Markers of dental pulp stem cells in in vivo developmental
context

J 2023

Markers of dental pulp stem cells in in vivo developmental context

ŠVANDOVÁ, Eva, Barbora VESELA, Adela KRATOCHVILOVA, Kateřina HOLOMKOVÁ, Veronika ORALOVA et. al.

Basic information

Original name

Markers of dental pulp stem cells in in vivo developmental context

Authors

ŠVANDOVÁ, Eva (203 Czech Republic, belonging to the institution), Barbora VESELA (203 Czech Republic), Adela KRATOCHVILOVA (203 Czech Republic), Kateřina HOLOMKOVÁ (203 Czech Republic, belonging to the institution), Veronika ORALOVA (203 Czech Republic), Katerina DADAKOVA (203 Czech Republic), Tom BURGER (203 Czech Republic), Paul SHARPE (203 Czech Republic), Herve LESOT (203 Czech Republic) and Eva MATALOVA (203 Czech Republic)

Edition

Annals of Anatomy - Anatomischer Anzeiger, Munich, Elsevier, 2023, 0940-9602

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.200 in 2022

RIV identification code

RIV/00216224:14110/23:00134419

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1016/j.aanat.2023.152149

UT WoS

001068775700001

Keywords in English

Dental pulp; Development; Stem cell markers; In vivo

Abstract

V originále

Teeth and their associated tissues contain several populations of mesenchymal stem cells, one of which is represented by dental pulp stem cells (DPSCs). These cells have mainly been characterised in vitro and numerous positive and negati ve markers for these cells have been suggested. To investigate the presence and localization of these molecules during development, forming dental pulp was examined using the mouse first mandibular molar as a model. The stages corresponding to postnatal (P) days 0, 7, 14, and 21 were investigated. The expression was monitored using customised PCR Arrays. Additionally, in situ localization of the key trio of markers (Cd73, Cd90, Cd105 coded by genes Nt5e, Thy1, Eng) was performed at prenatal and postnatal stages using immunohistochemistry. The expression panel of 24 genes assigned as in vitro markers of DPSCs or mesenchymal stem cells (MSCs) revealed their developmental dynamics during formation of dental pulp mesenchyme. Among the positive markers, Vcam1, Fgf2, Nes were identified as increasing and Cd44, Cd59b, Mcam, Alcam as decreasing between perinatal vs. postnatal stages towards adulthood. Within the panel of negative DPSC markers, Cd14, Itgb2, Ptprc displayed increased and Cd24a decreased levels at later stages of pulp formation. Within the key trio of markers, Nt5e did not show any significant expression difference within the investigated period. Thy1 displayed a strong decrease between P0 and P7 while Eng increased between these stages. In situ localization of Cd73, Cd90 and Cd105 showed them overlap in differentiated odontoblasts and in the sub-odontoblastic layer that is speculated to host odontoblast progenitors. The highly prevalent expression of particularly Cd73 and Cd90 opens the question of potential multiple functions of these molecules. The results from this study add to the in vitro based knowledge by showing dynamics in the expression of DPSC/MSC markers during dental pulp formation in an in vivo context and thus with respect to the natural environment important for commitment of stem cells. & COPY; 2023 Published by Elsevier GmbH.

Links

GA21-21409S, research and development project

Name: Fyziologické vlastnosti a funkce kmenových buněk vztahujících se k dentici se zaměřením na kontext in vivo

Investor: Czech Science Foundation

Citovat

ŠVANDOVÁ, Eva, Barbora VESELA, Adela KRATOCHVILOVA, Kateřina HOLOMKOVÁ, Veronika ORALOVA, Katerina DADAKOVA, Tom BURGER, Paul SHARPE, Herve LESOT and Eva MATALOVA. Markers of dental pulp stem cells in in vivo developmental context. Annals of Anatomy - Anatomischer Anzeiger. Munich: Elsevier, 2023, vol. 250, October 2023, p. 1-10. ISSN 0940-9602. Available from: https://dx.doi.org/10.1016/j.aanat.2023.152149.

@article{2382297,
   author = {Švandová, Eva and Vesela, Barbora and Kratochvilova, Adela and Holomková, Kateřina and Oralova, Veronika and Dadakova, Katerina and Burger, Tom and Sharpe, Paul and Lesot, Herve and Matalova, Eva},
   article_location = {Munich},
   article_number = {October 2023},
   doi = {http://dx.doi.org/10.1016/j.aanat.2023.152149},
   keywords = {Dental pulp; Development; Stem cell markers; In vivo},
   language = {eng},
   issn = {0940-9602},
   journal = {Annals of Anatomy - Anatomischer Anzeiger},
   title = {Markers of dental pulp stem cells in in vivo developmental context},
   url = {https://www.sciencedirect.com/science/article/pii/S0940960223001048?via%3Dihub},
   volume = {250},
   year = {2023}
}

TY  - JOUR
ID  - 2382297
AU  - Švandová, Eva - Vesela, Barbora - Kratochvilova, Adela - Holomková, Kateřina - Oralova, Veronika - Dadakova, Katerina - Burger, Tom - Sharpe, Paul - Lesot, Herve - Matalova, Eva
PY  - 2023
TI  - Markers of dental pulp stem cells in in vivo developmental context
JF  - Annals of Anatomy - Anatomischer Anzeiger
VL  - 250
IS  - October 2023
SP  - 1-10
EP  - 1-10
PB  - Elsevier
SN  - 09409602
KW  - Dental pulp
KW  - Development
KW  - Stem cell markers
KW  - In vivo
UR  - https://www.sciencedirect.com/science/article/pii/S0940960223001048?via%3Dihub
N2  - Teeth and their associated tissues contain several populations of mesenchymal stem cells, one of which is represented by dental pulp stem cells (DPSCs). These cells have mainly been characterised in vitro and numerous positive and negati ve markers for these cells have been suggested. To investigate the presence and localization of these molecules during development, forming dental pulp was examined using the mouse first mandibular molar as a model. The stages corresponding to postnatal (P) days 0, 7, 14, and 21 were investigated. The expression was monitored using customised PCR Arrays. Additionally, in situ localization of the key trio of markers (Cd73, Cd90, Cd105 coded by genes Nt5e, Thy1, Eng) was performed at prenatal and postnatal stages using immunohistochemistry. The expression panel of 24 genes assigned as in vitro markers of DPSCs or mesenchymal stem cells (MSCs) revealed their developmental dynamics during formation of dental pulp mesenchyme. Among the positive markers, Vcam1, Fgf2, Nes were identified as increasing and Cd44, Cd59b, Mcam, Alcam as decreasing between perinatal vs. postnatal stages towards adulthood. Within the panel of negative DPSC markers, Cd14, Itgb2, Ptprc displayed increased and Cd24a decreased levels at later stages of pulp formation. Within the key trio of markers, Nt5e did not show any significant expression difference within the investigated period. Thy1 displayed a strong decrease between P0 and P7 while Eng increased between these stages. In situ localization of Cd73, Cd90 and Cd105 showed them overlap in differentiated odontoblasts and in the sub-odontoblastic layer that is speculated to host odontoblast progenitors. The highly prevalent expression of particularly Cd73 and Cd90 opens the question of potential multiple functions of these molecules. The results from this study add to the in vitro based knowledge by showing dynamics in the expression of DPSC/MSC markers during dental pulp formation in an in vivo context and thus with respect to the natural environment important for commitment of stem cells. & COPY; 2023 Published by Elsevier GmbH.
ER  -

ŠVANDOVÁ, Eva, Barbora VESELA, Adela KRATOCHVILOVA, Kateřina HOLOMKOVÁ, Veronika ORALOVA, Katerina DADAKOVA, Tom BURGER, Paul SHARPE, Herve LESOT and Eva MATALOVA. Markers of dental pulp stem cells in in vivo developmental context. \textit{Annals of Anatomy - Anatomischer Anzeiger}. Munich: Elsevier, 2023, vol.~250, October 2023, p.~1-10. ISSN~0940-9602. Available from: https://dx.doi.org/10.1016/j.aanat.2023.152149.

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