In the last decades, increased interest in imaging of elements/metals/proteins distribution, mainly in pharmaceutical, biomedicine research, or life science with direct application of research to specific problem/disease [1-3]. The main emphasis of this contribution is to demonstrate the unique method of laser ablation with inductively coupled plasma and mass spectrometry (LA-ICP-MS). It is a well-established method for multi-elemental analysis of elements at trace and ultra-trace. Nowadays, it is also starting to be used as a technique for the simultaneous specific determination of the protein of interest, which opens up the possibility of achieving the so-called multiplex analysis. Protein determination, protein imaging and finally their distribution in clinical samples are presented. Proteins are imaged using an immunohistochemical method (binding nanoparticle-labeled antibodies to a specific protein) and LA-ICP-MS. The main advantages of utilizing LA-ICP-MS are the acquisition of comprehensive (Metallo)proteomic information about the tissue of a given disease (in this case, cancer or stroke) or low detection limits compared to other conventionally employed protein imaging techniques in combination with multiplex analysis of one sample.