Functional differentiation of Sec13 paralogues in the
euglenozoan protists

J 2023

Functional differentiation of Sec13 paralogues in the euglenozoan protists

FAKTOROVA, Drahomira, Kristina ZAHONOVA, Corinna BENZ, Joel B DACKS, Mark C FIELD et. al.

Basic information

Original name

Functional differentiation of Sec13 paralogues in the euglenozoan protists

Authors

FAKTOROVA, Drahomira (guarantor), Kristina ZAHONOVA, Corinna BENZ, Joel B DACKS, Mark C FIELD and Julius LUKES

Edition

Open Biology, LONDON, ROYAL SOC, 2023, 2046-2441

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.800 in 2022

RIV identification code

RIV/00216224:90242/23:00133778

DOI

http://dx.doi.org/10.1098/rsob.220364

UT WoS

001005301600003

Keywords in English

Diplonema; coatomer; membrane trafficking; nuclear pore complex; SEA; GATOR complex; paralogue expansion

Abstract

V originále

The beta-propeller protein Sec13 plays roles in at least three distinct processes by virtue of being a component of the COPII endoplasmic reticulum export vesicle coat, the nuclear pore complex (NPC) and the Seh1-associated (SEA)/GATOR nutrient-sensing complex. This suggests that regulatory mechanisms coordinating these cellular activities may operate via Sec13. The NPC, COPII and SEA/GATOR are all ancient features of eukaryotic cells, and in the vast majority of eukaryotes, a single Sec13 gene is present. Here we report that the Euglenozoa, a lineage encompassing the diplonemid, kinetoplastid and euglenid protists, possess two Sec13 paralogues. Furthermore, based on protein interactions and localization studies we show that in diplonemids Sec13 functions are divided between the Sec13a and Sec13b paralogues. Specifically, Sec13a interacts with COPII and the NPC, while Sec13b interacts with Sec16 and components of the SEA/GATOR complex. We infer that euglenozoan Sec13a is responsible for NPC functions and canonical anterograde transport activities while Sec13b acts within nutrient and autophagy-related pathways, indicating a fundamentally distinct organization of coatomer complexes in euglenozoan flagellates.

Links

90242, large research infrastructures

Name: CIISB III

Citovat

FAKTOROVA, Drahomira, Kristina ZAHONOVA, Corinna BENZ, Joel B DACKS, Mark C FIELD and Julius LUKES. Functional differentiation of Sec13 paralogues in the euglenozoan protists. Open Biology. LONDON: ROYAL SOC, 2023, vol. 13, No 6, p. 1-15. ISSN 2046-2441. Available from: https://dx.doi.org/10.1098/rsob.220364.

@article{2384237,
   author = {Faktorova, Drahomira and Zahonova, Kristina and Benz, Corinna and Dacks, Joel B and Field, Mark C and Lukes, Julius},
   article_location = {LONDON},
   article_number = {6},
   doi = {http://dx.doi.org/10.1098/rsob.220364},
   keywords = {Diplonema; coatomer; membrane trafficking; nuclear pore complex; SEA; GATOR complex; paralogue expansion},
   language = {eng},
   issn = {2046-2441},
   journal = {Open Biology},
   title = {Functional differentiation of Sec13 paralogues in the euglenozoan protists},
   url = {https://royalsocietypublishing.org/doi/10.1098/rsob.220364},
   volume = {13},
   year = {2023}
}

TY  - JOUR
ID  - 2384237
AU  - Faktorova, Drahomira - Zahonova, Kristina - Benz, Corinna - Dacks, Joel B - Field, Mark C - Lukes, Julius
PY  - 2023
TI  - Functional differentiation of Sec13 paralogues in the euglenozoan protists
JF  - Open Biology
VL  - 13
IS  - 6
SP  - 1-15
EP  - 1-15
PB  - ROYAL SOC
SN  - 20462441
KW  - Diplonema
KW  - coatomer
KW  - membrane trafficking
KW  - nuclear pore complex
KW  - SEA
KW  - GATOR complex
KW  - paralogue expansion
UR  - https://royalsocietypublishing.org/doi/10.1098/rsob.220364
N2  - The beta-propeller protein Sec13 plays roles in at least three distinct processes by virtue of being a component of the COPII endoplasmic reticulum export vesicle coat, the nuclear pore complex (NPC) and the Seh1-associated (SEA)/GATOR nutrient-sensing complex. This suggests that regulatory mechanisms coordinating these cellular activities may operate via Sec13. The NPC, COPII and SEA/GATOR are all ancient features of eukaryotic cells, and in the vast majority of eukaryotes, a single Sec13 gene is present. Here we report that the Euglenozoa, a lineage encompassing the diplonemid, kinetoplastid and euglenid protists, possess two Sec13 paralogues. Furthermore, based on protein interactions and localization studies we show that in diplonemids Sec13 functions are divided between the Sec13a and Sec13b paralogues. Specifically, Sec13a interacts with COPII and the NPC, while Sec13b interacts with Sec16 and components of the SEA/GATOR complex. We infer that euglenozoan Sec13a is responsible for NPC functions and canonical anterograde transport activities while Sec13b acts within nutrient and autophagy-related pathways, indicating a fundamentally distinct organization of coatomer complexes in euglenozoan flagellates.
ER  -

FAKTOROVA, Drahomira, Kristina ZAHONOVA, Corinna BENZ, Joel B DACKS, Mark C FIELD and Julius LUKES. Functional differentiation of Sec13 paralogues in the euglenozoan protists. \textit{Open Biology}. LONDON: ROYAL SOC, 2023, vol.~13, No~6, p.~1-15. ISSN~2046-2441. Available from: https://dx.doi.org/10.1098/rsob.220364.

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