J 2024

Long-term dietary exposure to the non-steroidal anti-inflammatory drugs diclofenac and ibuprofen can affect the physiology of common carp (<i>Cyprinus carpio</i>) on multiple levels, even at "environmentally relevant" concentrations

MIKULA, Premysl, Aneta HOLLEROVA, Nikola HODKOVICOVA, Veronika DOUBKOVA, Petr MARSALEK et. al.

Basic information

Original name

Long-term dietary exposure to the non-steroidal anti-inflammatory drugs diclofenac and ibuprofen can affect the physiology of common carp (<i>Cyprinus carpio</i>) on multiple levels, even at "environmentally relevant" concentrations

Authors

MIKULA, Premysl, Aneta HOLLEROVA (203 Czech Republic), Nikola HODKOVICOVA (203 Czech Republic), Veronika DOUBKOVA (203 Czech Republic), Petr MARSALEK (203 Czech Republic), Aleš FRANC (203 Czech Republic, belonging to the institution), Lucie SEDLÁČKOVÁ (203 Czech Republic, belonging to the institution), Renata HESOVA (203 Czech Republic), Helena MODRA (203 Czech Republic), Zdenka SVOBODOVA (203 Czech Republic) and Jana BLAHOVA (203 Czech Republic)

Edition

Science of the Total Environment, AMSTERDAM, Elsevier, 2024, 0048-9697

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30104 Pharmacology and pharmacy

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 9.800 in 2022

Organization unit

Faculty of Pharmacy

DOI

UT WoS

001178917700001

Keywords in English

Fish; Haematological indices; Oxidative stress; Plasma biochemistry; Gene expression; Thyroid disruption

Tags

Tags

International impact, Reviewed
Změněno: 20/3/2024 12:33, Mgr. Daniela Černá

Abstract

V originále

The aim of the study was to evaluate the effects of emerging environmental contaminants, the non-steroidal anti-inflammatory drugs (NSAIDs) diclofenac (DCF) and ibuprofen (IBP), on physiological functions in juvenile common carp (Cyprinus carpio). Fish were exposed for 6 weeks, and for the first time, NSAIDs were administered through diet. Either substance was tested at two concentrations, 20 or 2000 mu g/kg, resulting in four different treatments (DCF 20, DCF 2000, IBP 20, IBP 2000). The effects on haematological and biochemical profiles, the biomarkers of oxidative stress, and endocrine disruption were studied, and changes in RNA transcription were also monitored to obtain a comprehensive picture of toxicity. Fish exposure to high concentrations of NSAIDs (DCF 2000, IBP 2000) elicited numerous statistically significant changes (p < 0.05) in the endpoints investigated, with DCF being almost always more efficient than IBP. Compared to control fish, a decrease in total leukocyte count attributed to relative lymphopenia was observed. Plasma concentrations of total proteins, ammonia, and thyroxine, and enzyme activities of alanine aminotransferase (ALT), aspartate aminotransferase, and alkaline phosphatase (ALP) were significantly elevated in either group, as were the activities of certain hepatic antioxidant enzymes (superoxide dismutase, glutathione-S-transferase) in the DCF 2000 group. The transcriptomic profile of selected genes in the tissues of exposed fish was affected as well. Significant changes in plasma total proteins, ammonia, ALT, and ALP, as well as in the transcription of genes related to thyroid function and the antioxidant defense of the organism, were found even in fish exposed to the lower DCF concentration (DCF 20). As it was chosen to match DCF concentrations commonly detected in aquatic invertebrates (i.e., the potential feed source of fish), it can be considered "environmentally relevant". Future research is necessary to shed more light on the dietary NSAID toxicity to fish.