ELEMENTS AND PROTEINS IMAGING BY LA-ICP-MS: MEDICAL AND
PHARMACEUTICAL RESEARCH

p 2023

ELEMENTS AND PROTEINS IMAGING BY LA-ICP-MS: MEDICAL AND PHARMACEUTICAL RESEARCH

KUCHYNKA, Michaela, Marcela VLČNOVSKÁ, Kim KALLALA, Jan BISKUPIČ, Jana HLOŽKOVÁ et. al.

Basic information

Original name

ELEMENTS AND PROTEINS IMAGING BY LA-ICP-MS: MEDICAL AND PHARMACEUTICAL RESEARCH

Name in Czech

Zobrazování prvků a proteinů pomocí metody LA-ICP-MS: medicínský a farmaceutický výzkum

Authors

KUCHYNKA, Michaela (203 Czech Republic, guarantor, belonging to the institution), Marcela VLČNOVSKÁ (203 Czech Republic, belonging to the institution), Kim KALLALA (203 Czech Republic, belonging to the institution), Jan BISKUPIČ (203 Czech Republic, belonging to the institution), Jana HLOŽKOVÁ (203 Czech Republic, belonging to the institution), Peter SCHEER (203 Czech Republic, belonging to the institution), Tereza PADRTOVÁ (203 Czech Republic, belonging to the institution), Radka OPATŘILOVÁ (203 Czech Republic, belonging to the institution), Markéta VACULOVIČOVÁ (203 Czech Republic), Viktor KANICKÝ (203 Czech Republic, belonging to the institution), Michal MASAŘÍK (203 Czech Republic, belonging to the institution) and Tomáš VACULOVIČ (203 Czech Republic, belonging to the institution)

Edition

Vyzvaná přednáška na výzkumném institutu - Vall d'Hebron Talks by VHIR, 2023

Other information

Language

English

Type of outcome

Vyžádané přednášky

Field of Study

10406 Analytical chemistry

Country of publisher

Spain

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Vall d'Hebron Talks by VHIR 'Elements and proteins imaging by LA-ICP-MS: medical and pharmaceutical research'

RIV identification code

RIV/00216224:14160/23:00133769

Organization unit

Faculty of Pharmacy

Keywords (in Czech)

biologické zobrazování; laserová ablace; detekce proteinu; prvková distribuce

Keywords in English

Bioimaging; laser ablation; protein detection; element distribution

Abstract

V originále

In the last decades, increased interest in imaging of elements/metals/proteins distribution, mainly in pharmaceutical, biomedicine research, or life science with direct application of research to specific problem/disease [1-3]. The main emphasis of this contribution is to demonstrate the unique method of laser ablation with inductively coupled plasma and mass spectrometry (LA-ICP-MS). It is a well-established method for multi-elemental analysis of elements at trace and ultra-trace. Nowadays, it is also starting to be used as a technique for the simultaneous specific determination of the protein of interest, which opens up the possibility of achieving the so-called multiplex analysis. Protein determination, protein imaging and finally their distribution in clinical samples are presented. Proteins are imaged using an immunohistochemical method (binding nanoparticle-labeled antibodies to a specific protein) and LA-ICP-MS. The main advantages of utilizing LA-ICP-MS are the acquisition of comprehensive (Metallo)proteomic information about the tissue of a given disease (in this case, cancer or stroke) or low detection limits compared to other conventionally employed protein imaging techniques in combination with multiplex analysis of one sample.

Links

MUNI/R/1568/2020, interní kód MU

Name: Study the effectiveness of anticancer drugs (Acronym: STEAD)

Investor: Masaryk University, CAREER RESTART

Citovat

KUCHYNKA, Michaela, Marcela VLČNOVSKÁ, Kim KALLALA, Jan BISKUPIČ, Jana HLOŽKOVÁ, Peter SCHEER, Tereza PADRTOVÁ, Radka OPATŘILOVÁ, Markéta VACULOVIČOVÁ, Viktor KANICKÝ, Michal MASAŘÍK and Tomáš VACULOVIČ. ELEMENTS AND PROTEINS IMAGING BY LA-ICP-MS: MEDICAL AND PHARMACEUTICAL RESEARCH. In Vyzvaná přednáška na výzkumném institutu - Vall d'Hebron Talks by VHIR. 2023.

@misc{2383639,
   author = {Kuchynka, Michaela and Vlčnovská, Marcela and Kallala, Kim and Biskupič, Jan and Hložková, Jana and Scheer, Peter and Padrtová, Tereza and Opatřilová, Radka and Vaculovičová, Markéta and Kanický, Viktor and Masařík, Michal and Vaculovič, Tomáš},
   booktitle = {Vyzvaná přednáška na výzkumném institutu - Vall d'Hebron Talks by VHIR},
   keywords = {Bioimaging; laser ablation; protein detection; element distribution},
   language = {eng},
   title = {ELEMENTS AND PROTEINS IMAGING BY LA-ICP-MS: MEDICAL AND PHARMACEUTICAL RESEARCH},
   url = {https://www.vallhebron.com/actualitat/agenda/vall-dhebron-talks-vhir-elements-and-proteins-imaging-la-icp-ms-medical-and-pharmaceutical-research},
   year = {2023}
}

TY  - SLIDE
ID  - 2383639
AU  - Kuchynka, Michaela - Vlčnovská, Marcela - Kallala, Kim - Biskupič, Jan - Hložková, Jana - Scheer, Peter - Padrtová, Tereza - Opatřilová, Radka - Vaculovičová, Markéta - Kanický, Viktor - Masařík, Michal - Vaculovič, Tomáš
PY  - 2023
TI  - ELEMENTS AND PROTEINS IMAGING BY LA-ICP-MS: MEDICAL AND PHARMACEUTICAL RESEARCH
KW  - Bioimaging
KW  - laser ablation
KW  - protein detection
KW  - element distribution
UR  - https://www.vallhebron.com/actualitat/agenda/vall-dhebron-talks-vhir-elements-and-proteins-imaging-la-icp-ms-medical-and-pharmaceutical-research
N2  - In the last decades, increased interest in imaging of elements/metals/proteins distribution, mainly in pharmaceutical, biomedicine research, or life science with direct application of research to specific problem/disease [1-3]. The main emphasis of this contribution is to demonstrate the unique method of laser ablation with inductively coupled plasma and mass spectrometry (LA-ICP-MS). It is a well-established method for multi-elemental analysis of elements at trace and ultra-trace. Nowadays, it is also starting to be used as a technique for the simultaneous specific determination of the protein of interest, which opens up the possibility of achieving the so-called multiplex analysis. Protein determination, protein imaging and finally their distribution in clinical samples are presented. Proteins are imaged using an immunohistochemical method (binding nanoparticle-labeled antibodies to a specific protein) and LA-ICP-MS. The main advantages of utilizing LA-ICP-MS are the acquisition of comprehensive (Metallo)proteomic information about the tissue of a given disease (in this case, cancer or stroke) or low detection limits compared to other conventionally employed protein imaging techniques in combination with multiplex analysis of one sample.
ER  -

KUCHYNKA, Michaela, Marcela VLČNOVSKÁ, Kim KALLALA, Jan BISKUPIČ, Jana HLOŽKOVÁ, Peter SCHEER, Tereza PADRTOVÁ, Radka OPATŘILOVÁ, Markéta VACULOVIČOVÁ, Viktor KANICKÝ, Michal MASAŘÍK and Tomáš VACULOVIČ. ELEMENTS AND PROTEINS IMAGING BY LA-ICP-MS: MEDICAL AND PHARMACEUTICAL RESEARCH. In \textit{Vyzvaná přednáška na výzkumném institutu - Vall d'Hebron Talks by VHIR}. 2023.

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