Detailed Information on Publication Record
2023
Urany-Less Low Voltage Transmission Electron Microscopy: A Powerful Tool for Ultrastructural Studying of Cyanobacterial Cells
MRAZOVA, Katerina, Jaromir BACOVSKY, Zuzana SEDRLOVA, Eva SLANINOVA, Stanislav OBRUCA et. al.Basic information
Original name
Urany-Less Low Voltage Transmission Electron Microscopy: A Powerful Tool for Ultrastructural Studying of Cyanobacterial Cells
Authors
MRAZOVA, Katerina, Jaromir BACOVSKY, Zuzana SEDRLOVA, Eva SLANINOVA, Stanislav OBRUCA, Ines FRITZ and Vladislav KRZYZANEK
Edition
Microorganisms, Basel, MDPI, 2023, 2076-2607
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10606 Microbiology
Country of publisher
Switzerland
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 4.500 in 2022
RIV identification code
RIV/00216224:90242/23:00133754
UT WoS
000979573000001
Keywords in English
low voltage electron microscopy; uranyl acetate; contrasting agents; transmission electron microscopy; Synechocystis; polyhydroxyalkanoates
Tags
International impact, Reviewed
Změněno: 11/4/2024 23:18, Mgr. Michal Petr
Abstract
V originále
Sample preparation protocols for conventional high voltage transmission electron microscopy (TEM) heavily rely on the usage of staining agents containing various heavy metals, most commonly uranyl acetate and lead citrate. However high toxicity, rising legal regulations, and problematic waste disposal of uranyl acetate have increased calls for the reduction or even complete replacement of this staining agent. One of the strategies for uranyless imaging is the employment of low-voltage transmission electron microscopy. To investigate the influence of different imaging and staining strategies on the final image of cyanobacterial cells, samples stained by uranyl acetate with lead citrate, as well as unstained samples, were observed using TEM and accelerating voltages of 200 kV or 25 kV. Moreover, to examine the possibilities of reducing chromatic aberration, which often causes issues when imaging using electrons of lower energies, samples were also imaged using a scanning transmission electron microscopy at 15 kV accelerating voltages. The results of this study demonstrate that low-voltage electron microscopy offers great potential for uranyless electron microscopy.
Links
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