HILIC Separation Methods on Poly-Hydroxyl Stationary Phases for
Determination of Common Saccharides with Evaporative
Light-Scattering Detector and Rapid Determination of
Isomaltulose in Protein-Rich Food Supplements

J 2024

HILIC Separation Methods on Poly-Hydroxyl Stationary Phases for Determination of Common Saccharides with Evaporative Light-Scattering Detector and Rapid Determination of Isomaltulose in Protein-Rich Food Supplements

CRHA, Tomáš, Grace F ODEDINA and Jiří PAZOUREK

Basic information

Original name

HILIC Separation Methods on Poly-Hydroxyl Stationary Phases for Determination of Common Saccharides with Evaporative Light-Scattering Detector and Rapid Determination of Isomaltulose in Protein-Rich Food Supplements

Authors

CRHA, Tomáš (203 Czech Republic, belonging to the institution), Grace F ODEDINA (276 Germany) and Jiří PAZOUREK (203 Czech Republic, belonging to the institution)

Edition

Fluid - Particle Separations Journal, BASEL, American Filtration and Separations Society, 2024, 1541-9290

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30104 Pharmacology and pharmacy

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Organization unit

Faculty of Pharmacy

DOI

http://dx.doi.org/10.3390/separations11020045

UT WoS

001172378500001

Keywords in English

HILIC; ELSD; saccharides; sugar alcohols; isomaltulose (Palatinose); food supplements

Abstract

V originále

This article highlights the fundamental aspects of hydrophilic interaction liquid chromatography (HILIC) on poly-hydroxyl stationary phases to analyze non-derivatized mono- and disaccharides, including commonly consumed carbohydrates like glucose, fructose, sucrose, and lactose. The evaporative light-scattering detector (ELSD) is utilized as an alternative to an MS detector, and the separation system's selectivity allows the separation of anomers of monosaccharides. The study also includes a rapid method for determining isomaltulose (Palatinose), which was validated and applied to food supplement samples available in the Czech market, even those with high protein content. Additionally, isomaltulose was separated from sucrose in just 13 min.

Links

MUNI/A/1236/2021, interní kód MU

Name: Stanovení cukerného profilu v mléku a speciální mléčné výživě pomocí HPLC

Investor: Masaryk University

Citovat

CRHA, Tomáš, Grace F ODEDINA and Jiří PAZOUREK. HILIC Separation Methods on Poly-Hydroxyl Stationary Phases for Determination of Common Saccharides with Evaporative Light-Scattering Detector and Rapid Determination of Isomaltulose in Protein-Rich Food Supplements. Fluid - Particle Separations Journal. BASEL: American Filtration and Separations Society, 2024, vol. 11, No 2, p. 1-16. ISSN 1541-9290. Available from: https://dx.doi.org/10.3390/separations11020045.

@article{2388080,
   author = {Crha, Tomáš and Odedina, Grace F and Pazourek, Jiří},
   article_location = {BASEL},
   article_number = {2},
   doi = {http://dx.doi.org/10.3390/separations11020045},
   keywords = {HILIC; ELSD; saccharides; sugar alcohols; isomaltulose (Palatinose); food supplements},
   language = {eng},
   issn = {1541-9290},
   journal = {Fluid - Particle Separations Journal},
   title = {HILIC Separation Methods on Poly-Hydroxyl Stationary Phases for Determination of Common Saccharides with Evaporative Light-Scattering Detector and Rapid Determination of Isomaltulose in Protein-Rich Food Supplements},
   url = {https://www.mdpi.com/2297-8739/11/2/45},
   volume = {11},
   year = {2024}
}

TY  - JOUR
ID  - 2388080
AU  - Crha, Tomáš - Odedina, Grace F - Pazourek, Jiří
PY  - 2024
TI  - HILIC Separation Methods on Poly-Hydroxyl Stationary Phases for Determination of Common Saccharides with Evaporative Light-Scattering Detector and Rapid Determination of Isomaltulose in Protein-Rich Food Supplements
JF  - Fluid - Particle Separations Journal
VL  - 11
IS  - 2
SP  - 1-16
EP  - 1-16
PB  - American Filtration and Separations Society
SN  - 15419290
KW  - HILIC
KW  - ELSD
KW  - saccharides
KW  - sugar alcohols
KW  - isomaltulose (Palatinose)
KW  - food supplements
UR  - https://www.mdpi.com/2297-8739/11/2/45
N2  - This article highlights the fundamental aspects of hydrophilic interaction liquid chromatography (HILIC) on poly-hydroxyl stationary phases to analyze non-derivatized mono- and disaccharides, including commonly consumed carbohydrates like glucose, fructose, sucrose, and lactose. The evaporative light-scattering detector (ELSD) is utilized as an alternative to an MS detector, and the separation system's selectivity allows the separation of anomers of monosaccharides. The study also includes a rapid method for determining isomaltulose (Palatinose), which was validated and applied to food supplement samples available in the Czech market, even those with high protein content. Additionally, isomaltulose was separated from sucrose in just 13 min.
ER  -

CRHA, Tomáš, Grace F ODEDINA and Jiří PAZOUREK. HILIC Separation Methods on Poly-Hydroxyl Stationary Phases for Determination of Common Saccharides with Evaporative Light-Scattering Detector and Rapid Determination of Isomaltulose in Protein-Rich Food Supplements. \textit{Fluid - Particle Separations Journal}. BASEL: American Filtration and Separations Society, 2024, vol.~11, No~2, p.~1-16. ISSN~1541-9290. Available from: https://dx.doi.org/10.3390/separations11020045.

Detailed Information on Publication Record