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@misc{2385325, author = {Ešner, Milan and Kučerová, Petra}, keywords = {CEITEC Core Facility CELLIM; spinning-disk confocal microscopy; Nipkow disk; Olympus Super Resolution}, language = {eng}, title = {Super resolution microscopy based on spinning-disc and optical photon reassignment}, url = {https://www.ceitec.cz/super-resolution-microscopy-based-on-spinning-disc-and-optical-photon-reassignment/a4660}, year = {2023} }
TY - CONF ID - 2385325 AU - Ešner, Milan - Kučerová, Petra PY - 2023 TI - Super resolution microscopy based on spinning-disc and optical photon reassignment KW - CEITEC Core Facility CELLIM KW - spinning-disk confocal microscopy KW - Nipkow disk KW - Olympus Super Resolution UR - https://www.ceitec.cz/super-resolution-microscopy-based-on-spinning-disc-and-optical-photon-reassignment/a4660 N2 - Spinning-disk confocal microscopy is a proven technology for investigating 3D structures of biological specimens. With the help of a newly modified Nipkow disk (SoRa), which comprises pinholes and micro-lenses on the front and back, respectively, emitted photons from the specimen can be optically reassigned to the most probable locations they originate from. Data reliability is ensured owing to our Olympus Super Resolution (OSR) technology. Based on optical theory, our OSR processing algorithms are capable of obtaining a spatial resolution of 120 nm, providing highly reliable results below the diffraction limit. Deep tissue observation is achieved using silicone immersion objectives and through the optical sectioning capability of confocal microscopes. ER -
EŠNER, Milan and Petra KUČEROVÁ. \textit{Super resolution microscopy based on spinning-disc and optical photon reassignment}. 2023.
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