KOUTNA, Eliska, Vanda LUX, Tomas KOUBA, Jana SKERLOVA, Jiří NOVÁČEK, Pavel SRB, Rozalie HEXNEROVA, Hana SVACHOVA, Zdenek KUKACKA, Petr NOVAK, Milan FABRY, Simon POEPSEL and Vaclav VEVERKA. Multivalency of nucleosome recognition by LEDGF. Nucleic acids research. Oxford: Oxford University Press, 2023, vol. 51, No 18, p. 10011-10025. ISSN 0305-1048. Available from: https://dx.doi.org/10.1093/nar/gkad674. |
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@article{2383603, author = {Koutna, Eliska and Lux, Vanda and Kouba, Tomas and Skerlova, Jana and Nováček, Jiří and Srb, Pavel and Hexnerova, Rozalie and Svachova, Hana and Kukacka, Zdenek and Novak, Petr and Fabry, Milan and Poepsel, Simon and Veverka, Vaclav}, article_location = {Oxford}, article_number = {18}, doi = {http://dx.doi.org/10.1093/nar/gkad674}, keywords = {PWWP DOMAIN; RECOMBINANT HISTONES; STRUCTURAL BASIS; BINDING; REFINEMENT; ASSIGNMENT; DNA; COACTIVATOR}, language = {eng}, issn = {0305-1048}, journal = {Nucleic acids research}, title = {Multivalency of nucleosome recognition by LEDGF}, url = {https://academic.oup.com/nar/article/51/18/10011/7249925?login=true}, volume = {51}, year = {2023} }
TY - JOUR ID - 2383603 AU - Koutna, Eliska - Lux, Vanda - Kouba, Tomas - Skerlova, Jana - Nováček, Jiří - Srb, Pavel - Hexnerova, Rozalie - Svachova, Hana - Kukacka, Zdenek - Novak, Petr - Fabry, Milan - Poepsel, Simon - Veverka, Vaclav PY - 2023 TI - Multivalency of nucleosome recognition by LEDGF JF - Nucleic acids research VL - 51 IS - 18 SP - 10011-10025 EP - 10011-10025 PB - Oxford University Press SN - 03051048 KW - PWWP DOMAIN KW - RECOMBINANT HISTONES KW - STRUCTURAL BASIS KW - BINDING KW - REFINEMENT KW - ASSIGNMENT KW - DNA KW - COACTIVATOR UR - https://academic.oup.com/nar/article/51/18/10011/7249925?login=true N2 - Eukaryotic transcription is dependent on specific histone modifications. Their recognition by chromatin readers triggers complex processes relying on the coordinated association of transcription regulatory factors. Although various modification states of a particular histone residue often lead to differential outcomes, it is not entirely clear how they are discriminated. Moreover, the contribution of intrinsically disordered regions outside of the specialized reader domains to nucleosome binding remains unexplored. Here, we report the structures of a PWWP domain from transcriptional coactivator LEDGF in complex with the H3K36 di- and trimethylated nucleosome, indicating that both methylation marks are recognized by PWWP in a highly conserved manner. We identify a unique secondary interaction site for the PWWP domain at the interface between the acidic patch and nucleosomal DNA that might contribute to an H3K36-methylation independent role of LEDGF. We reveal DNA interacting motifs in the intrinsically disordered region of LEDGF that discriminate between the intra- or extranucleosomal DNA but remain dynamic in the context of dinucleosomes. The interplay between the LEDGF H3K36-methylation reader and protein binding module mediated by multivalent interactions of the intrinsically disordered linker with chromatin might help direct the elongation machinery to the vicinity of RNA polymerase II, thereby facilitating productive elongation. ER -
KOUTNA, Eliska, Vanda LUX, Tomas KOUBA, Jana SKERLOVA, Jiří NOVÁČEK, Pavel SRB, Rozalie HEXNEROVA, Hana SVACHOVA, Zdenek KUKACKA, Petr NOVAK, Milan FABRY, Simon POEPSEL and Vaclav VEVERKA. Multivalency of nucleosome recognition by LEDGF. \textit{Nucleic acids research}. Oxford: Oxford University Press, 2023, vol.~51, No~18, p.~10011-10025. ISSN~0305-1048. Available from: https://dx.doi.org/10.1093/nar/gkad674.
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