Photoelectrochemical Enzyme Biosensor for Malate Using Quantum
Dots on Indium Tin Oxide/Plastics as a Sensing Surface

J 2024

Photoelectrochemical Enzyme Biosensor for Malate Using Quantum Dots on Indium Tin Oxide/Plastics as a Sensing Surface

HLAVÁČOVÁ, Tereza and Petr SKLÁDAL

Basic information

Original name

Photoelectrochemical Enzyme Biosensor for Malate Using Quantum Dots on Indium Tin Oxide/Plastics as a Sensing Surface

Authors

HLAVÁČOVÁ, Tereza (203 Czech Republic, belonging to the institution) and Petr SKLÁDAL (203 Czech Republic, guarantor, belonging to the institution)

Edition

Biosensors, MDPI, 2024, 2079-6374

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.400 in 2022

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.3390/bios14010011

UT WoS

001149456100001

Keywords in English

malate dehydrogenase; flow-through biosensor; electrochemical sensor; wine; fruit juice

Abstract

V originále

A photoelectrochemical biosensor for malate was developed using an indium tin oxide (ITO) layer deposited on a poly(ethylene terephthalate) plastic sheet as a transparent electrode material for the immobilization of malate dehydrogenase together with CdTe quantum dots. Different approaches were compared for the construction of the bioactive layer; the highest response was achieved by depositing malate dehydrogenase together with CdTe nanoparticles and covering it with a Nafion/water (1:1) mixture. The amperometric signal of this biosensor was recorded during irradiation with a near-UV LED in the flow-through mode. The limit of detection was 0.28 mmol/L, which is adequate for analyzing malic acid levels in drinks such as white wines and fruit juices. The results confirm that the cheap ITO layer deposited on the plastic sheet after cutting into rectangular electrodes allows for the economic production of photoelectrochemical (bio)sensors. The combination of NAD+-dependent malate dehydrogenase with quantum dots was also compatible with such an ITO surface.

Links

MUNI/A/1313/2022, interní kód MU

Name: Podpora biochemického výzkumu v roce 2023

Investor: Masaryk University

Citovat

HLAVÁČOVÁ, Tereza and Petr SKLÁDAL. Photoelectrochemical Enzyme Biosensor for Malate Using Quantum Dots on Indium Tin Oxide/Plastics as a Sensing Surface. Biosensors. MDPI, 2024, vol. 14, No 1, p. 1-13. ISSN 2079-6374. Available from: https://dx.doi.org/10.3390/bios14010011.

@article{2393097,
   author = {Hlaváčová, Tereza and Skládal, Petr},
   article_number = {1},
   doi = {http://dx.doi.org/10.3390/bios14010011},
   keywords = {malate dehydrogenase; flow-through biosensor; electrochemical sensor; wine; fruit juice},
   language = {eng},
   issn = {2079-6374},
   journal = {Biosensors},
   title = {Photoelectrochemical Enzyme Biosensor for Malate Using Quantum Dots on Indium Tin Oxide/Plastics as a Sensing Surface},
   url = {https://www.mdpi.com/2079-6374/14/1/11},
   volume = {14},
   year = {2024}
}

TY  - JOUR
ID  - 2393097
AU  - Hlaváčová, Tereza - Skládal, Petr
PY  - 2024
TI  - Photoelectrochemical Enzyme Biosensor for Malate Using Quantum Dots on Indium Tin Oxide/Plastics as a Sensing Surface
JF  - Biosensors
VL  - 14
IS  - 1
SP  - 1-13
EP  - 1-13
PB  - MDPI
SN  - 20796374
KW  - malate dehydrogenase
KW  - flow-through biosensor
KW  - electrochemical sensor
KW  - wine
KW  - fruit juice
UR  - https://www.mdpi.com/2079-6374/14/1/11
N2  - A photoelectrochemical biosensor for malate was developed using an indium tin oxide (ITO) layer deposited on a poly(ethylene terephthalate) plastic sheet as a transparent electrode material for the immobilization of malate dehydrogenase together with CdTe quantum dots. Different approaches were compared for the construction of the bioactive layer; the highest response was achieved by depositing malate dehydrogenase together with CdTe nanoparticles and covering it with a Nafion/water (1:1) mixture. The amperometric signal of this biosensor was recorded during irradiation with a near-UV LED in the flow-through mode. The limit of detection was 0.28 mmol/L, which is adequate for analyzing malic acid levels in drinks such as white wines and fruit juices. The results confirm that the cheap ITO layer deposited on the plastic sheet after cutting into rectangular electrodes allows for the economic production of photoelectrochemical (bio)sensors. The combination of NAD+-dependent malate dehydrogenase with quantum dots was also compatible with such an ITO surface.
ER  -

HLAVÁČOVÁ, Tereza and Petr SKLÁDAL. Photoelectrochemical Enzyme Biosensor for Malate Using Quantum Dots on Indium Tin Oxide/Plastics as a Sensing Surface. \textit{Biosensors}. MDPI, 2024, vol.~14, No~1, p.~1-13. ISSN~2079-6374. Available from: https://dx.doi.org/10.3390/bios14010011.

Detailed Information on Publication Record