HILIC Separation Methods on Poly-Hydroxyl Stationary Phases for Determination of Common Saccharides with Evaporative Light-Scattering Detector and Rapid Determination of Isomaltulose in Protein-Rich Food Supplements

CRHA, Tomas, Grace F ODEDINA and Jiří PAZOUREK. HILIC Separation Methods on Poly-Hydroxyl Stationary Phases for Determination of Common Saccharides with Evaporative Light-Scattering Detector and Rapid Determination of Isomaltulose in Protein-Rich Food Supplements. SEPARATIONS. SWITZERLAND: MDPI, 2024, vol. 11, No 2, 16 pp. ISSN 2297-8739. Available from: https://dx.doi.org/10.3390/separations11020045.

Basic information

• Original name: HILIC Separation Methods on Poly-Hydroxyl Stationary Phases for Determination of Common Saccharides with Evaporative Light-Scattering Detector and Rapid Determination of Isomaltulose in Protein-Rich Food Supplements
• Name in Czech: HILIC separační metody na polyhydroxylových stacionárních fázích pro stanovení běžných sacharidů s výparným detektorem rozptylu světla a rychlé stanovení isomaltulózy v potravinových doplňcích bohatých na bílkoviny
• Authors: CRHA, Tomas, Grace F ODEDINA and Jiří PAZOUREK.
• Edition: SEPARATIONS, SWITZERLAND, MDPI, 2024, 2297-8739.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Country of publisher: Switzerland
• Confidentiality degree: is not subject to a state or trade secret
• Impact factor: Impact factor: 2.600 in 2022
• Organization unit: Faculty of Pharmacy
• Doi: http://dx.doi.org/10.3390/separations11020045
• UT WoS: 001172378500001
• Keywords (in Czech): HILIC; ELSD; sacharidy; cukerné alkoholy; isomaltulosa (Palatinosa); potravinové doplňky
• Keywords in English: HILIC; ELSD; saccharides; sugar alcohols; isomaltulose (Palatinose); food supplements

Abstract

This article highlights the fundamental aspects of hydrophilic interaction liquid chromatography (HILIC) on poly-hydroxyl stationary phases to analyze non-derivatized mono- and disaccharides, including commonly consumed carbohydrates like glucose, fructose, sucrose, and lactose. The evaporative light-scattering detector (ELSD) is utilized as an alternative to an MS detector, and the separation system's selectivity allows the separation of anomers of monosaccharides. The study also includes a rapid method for determining isomaltulose (Palatinose), which was validated and applied to food supplement samples available in the Czech market, even those with high protein content. Additionally, isomaltulose was separated from sucrose in just 13 min.