaves, peduncles, and ears. Regarding the potential for seed transmission, endophyte was not detected in harvested grains of plants with heavily colonized roots. Plants grown from seeds derived from parental plants heavily colonized by endophyte did not exhibit any presence of the endophyte, so transmission by seeds was not confirmed. The course of colonization dynamics and distribution in the plant was similar for both hosts tested, with two differences: the base of the wheat stem was colonized earlier, but B. distachyon was occupied more intensively and abundantly than wheat. Thus, the designed species-specific primers could detect and quantify the endophyte in planta.