Detailed Information on Publication Record
2024
Synthetically-primed adaptation of Pseudomonas putida to a non-native substrate D-xylose
DVOŘÁK, Pavel, Barbora BURÝŠKOVÁ, Barbora POPELÁŘOVÁ, Birgitta E. EBERT, Tibor BOTKA et. al.Basic information
Original name
Synthetically-primed adaptation of Pseudomonas putida to a non-native substrate D-xylose
Authors
DVOŘÁK, Pavel (203 Czech Republic, guarantor, belonging to the institution), Barbora BURÝŠKOVÁ (203 Czech Republic, belonging to the institution), Barbora POPELÁŘOVÁ (203 Czech Republic, belonging to the institution), Birgitta E. EBERT, Tibor BOTKA (203 Czech Republic, belonging to the institution), Dalimil BUJDOŠ, Alberto SÁNCHEZ-PASCUALA, Hannah SCHÖTTLER, Heiko HAYEN, Víctor DE LORENZO, Lars M. BLANK and Martin BENEŠÍK (203 Czech Republic, belonging to the institution)
Edition
Nature Communications, Nature Portfolio, 2024, 2041-1723
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
20801 Environmental biotechnology
Country of publisher
Germany
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 16.600 in 2022
Organization unit
Faculty of Science
UT WoS
001191874200006
Keywords in English
Applied microbiology; Bacterial evolution; Experimental evolution; Metabolic engineering
Tags
International impact, Reviewed
Změněno: 14/6/2024 11:22, Mgr. Marie Šípková, DiS.
Abstract
V originále
To broaden the substrate scope of microbial cell factories towards renewable substrates, rational genetic interventions are often combined with adaptive laboratory evolution (ALE). However, comprehensive studies enabling a holistic understanding of adaptation processes primed by rational metabolic engineering remain scarce. The industrial workhorse Pseudomonas putida was engineered to utilize the non-native sugar D-xylose, but its assimilation into the bacterial biochemical network via the exogenous xylose isomerase pathway remained unresolved. Here, we elucidate the xylose metabolism and establish a foundation for further engineering followed by ALE. First, native glycolysis is derepressed by deleting the local transcriptional regulator gene hexR. We then enhance the pentose phosphate pathway by implanting exogenous transketolase and transaldolase into two lag-shortened strains and allow ALE to finetune the rewired metabolism. Subsequent multilevel analysis and reverse engineering provide detailed insights into the parallel paths of bacterial adaptation to the non-native carbon source, highlighting the enhanced expression of transaldolase and xylose isomerase along with derepressed glycolysis as key events during the process.
Links
GA22-12505S, research and development project |
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LM2023042, research and development project |
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LX22NPO5103, research and development project |
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MUNI/J/0003/2021, interní kód MU |
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90254, large research infrastructures |
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