Urany-Less Low Voltage Transmission Electron Microscopy: A
Powerful Tool for Ultrastructural Studying of Cyanobacterial
Cells

J 2023

Urany-Less Low Voltage Transmission Electron Microscopy: A Powerful Tool for Ultrastructural Studying of Cyanobacterial Cells

MRAZOVA, Katerina, Jaromir BACOVSKY, Zuzana SEDRLOVA, Eva SLANINOVA, Stanislav OBRUCA et. al.

Basic information

Original name

Urany-Less Low Voltage Transmission Electron Microscopy: A Powerful Tool for Ultrastructural Studying of Cyanobacterial Cells

Authors

MRAZOVA, Katerina, Jaromir BACOVSKY, Zuzana SEDRLOVA, Eva SLANINOVA, Stanislav OBRUCA, Ines FRITZ and Vladislav KRZYZANEK

Edition

Microorganisms, Basel, MDPI, 2023, 2076-2607

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10606 Microbiology

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.500 in 2022

RIV identification code

RIV/00216224:90242/23:00133754

DOI

http://dx.doi.org/10.3390/microorganisms11040888

UT WoS

000979573000001

Keywords in English

low voltage electron microscopy; uranyl acetate; contrasting agents; transmission electron microscopy; Synechocystis; polyhydroxyalkanoates

Abstract

V originále

Sample preparation protocols for conventional high voltage transmission electron microscopy (TEM) heavily rely on the usage of staining agents containing various heavy metals, most commonly uranyl acetate and lead citrate. However high toxicity, rising legal regulations, and problematic waste disposal of uranyl acetate have increased calls for the reduction or even complete replacement of this staining agent. One of the strategies for uranyless imaging is the employment of low-voltage transmission electron microscopy. To investigate the influence of different imaging and staining strategies on the final image of cyanobacterial cells, samples stained by uranyl acetate with lead citrate, as well as unstained samples, were observed using TEM and accelerating voltages of 200 kV or 25 kV. Moreover, to examine the possibilities of reducing chromatic aberration, which often causes issues when imaging using electrons of lower energies, samples were also imaged using a scanning transmission electron microscopy at 15 kV accelerating voltages. The results of this study demonstrate that low-voltage electron microscopy offers great potential for uranyless electron microscopy.

Links

90242, large research infrastructures

Name: CIISB III

Citovat

MRAZOVA, Katerina, Jaromir BACOVSKY, Zuzana SEDRLOVA, Eva SLANINOVA, Stanislav OBRUCA, Ines FRITZ and Vladislav KRZYZANEK. Urany-Less Low Voltage Transmission Electron Microscopy: A Powerful Tool for Ultrastructural Studying of Cyanobacterial Cells. Microorganisms. Basel: MDPI, 2023, vol. 11, No 4, p. 1-13. ISSN 2076-2607. Available from: https://dx.doi.org/10.3390/microorganisms11040888.

@article{2383019,
   author = {Mrazova, Katerina and Bacovsky, Jaromir and Sedrlova, Zuzana and Slaninova, Eva and Obruca, Stanislav and Fritz, Ines and Krzyzanek, Vladislav},
   article_location = {Basel},
   article_number = {4},
   doi = {http://dx.doi.org/10.3390/microorganisms11040888},
   keywords = {low voltage electron microscopy; uranyl acetate; contrasting agents; transmission electron microscopy; Synechocystis; polyhydroxyalkanoates},
   language = {eng},
   issn = {2076-2607},
   journal = {Microorganisms},
   title = {Urany-Less Low Voltage Transmission Electron Microscopy: A Powerful Tool for Ultrastructural Studying of Cyanobacterial Cells},
   url = {https://www.mdpi.com/2076-2607/11/4/888},
   volume = {11},
   year = {2023}
}

TY  - JOUR
ID  - 2383019
AU  - Mrazova, Katerina - Bacovsky, Jaromir - Sedrlova, Zuzana - Slaninova, Eva - Obruca, Stanislav - Fritz, Ines - Krzyzanek, Vladislav
PY  - 2023
TI  - Urany-Less Low Voltage Transmission Electron Microscopy: A Powerful Tool for Ultrastructural Studying of Cyanobacterial Cells
JF  - Microorganisms
VL  - 11
IS  - 4
SP  - 1-13
EP  - 1-13
PB  - MDPI
SN  - 20762607
KW  - low voltage electron microscopy
KW  - uranyl acetate
KW  - contrasting agents
KW  - transmission electron microscopy
KW  - Synechocystis
KW  - polyhydroxyalkanoates
UR  - https://www.mdpi.com/2076-2607/11/4/888
N2  - Sample preparation protocols for conventional high voltage transmission electron microscopy (TEM) heavily rely on the usage of staining agents containing various heavy metals, most commonly uranyl acetate and lead citrate. However high toxicity, rising legal regulations, and problematic waste disposal of uranyl acetate have increased calls for the reduction or even complete replacement of this staining agent. One of the strategies for uranyless imaging is the employment of low-voltage transmission electron microscopy. To investigate the influence of different imaging and staining strategies on the final image of cyanobacterial cells, samples stained by uranyl acetate with lead citrate, as well as unstained samples, were observed using TEM and accelerating voltages of 200 kV or 25 kV. Moreover, to examine the possibilities of reducing chromatic aberration, which often causes issues when imaging using electrons of lower energies, samples were also imaged using a scanning transmission electron microscopy at 15 kV accelerating voltages. The results of this study demonstrate that low-voltage electron microscopy offers great potential for uranyless electron microscopy.
ER  -

MRAZOVA, Katerina, Jaromir BACOVSKY, Zuzana SEDRLOVA, Eva SLANINOVA, Stanislav OBRUCA, Ines FRITZ and Vladislav KRZYZANEK. Urany-Less Low Voltage Transmission Electron Microscopy: A Powerful Tool for Ultrastructural Studying of Cyanobacterial Cells. \textit{Microorganisms}. Basel: MDPI, 2023, vol.~11, No~4, p.~1-13. ISSN~2076-2607. Available from: https://dx.doi.org/10.3390/microorganisms11040888.

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