Detailed Information on Publication Record
2024
Comparison of microbial communities and the profile of sulfate-reducing bacteria in patients with ulcerative colitis and their association with bowel diseases: a pilot study
KUSHKEVYCH, Ivan, Kristýna MARTÍNKOVÁ, Lenka MRÁKOVÁ, Francesco GIUDICI, Simone BALDI et. al.Basic information
Original name
Comparison of microbial communities and the profile of sulfate-reducing bacteria in patients with ulcerative colitis and their association with bowel diseases: a pilot study
Authors
KUSHKEVYCH, Ivan (203 Czech Republic, guarantor, belonging to the institution), Kristýna MARTÍNKOVÁ (203 Czech Republic, belonging to the institution), Lenka MRÁKOVÁ (203 Czech Republic, belonging to the institution), Francesco GIUDICI, Simone BALDI, David NOVÁK (203 Czech Republic, belonging to the institution), Márió GAJDÁCS, Monika VÍTĚZOVÁ (203 Czech Republic, belonging to the institution), Dani DORDEVIC, Amedeo AMEDEI and Simon K.-M. R. RITTMANN
Edition
Microbial Cell, Shared Science Publishers OG, 2024, 2311-2638
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10606 Microbiology
Country of publisher
Austria
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 4.600 in 2022
Organization unit
Faculty of Science
UT WoS
001187090800001
Keywords in English
gut microbiota; ulcerative colitis; gut dysbiosis; sulfate-reducing bacteria; inflammatory bowel disease; 16S rRNA gene sequencing
Tags
Tags
International impact, Reviewed
Změněno: 5/4/2024 09:46, Mgr. Marie Šípková, DiS.
Abstract
V originále
Considerable evidence has accumulated regarding the molecular relationship between gut microbiota (GM) composition and the onset (clinical presentation and prognosis of ulcerative colitis (UC)). In addition, it is well documented that short-chain fatty acid (SCFA)-producing bacteria may play a fundamental role in maintaining an anti-inflammatory intestinal homeostasis, but sulfate- and sulfite reducing bacteria may be responsible for the production of toxic metabolites, such as hydrogen sulfide and acetate. Hence, the present study aimed to assess the GM composition – focusing on sulfate-reducing bacteria (SRB) – in patients with severe, severe-active and moderate UC. Each one of the six enrolled patients provided two stool samples in the following way: one sample was cultivated in a modified SRB-medium before 16S rRNA sequencing and the other was not cultivated. Comparative phylogenetic analysis was conducted on each sample. Percentage of detected gut microbial genera showed considerable variation based on the patients’ disease severity and cultivation in the SRB medium. In detail, samples without cultivation from patients with moderate UC showed a high abundance of the genera Bacteroides, Bifidobacterium and Ruminococcus, but after SRB cultivation, the dominant genera were Bacteroides, Klebsiella and Bilophila. On the other hand, before SRB cultivation, the main represented genera in patients with severe UC were Escherichia-Shigella, Proteus, Methanothermobacter and Methanobacterium. However, after incubation in the SRB medium Bacteroides, Proteus, Alistipes and Lachnoclostridium were predominant. Information regarding GM compositional changes in UC patients may aid the development of novel therapeutic strategies (e.g., probiotic preparations containing specific bacterial strains) to counteract the mechanisms of virulence of harmful bacteria and the subsequent inflammatory response that is closely related to the pathogenesis of inflammatory bowel diseases.
Links
| MUNI/A/1280/2022, interní kód MU |
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