NANO-SAVERS, POTENTIAL THERANOSTICS FOR STROKE DETECTION AND
TREATMENT: INVESTIGATION OF THEIR PHARMACOKINETICS

k 2023

NANO-SAVERS, POTENTIAL THERANOSTICS FOR STROKE DETECTION AND TREATMENT: INVESTIGATION OF THEIR PHARMACOKINETICS

KUCHYNKA, Michaela, Tereza PADRTOVÁ, Peter SCHEER, Jana HLOŽKOVÁ, Radka OPATŘILOVÁ et. al.

Basic information

Original name

NANO-SAVERS, POTENTIAL THERANOSTICS FOR STROKE DETECTION AND TREATMENT: INVESTIGATION OF THEIR PHARMACOKINETICS

Authors

KUCHYNKA, Michaela (203 Czech Republic, guarantor, belonging to the institution), Tereza PADRTOVÁ (203 Czech Republic, belonging to the institution), Peter SCHEER (203 Czech Republic, belonging to the institution), Jana HLOŽKOVÁ (203 Czech Republic, belonging to the institution), Radka OPATŘILOVÁ (203 Czech Republic, belonging to the institution), Marcela VLČNOVSKÁ (203 Czech Republic, belonging to the institution), Markéta VACULOVIČOVÁ (203 Czech Republic), Jan BISKUPIČ (203 Czech Republic, belonging to the institution), Viktor KANICKÝ (203 Czech Republic, belonging to the institution), Hossein MOHAMMADPOUR (826 United Kingdom of Great Britain and Northern Ireland), Tomáš ZIKMUND (203 Czech Republic), Jozef KAISER (203 Czech Republic) and Tomáš VACULOVIČ (203 Czech Republic, belonging to the institution)

Edition

European Winter Conference on Plasma Spectrochemistry, 2023

Other information

Language

English

Type of outcome

Prezentace na konferencích

Field of Study

10406 Analytical chemistry

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

European Winter Conference on Plasma Spectrochemistry

RIV identification code

RIV/00216224:14160/23:00134714

Organization unit

Faculty of Pharmacy

ISBN

978-961-6104-85-2

Keywords (in Czech)

biologické zobrazování; laserová ablace; detekce proteinu; prvková distribuce

Keywords in English

Bioimaging; laser ablation; protein detection; element distribution

Abstract

V originále

In the last decades, increased interest in imaging of elements/metals/proteins distribution, mainly in pharmaceutical, biomedicine research, or life science with direct application of research to specific problem/disease [1-3]. As WHO data indicates, cerebrovascular events are one of the main causes of mortality in developed nations. The key to success is time (as short as possible). Biodegradable iodinated nanoparticles (IoNPs) could be an invaluable addition to the existing contrast agents used in medicine. A complete investigation into the pharmacokinetics and dynamics of Ion-based Nanoparticles (IoNPs) is essential to address this issue. With the combination of CT and LA-ICP-MS imaging, this comprehensive evaluation verifies the treatment strategy's overall success, positioning LA-ICP-MS as a potential "gold standard" imaging method for researching and applying IoNPs in theranostics. Nowadays, LA-ICP-MS is also starting to be used as a technique for the simultaneous specific determination of the protein of interest, which opens up the possibility of achieving the so-called multiplex analysis. Protein determination, protein imaging and finally their distribution in clinical samples are presented. Proteins are imaged using an immunohistochemical method (binding nanoparticle-labeled antibodies to a specific protein) and LA-ICP-MS. The main advantages of utilizing LA-ICP-MS are the acquisition of comprehensive (Metallo)proteomic information about the tissue of a given disease (in this case, cancer or stroke) or low detection limits compared to other conventionally employed protein imaging techniques in combination with multiplex analysis of one sample.

Links

MUNI/R/1568/2020, interní kód MU

Name: Study the effectiveness of anticancer drugs (Acronym: STEAD)

Investor: Masaryk University, CAREER RESTART

NU21-08-00510, research and development project

Name: Biokompatibilní nanočástice jako cílené systémy pro dodávání léčiv a teranostik pro léčbu cévní mozkové příhody

Investor: Ministry of Health of the CR

Citovat

KUCHYNKA, Michaela, Tereza PADRTOVÁ, Peter SCHEER, Jana HLOŽKOVÁ, Radka OPATŘILOVÁ, Marcela VLČNOVSKÁ, Markéta VACULOVIČOVÁ, Jan BISKUPIČ, Viktor KANICKÝ, Hossein MOHAMMADPOUR, Tomáš ZIKMUND, Jozef KAISER and Tomáš VACULOVIČ. NANO-SAVERS, POTENTIAL THERANOSTICS FOR STROKE DETECTION AND TREATMENT: INVESTIGATION OF THEIR PHARMACOKINETICS. In European Winter Conference on Plasma Spectrochemistry. 2023. ISBN 978-961-6104-85-2.

@proceedings{2383663,
   author = {Kuchynka, Michaela and Padrtová, Tereza and Scheer, Peter and Hložková, Jana and Opatřilová, Radka and Vlčnovská, Marcela and Vaculovičová, Markéta and Biskupič, Jan and Kanický, Viktor and Mohammadpour, Hossein and Zikmund, Tomáš and Kaiser, Jozef and Vaculovič, Tomáš},
   booktitle = {European Winter Conference on Plasma Spectrochemistry},
   keywords = {Bioimaging; laser ablation; protein detection; element distribution},
   language = {eng},
   isbn = {978-961-6104-85-2},
   title = {NANO-SAVERS, POTENTIAL THERANOSTICS FOR STROKE DETECTION AND TREATMENT: INVESTIGATION OF THEIR PHARMACOKINETICS},
   url = {https://ewcps2023.si/index.html},
   year = {2023}
}

TY  - CONF
ID  - 2383663
AU  - Kuchynka, Michaela - Padrtová, Tereza - Scheer, Peter - Hložková, Jana - Opatřilová, Radka - Vlčnovská, Marcela - Vaculovičová, Markéta - Biskupič, Jan - Kanický, Viktor - Mohammadpour, Hossein - Zikmund, Tomáš - Kaiser, Jozef - Vaculovič, Tomáš
PY  - 2023
TI  - NANO-SAVERS, POTENTIAL THERANOSTICS FOR STROKE DETECTION AND TREATMENT: INVESTIGATION OF THEIR PHARMACOKINETICS
SN  - 9789616104852
KW  - Bioimaging
KW  - laser ablation
KW  - protein detection
KW  - element distribution
UR  - https://ewcps2023.si/index.html
N2  - In the last decades, increased interest in imaging of elements/metals/proteins distribution, mainly in pharmaceutical, biomedicine research, or life science with direct application of research to specific problem/disease [1-3]. As WHO data indicates, cerebrovascular events are one of the main causes of mortality in developed nations. The key to success is time (as short as possible). Biodegradable iodinated nanoparticles (IoNPs) could be an invaluable addition to the existing contrast agents used in medicine. A complete investigation into the pharmacokinetics and dynamics of Ion-based Nanoparticles (IoNPs) is essential to address this issue. With the combination of CT and LA-ICP-MS imaging, this comprehensive evaluation verifies the treatment strategy's overall success, positioning LA-ICP-MS as a potential "gold standard" imaging method for researching and applying IoNPs in theranostics. Nowadays, LA-ICP-MS is also starting to be used as a technique for the simultaneous specific determination of the protein of interest, which opens up the possibility of achieving the so-called multiplex analysis. Protein determination, protein imaging and finally their distribution in clinical samples are presented. Proteins are imaged using an immunohistochemical method (binding nanoparticle-labeled antibodies to a specific protein) and LA-ICP-MS. The main advantages of utilizing LA-ICP-MS are the acquisition of comprehensive (Metallo)proteomic information about the tissue of a given disease (in this case, cancer or stroke) or low detection limits compared to other conventionally employed protein imaging techniques in combination with multiplex analysis of one sample.
ER  -

KUCHYNKA, Michaela, Tereza PADRTOVÁ, Peter SCHEER, Jana HLOŽKOVÁ, Radka OPATŘILOVÁ, Marcela VLČNOVSKÁ, Markéta VACULOVIČOVÁ, Jan BISKUPIČ, Viktor KANICKÝ, Hossein MOHAMMADPOUR, Tomáš ZIKMUND, Jozef KAISER and Tomáš VACULOVIČ. NANO-SAVERS, POTENTIAL THERANOSTICS FOR STROKE DETECTION AND TREATMENT: INVESTIGATION OF THEIR PHARMACOKINETICS. In \textit{European Winter Conference on Plasma Spectrochemistry}. 2023. ISBN~978-961-6104-85-2.

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