Detailed Information on Publication Record
2023
Super resolution microscopy based on spinning-disc and optical photon reassignment
EŠNER, Milan and Petra KUČEROVÁBasic information
Original name
Super resolution microscopy based on spinning-disc and optical photon reassignment
Authors
EŠNER, Milan (203 Czech Republic, guarantor, belonging to the institution) and Petra KUČEROVÁ (203 Czech Republic, belonging to the institution)
Edition
2023
Other information
Language
English
Type of outcome
Uspořádání workshopu
Field of Study
10400 1.4 Chemical sciences
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
RIV identification code
RIV/00216224:14740/23:00133814
Organization unit
Central European Institute of Technology
Keywords in English
CEITEC Core Facility CELLIM; spinning-disk confocal microscopy; Nipkow disk; Olympus Super Resolution
Tags
International impact, Reviewed
Změněno: 20/3/2024 15:20, Mgr. Eva Dubská
Abstract
V originále
Spinning-disk confocal microscopy is a proven technology for investigating 3D structures of biological specimens. With the help of a newly modified Nipkow disk (SoRa), which comprises pinholes and micro-lenses on the front and back, respectively, emitted photons from the specimen can be optically reassigned to the most probable locations they originate from. Data reliability is ensured owing to our Olympus Super Resolution (OSR) technology. Based on optical theory, our OSR processing algorithms are capable of obtaining a spatial resolution of 120 nm, providing highly reliable results below the diffraction limit. Deep tissue observation is achieved using silicone immersion objectives and through the optical sectioning capability of confocal microscopes.
Links
| LM2023050, research and development project |
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