HUBINSKÝ, Marcel, Javier Martín-Gómez JOSÉ, Emilio CERVANTES, Roman HOBZA and José Luís RODRÍGUEZ LORENZO. Seed shape and size of Silene latifolia, differences between sexes, and influence of the parental genome in hybrids with Silene dioica. Frontiers in Plant Science. Lausanne: Frontiers Media SA, 2024, vol. 15, March 2024, p. 1-13. ISSN 1664-462X. Available from: https://dx.doi.org/10.3389/fpls.2024.1297676.
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Basic information
Original name Seed shape and size of Silene latifolia, differences between sexes, and influence of the parental genome in hybrids with Silene dioica
Authors HUBINSKÝ, Marcel (703 Slovakia, guarantor, belonging to the institution), Javier Martín-Gómez JOSÉ (724 Spain), Emilio CERVANTES (724 Spain), Roman HOBZA (203 Czech Republic) and José Luís RODRÍGUEZ LORENZO (724 Spain).
Edition Frontiers in Plant Science, Lausanne, Frontiers Media SA, 2024, 1664-462X.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10611 Plant sciences, botany
Country of publisher Switzerland
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 5.600 in 2022
Organization unit Faculty of Science
Doi http://dx.doi.org/10.3389/fpls.2024.1297676
UT WoS 001189886900001
Keywords in English elliptical Fourier analysis; Morphometrics geometrics; plant hybrid; polyploidy; seed shape; Silene dioica; Silene latifolia; symmetry
Tags rivok
Tags International impact, Reviewed
Changed by Changed by: Mgr. Marie Šípková, DiS., učo 437722. Changed: 26/4/2024 13:11.
Abstract
Introduction: Plants undergo various natural changes that dramatically modify their genomes. One is polyploidization and the second is hybridization. Both are regarded as key factors in plant evolution and result in phenotypic differences in different plant organs. In Silene, we can find both examples in nature, and this genus has a seed shape diversity that has long been recognized as a valuable source of information for infrageneric classification. Methods: Morphometric analysis is a statistical study of shape and size and their covariations with other variables. Traditionally, seed shape description was limited to an approximate comparison with geometric figures (rounded, globular, reniform, or heart-shaped). Seed shape quantification has been based on direct measurements, such as area, perimeter, length, and width, narrowing statistical analysis. We used seed images and processed them to obtain silhouettes. We performed geometric morphometric analyses, such as similarity to geometric models and elliptic Fourier analysis, to study the hybrid offspring of S. latifolia and S. dioica. Results: We generated synthetic tetraploids of Silene latifolia and performed controlled crosses between diploid S. latifolia and Silene dioica to analyze seed morphology. After imaging capture and post-processing, statistical analysis revealed differences in seed size, but not in shape, between S. latifolia diploids and tetraploids, as well as some differences in shape among the parentals and hybrids. A detailed inspection using fluorescence microscopy allowed for the identification of shape differences in the cells of the seed coat. In the case of hybrids, differences were found in circularity and solidity. Overal seed shape is maternally regulated for both species, whereas cell shape cannot be associated with any of the sexes. Discussion: Our results provide additional tools useful for the combination of morphology with genetics, ecology or taxonomy. Seed shape is a robust indicator that can be used as a complementary tool for the genetic and phylogenetic analyses of Silene hybrid populations.
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