EŠNER, Milan and Petra KUČEROVÁ. Super resolution microscopy based on spinning-disc and optical photon reassignment. 2023.
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Basic information
Original name Super resolution microscopy based on spinning-disc and optical photon reassignment
Authors EŠNER, Milan (203 Czech Republic, guarantor, belonging to the institution) and Petra KUČEROVÁ (203 Czech Republic, belonging to the institution).
Edition 2023.
Other information
Original language English
Type of outcome Organization of a workshop
Field of Study 10400 1.4 Chemical sciences
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
WWW URL
RIV identification code RIV/00216224:14740/23:00133814
Organization unit Central European Institute of Technology
Keywords in English CEITEC Core Facility CELLIM; spinning-disk confocal microscopy; Nipkow disk; Olympus Super Resolution
Tags CF CELLIM, rivok
Tags International impact, Reviewed
Changed by Changed by: Mgr. Eva Dubská, učo 77638. Changed: 20/3/2024 15:20.
Abstract
Spinning-disk confocal microscopy is a proven technology for investigating 3D structures of biological specimens. With the help of a newly modified Nipkow disk (SoRa), which comprises pinholes and micro-lenses on the front and back, respectively, emitted photons from the specimen can be optically reassigned to the most probable locations they originate from. Data reliability is ensured owing to our Olympus Super Resolution (OSR) technology. Based on optical theory, our OSR processing algorithms are capable of obtaining a spatial resolution of 120 nm, providing highly reliable results below the diffraction limit. Deep tissue observation is achieved using silicone immersion objectives and through the optical sectioning capability of confocal microscopes.
Links
LM2023050, research and development projectName: Národní infrastruktura pro biologické a medicínské zobrazování
Investor: Ministry of Education, Youth and Sports of the CR, Czech BioImaging: National research infrastructure for biological and medical imaging
PrintDisplayed: 7/10/2024 22:08