The cryo-EM structure of the S-layer deinoxanthin-binding
complex of Deinococcus radiodurans informs
properties of its environmental interactions

J 2022

The cryo-EM structure of the S-layer deinoxanthin-binding complex of Deinococcus radiodurans informs properties of its environmental interactions

FARCI, Domenica, Patrycja HANIEWICZ, de Sanctis DANIELE, Luca IESU, Sami KEREICHE et. al.

Basic information

Original name

The cryo-EM structure of the S-layer deinoxanthin-binding complex of Deinococcus radiodurans informs properties of its environmental interactions

Authors

FARCI, Domenica (guarantor), Patrycja HANIEWICZ, de Sanctis DANIELE, Luca IESU, Sami KEREICHE, Mathias WINTERHALTER and Dario PIANO

Edition

International Journal of Biological Chemistry, AMSTERDAM, ANSInet, 2022, 1083-351X

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.800

RIV identification code

RIV/00216224:90127/22:00133791

DOI

http://dx.doi.org/10.1016/j.jbc.2022.102031

UT WoS

000829588600004

Keywords in English

PROTEINS; MICROSCOPE; RESOLUTION; Deinococcus radiodurans; S-layer; deinoxanthin-binding complex;

Abstract

V originále

The radiation-resistant bacterium Deinococcus radiodurans is known as the world's toughest bacterium. The S-layer of D. radiodurans, consisting of several proteins on the surface of the cellular envelope and intimately associated with the outer membrane, has therefore been useful as a model for structural and functional studies. Its main proteinaceous unit, the S-layer deinoxanthin-binding complex (SDBC), is a hetero-oligomeric assembly known to contribute to the resistance against environmental stress and have porin functional features; however, its precise structure is unknown. Here, we resolved the structure of the SDBC at -2.5 angstrom resolution by cryo-EM and assigned the sequence of its main subunit, the protein DR_2577. This structure is characterized by a pore region, a massive beta-barrel organization, a stalk region consisting of a trimeric coiled coil, and a collar region at the base of the stalk. We show that each monomer binds three Cu ions and one Fe ion and retains one deinoxanthin molecule and two phosphoglycolipids, all exclusive to D. radiodurans. Finally, electrophysiological characterization of the SDBC shows that it exhibits transport properties with several amino acids. Taken together, these results highlight the SDBC as a robust structure displaying both protection and sieving functions that facilitates exchanges with the environment.

Links

90127, large research infrastructures

Name: CIISB II

Citovat

FARCI, Domenica, Patrycja HANIEWICZ, de Sanctis DANIELE, Luca IESU, Sami KEREICHE, Mathias WINTERHALTER and Dario PIANO. The cryo-EM structure of the S-layer deinoxanthin-binding complex of Deinococcus radiodurans informs properties of its environmental interactions. International Journal of Biological Chemistry. AMSTERDAM: ANSInet, 2022, vol. 298, No 6, p. 1-8. ISSN 1083-351X. Available from: https://dx.doi.org/10.1016/j.jbc.2022.102031.

@article{2384837,
 author = {Farci, Domenica and Haniewicz, Patrycja and Daniele, de Sanctis and Iesu, Luca and Kereiche, Sami and Winterhalter, Mathias and Piano, Dario},
 article_location = {AMSTERDAM},
 article_number = {6},
 doi = {http://dx.doi.org/10.1016/j.jbc.2022.102031},
 keywords = {PROTEINS; MICROSCOPE; RESOLUTION; Deinococcus radiodurans; S-layer; deinoxanthin-binding complex;},
 language = {eng},
 issn = {1083-351X},
 journal = {International Journal of Biological Chemistry},
 title = {The cryo-EM structure of the S-layer deinoxanthin-binding complex of Deinococcus radiodurans informs properties of its environmental interactions},
 url = {https://www.sciencedirect.com/science/article/pii/S0021925822004719?via%3Dihub},
 volume = {298},
 year = {2022}
}

TY - JOUR
ID - 2384837
AU - Farci, Domenica - Haniewicz, Patrycja - Daniele, de Sanctis - Iesu, Luca - Kereiche, Sami - Winterhalter, Mathias - Piano, Dario
PY - 2022
TI - The cryo-EM structure of the S-layer deinoxanthin-binding complex of Deinococcus radiodurans informs properties of its environmental interactions
JF - International Journal of Biological Chemistry
VL - 298
IS - 6
SP - 1-8
EP - 1-8
PB - ANSInet
SN - 1083351X
KW - PROTEINS
KW - MICROSCOPE
KW - RESOLUTION
KW - Deinococcus radiodurans
KW - S-layer
KW - deinoxanthin-binding complex;
UR - https://www.sciencedirect.com/science/article/pii/S0021925822004719?via%3Dihub
N2 - The radiation-resistant bacterium Deinococcus radiodurans is known as the world's toughest bacterium. The S-layer of D. radiodurans, consisting of several proteins on the surface of the cellular envelope and intimately associated with the outer membrane, has therefore been useful as a model for structural and functional studies. Its main proteinaceous unit, the S-layer deinoxanthin-binding complex (SDBC), is a hetero-oligomeric assembly known to contribute to the resistance against environmental stress and have porin functional features; however, its precise structure is unknown. Here, we resolved the structure of the SDBC at -2.5 angstrom resolution by cryo-EM and assigned the sequence of its main subunit, the protein DR_2577. This structure is characterized by a pore region, a massive beta-barrel organization, a stalk region consisting of a trimeric coiled coil, and a collar region at the base of the stalk. We show that each monomer binds three Cu ions and one Fe ion and retains one deinoxanthin molecule and two phosphoglycolipids, all exclusive to D. radiodurans. Finally, electrophysiological characterization of the SDBC shows that it exhibits transport properties with several amino acids. Taken together, these results highlight the SDBC as a robust structure displaying both protection and sieving functions that facilitates exchanges with the environment.
ER -

FARCI, Domenica, Patrycja HANIEWICZ, de Sanctis DANIELE, Luca IESU, Sami KEREICHE, Mathias WINTERHALTER and Dario PIANO. The cryo-EM structure of the S-layer deinoxanthin-binding complex of\&{}lt;i\&{}gt; Deinococcus\&{}lt;/i\&{}gt;\&{}lt;i\&{}gt; radiodurans\&{}lt;/i\&{}gt; informs properties of its environmental interactions. \textit{International Journal of Biological Chemistry}. AMSTERDAM: ANSInet, 2022, vol.~298, No~6, p.~1-8. ISSN~1083-351X. Available from: https://dx.doi.org/10.1016/j.jbc.2022.102031.

Detailed Information on Publication Record

The cryo-EM structure of the S-layer deinoxanthin-binding complex of<i> Deinococcus</i><i> radiodurans</i> informs properties of its environmental interactions