Photoelectrochemical Enzyme Biosensor for Malate Using Quantum Dots on Indium Tin Oxide/Plastics as a Sensing Surface

HLAVÁČOVÁ, Tereza and Petr SKLÁDAL. Photoelectrochemical Enzyme Biosensor for Malate Using Quantum Dots on Indium Tin Oxide/Plastics as a Sensing Surface. Biosensors. MDPI, 2024, vol. 14, No 1, p. 1-13. ISSN 2079-6374. Available from: https://dx.doi.org/10.3390/bios14010011.

Basic information

• Original name: Photoelectrochemical Enzyme Biosensor for Malate Using Quantum Dots on Indium Tin Oxide/Plastics as a Sensing Surface
• Authors: HLAVÁČOVÁ, Tereza (203 Czech Republic, belonging to the institution) and Petr SKLÁDAL (203 Czech Republic, guarantor, belonging to the institution).
• Edition: Biosensors, MDPI, 2024, 2079-6374.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10608 Biochemistry and molecular biology
• Country of publisher: Switzerland
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 5.400 in 2022
• Organization unit: Faculty of Science
• Doi: http://dx.doi.org/10.3390/bios14010011
• UT WoS: 001149456100001
• Keywords in English: malate dehydrogenase; flow-through biosensor; electrochemical sensor; wine; fruit juice
• Tags: rivok
• Tags: International impact, Reviewed

Abstract

A photoelectrochemical biosensor for malate was developed using an indium tin oxide (ITO) layer deposited on a poly(ethylene terephthalate) plastic sheet as a transparent electrode material for the immobilization of malate dehydrogenase together with CdTe quantum dots. Different approaches were compared for the construction of the bioactive layer; the highest response was achieved by depositing malate dehydrogenase together with CdTe nanoparticles and covering it with a Nafion/water (1:1) mixture. The amperometric signal of this biosensor was recorded during irradiation with a near-UV LED in the flow-through mode. The limit of detection was 0.28 mmol/L, which is adequate for analyzing malic acid levels in drinks such as white wines and fruit juices. The results confirm that the cheap ITO layer deposited on the plastic sheet after cutting into rectangular electrodes allows for the economic production of photoelectrochemical (bio)sensors. The combination of NAD+-dependent malate dehydrogenase with quantum dots was also compatible with such an ITO surface.

Links

• MUNI/A/1313/2022, interní kód MU: Name: Podpora biochemického výzkumu v roce 2023

Investor: Masaryk University