| DVOŘÁK, Pavel, Barbora BURÝŠKOVÁ, Barbora POPELÁŘOVÁ, Birgitta E. EBERT, Tibor BOTKA, Dalimil BUJDOŠ, Alberto SÁNCHEZ-PASCUALA, Hannah SCHÖTTLER, Heiko HAYEN, Víctor DE LORENZO, Lars M. BLANK and Martin BENEŠÍK. Synthetically-primed adaptation of Pseudomonas putida to a non-native substrate D-xylose. Nature Communications. Nature Portfolio, 2024, vol. 15, March 2024, p. 1-18. ISSN 2041-1723. Available from: https://dx.doi.org/10.1038/s41467-024-46812-9. |
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@article{2388277,
author = {Dvořák, Pavel and Burýšková, Barbora and Popelářová, Barbora and Ebert, Birgitta E. and Botka, Tibor and Bujdoš, Dalimil and SánchezandPascuala, Alberto and Schöttler, Hannah and Hayen, Heiko and de Lorenzo, Víctor and Blank, Lars M. and Benešík, Martin},
article_number = {March 2024},
doi = {http://dx.doi.org/10.1038/s41467-024-46812-9},
keywords = {Applied microbiology; Bacterial evolution; Experimental evolution; Metabolic engineering},
language = {eng},
issn = {2041-1723},
journal = {Nature Communications},
title = {Synthetically-primed adaptation of Pseudomonas putida to a non-native substrate D-xylose},
url = {https://www.nature.com/articles/s41467-024-46812-9},
volume = {15},
year = {2024}
}
TY - JOUR
ID - 2388277
AU - Dvořák, Pavel - Burýšková, Barbora - Popelářová, Barbora - Ebert, Birgitta E. - Botka, Tibor - Bujdoš, Dalimil - Sánchez-Pascuala, Alberto - Schöttler, Hannah - Hayen, Heiko - de Lorenzo, Víctor - Blank, Lars M. - Benešík, Martin
PY - 2024
TI - Synthetically-primed adaptation of Pseudomonas putida to a non-native substrate D-xylose
JF - Nature Communications
VL - 15
IS - March 2024
SP - 1-18
EP - 1-18
PB - Nature Portfolio
SN - 20411723
KW - Applied microbiology
KW - Bacterial evolution
KW - Experimental evolution
KW - Metabolic engineering
UR - https://www.nature.com/articles/s41467-024-46812-9
N2 - To broaden the substrate scope of microbial cell factories towards renewable substrates, rational genetic interventions are often combined with adaptive laboratory evolution (ALE). However, comprehensive studies enabling a holistic understanding of adaptation processes primed by rational metabolic engineering remain scarce. The industrial workhorse Pseudomonas putida was engineered to utilize the non-native sugar D-xylose, but its assimilation into the bacterial biochemical network via the exogenous xylose isomerase pathway remained unresolved. Here, we elucidate the xylose metabolism and establish a foundation for further engineering followed by ALE. First, native glycolysis is derepressed by deleting the local transcriptional regulator gene hexR. We then enhance the pentose phosphate pathway by implanting exogenous transketolase and transaldolase into two lag-shortened strains and allow ALE to finetune the rewired metabolism. Subsequent multilevel analysis and reverse engineering provide detailed insights into the parallel paths of bacterial adaptation to the non-native carbon source, highlighting the enhanced expression of transaldolase and xylose isomerase along with derepressed glycolysis as key events during the process.
ER -
DVOŘÁK, Pavel, Barbora BURÝŠKOVÁ, Barbora POPELÁŘOVÁ, Birgitta E. EBERT, Tibor BOTKA, Dalimil BUJDOŠ, Alberto SÁNCHEZ-PASCUALA, Hannah SCHÖTTLER, Heiko HAYEN, Víctor DE LORENZO, Lars M. BLANK and Martin BENEŠÍK. Synthetically-primed adaptation of Pseudomonas putida to a non-native substrate D-xylose. \textit{Nature Communications}. Nature Portfolio, 2024, vol.~15, March 2024, p.~1-18. ISSN~2041-1723. Available from: https://dx.doi.org/10.1038/s41467-024-46812-9.
|
