Supported polymer/lipid hybrid bilayers formation resembles a
lipid-like dynamic by reducing the molecular weight of the
polymer

J 2021

Supported polymer/lipid hybrid bilayers formation resembles a lipid-like dynamic by reducing the molecular weight of the polymer

BELLO, Gianluca, Francesca CAVALLINI, Lea Ann DAILEY and Eva-Kathrin EHMOSER

Basic information

Original name

Supported polymer/lipid hybrid bilayers formation resembles a lipid-like dynamic by reducing the molecular weight of the polymer

Authors

BELLO, Gianluca (guarantor), Francesca CAVALLINI, Lea Ann DAILEY and Eva-Kathrin EHMOSER

Edition

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, AMSTERDAM, ELSEVIER SCIENCE BV, 2021, 0005-2736

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.019

RIV identification code

RIV/00216224:90127/21:00133782

DOI

http://dx.doi.org/10.1016/j.bbamem.2020.183472

UT WoS

000594121100015

Keywords in English

Supported bilayer; QCM-D; SANS; Hybrid lipid-polymer bilayer; Langmuir films; Amphiphilic copolymer

Abstract

V originále

Amphiphilic block copolymers form self-assembled bilayers even in combination with phospholipids. They represent an attractive alternative to native lipid-based membrane systems for supported bilayer formation with applications in biomedical research, sensoring and drug delivery. Their enhanced stability and excellent mechanical properties are linked to their higher molecular weight which generates thicker bilayers. Hypothesis: It is hypothesized that reducing the molecular weight of the polymer facilitates the formation of a thinner, more homogeneous polymer/lipid hybrid bilayer which would benefit the formation of supported bilayers on silicon oxide. Experiment: We investigated hybrid bilayers composed of mixtures of 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine and increasing amounts of a low molecular weight polybutadiene-b-polyethylene oxide copolymer (1050 g/mol). By assessing the bilayer thickness and the molecular packing behavior we sought to demonstrate how reducing the polymer molecular weight increases the tendency to form supported hybrid bilayers in a lipid-like manner. Findings: The formation of a supported hybrid bilayers occurs at polymer contents < 70 mol% in a lipid-like fashion and is proportional to the cohesive forces between the bilayer components and inversely related to the bilayer hydrophobic core thickness and the extended brush regime of the PEGylated polymeric headgroup.

Links

90127, large research infrastructures

Name: CIISB II

Citovat

BELLO, Gianluca, Francesca CAVALLINI, Lea Ann DAILEY and Eva-Kathrin EHMOSER. Supported polymer/lipid hybrid bilayers formation resembles a lipid-like dynamic by reducing the molecular weight of the polymer. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES. AMSTERDAM: ELSEVIER SCIENCE BV, 2021, vol. 1863, No 1, p. 1-8. ISSN 0005-2736. Available from: https://dx.doi.org/10.1016/j.bbamem.2020.183472.

@article{2384339,
   author = {Bello, Gianluca and Cavallini, Francesca and Dailey, Lea Ann and Ehmoser, EvaandKathrin},
   article_location = {AMSTERDAM},
   article_number = {1},
   doi = {http://dx.doi.org/10.1016/j.bbamem.2020.183472},
   keywords = {Supported bilayer; QCM-D; SANS; Hybrid lipid-polymer bilayer; Langmuir films; Amphiphilic copolymer},
   language = {eng},
   issn = {0005-2736},
   journal = {BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES},
   title = {Supported polymer/lipid hybrid bilayers formation resembles a lipid-like dynamic by reducing the molecular weight of the polymer},
   url = {https://www.sciencedirect.com/science/article/pii/S0005273620303151?via%3Dihub},
   volume = {1863},
   year = {2021}
}

TY  - JOUR
ID  - 2384339
AU  - Bello, Gianluca - Cavallini, Francesca - Dailey, Lea Ann - Ehmoser, Eva-Kathrin
PY  - 2021
TI  - Supported polymer/lipid hybrid bilayers formation resembles a lipid-like dynamic by reducing the molecular weight of the polymer
JF  - BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
VL  - 1863
IS  - 1
SP  - 1-8
EP  - 1-8
PB  - ELSEVIER SCIENCE BV
SN  - 00052736
KW  - Supported bilayer
KW  - QCM-D
KW  - SANS
KW  - Hybrid lipid-polymer bilayer
KW  - Langmuir films
KW  - Amphiphilic copolymer
UR  - https://www.sciencedirect.com/science/article/pii/S0005273620303151?via%3Dihub
N2  - Amphiphilic block copolymers form self-assembled bilayers even in combination with phospholipids. They represent an attractive alternative to native lipid-based membrane systems for supported bilayer formation with applications in biomedical research, sensoring and drug delivery. Their enhanced stability and excellent mechanical properties are linked to their higher molecular weight which generates thicker bilayers. Hypothesis: It is hypothesized that reducing the molecular weight of the polymer facilitates the formation of a thinner, more homogeneous polymer/lipid hybrid bilayer which would benefit the formation of supported bilayers on silicon oxide. Experiment: We investigated hybrid bilayers composed of mixtures of 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine and increasing amounts of a low molecular weight polybutadiene-b-polyethylene oxide copolymer (1050 g/mol). By assessing the bilayer thickness and the molecular packing behavior we sought to demonstrate how reducing the polymer molecular weight increases the tendency to form supported hybrid bilayers in a lipid-like manner. Findings: The formation of a supported hybrid bilayers occurs at polymer contents < 70 mol% in a lipid-like fashion and is proportional to the cohesive forces between the bilayer components and inversely related to the bilayer hydrophobic core thickness and the extended brush regime of the PEGylated polymeric headgroup.
ER  -

BELLO, Gianluca, Francesca CAVALLINI, Lea Ann DAILEY and Eva-Kathrin EHMOSER. Supported polymer/lipid hybrid bilayers formation resembles a lipid-like dynamic by reducing the molecular weight of the polymer. \textit{BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES}. AMSTERDAM: ELSEVIER SCIENCE BV, 2021, vol.~1863, No~1, p.~1-8. ISSN~0005-2736. Available from: https://dx.doi.org/10.1016/j.bbamem.2020.183472.

Detailed Information on Publication Record