Brillouin light scattering anisotropy microscopy for imaging
the viscoelastic anisotropy in living cells

J 2024

Brillouin light scattering anisotropy microscopy for imaging the viscoelastic anisotropy in living cells

KESHMIRI, Hamid, Domagoj CIKES, Markéta ŠÁMALOVÁ, Lukas SCHINDLER, Lisa-Marie APPEL et. al.

Basic information

Original name

Brillouin light scattering anisotropy microscopy for imaging the viscoelastic anisotropy in living cells

Authors

KESHMIRI, Hamid, Domagoj CIKES, Markéta ŠÁMALOVÁ (203 Czech Republic, belonging to the institution), Lukas SCHINDLER, Lisa-Marie APPEL, Michal URBANEK, Ivan YUDUSHKIN, Dea SLADE, Wolfgang J WENINGER, Alexis PEAUCELLE, Josef PENNINGER and Kareem ELSAYAD

Edition

Nature Photonics, Nature Research, 2024, 1749-4885

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10610 Biophysics

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 35.000 in 2022

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1038/s41566-023-01368-w

UT WoS

001145338600002

Keywords in English

biophysics; optical spectroscopy; mechanical forces; growth; symmetry; reveals

Abstract

V originále

Maintaining and modulating mechanical anisotropy is essential for biological processes. However, how this is achieved at the microscopic scale in living soft matter is not always clear. Although Brillouin light scattering (BLS) spectroscopy can probe the mechanical properties of materials, spatiotemporal mapping of mechanical anisotropies in living matter with BLS microscopy has been complicated by the need for sequential measurements with tilted excitation and detection angles. Here we introduce Brillouin light scattering anisotropy microscopy (BLAM) for mapping high-frequency viscoelastic anisotropy inside living cells. BLAM employs a radial virtually imaged phased array that enables the collection of angle-resolved dispersion in a single shot, thus enabling us to probe phonon modes in living matter along different directions simultaneously. We demonstrate a precision of 10 MHz in the determination of the Brillouin frequency shift, at a spatial resolution of 2 mu m. Following proof-of-principle experiments on muscle myofibres, we apply BLAM to the study of two fundamental biological processes. In plant cell walls, we observe a switch from anisotropic to isotropic wall properties that may lead to asymmetric growth. In mammalian cell nuclei, we uncover a spatiotemporally oscillating elastic anisotropy correlated to chromatin condensation. Our results highlight the role that high-frequency mechanics can play in the regulation of diverse fundamental processes in biological systems. We expect BLAM to find diverse applications in biomedical imaging and material characterization.

Links

90251, large research infrastructures

Name: CzechNanoLab II

Citovat

KESHMIRI, Hamid, Domagoj CIKES, Markéta ŠÁMALOVÁ, Lukas SCHINDLER, Lisa-Marie APPEL, Michal URBANEK, Ivan YUDUSHKIN, Dea SLADE, Wolfgang J WENINGER, Alexis PEAUCELLE, Josef PENNINGER and Kareem ELSAYAD. Brillouin light scattering anisotropy microscopy for imaging the viscoelastic anisotropy in living cells. Nature Photonics. Nature Research, 2024, vol. 18, No 3, p. 1-13. ISSN 1749-4885. Available from: https://dx.doi.org/10.1038/s41566-023-01368-w.

@article{2393101,
   author = {Keshmiri, Hamid and Cikes, Domagoj and Šámalová, Markéta and Schindler, Lukas and Appel, LisaandMarie and Urbanek, Michal and Yudushkin, Ivan and Slade, Dea and Weninger, Wolfgang J and Peaucelle, Alexis and Penninger, Josef and Elsayad, Kareem},
   article_number = {3},
   doi = {http://dx.doi.org/10.1038/s41566-023-01368-w},
   keywords = {biophysics; optical spectroscopy; mechanical forces; growth; symmetry; reveals},
   language = {eng},
   issn = {1749-4885},
   journal = {Nature Photonics},
   title = {Brillouin light scattering anisotropy microscopy for imaging the viscoelastic anisotropy in living cells},
   url = {https://www.nature.com/articles/s41566-023-01368-w},
   volume = {18},
   year = {2024}
}

TY  - JOUR
ID  - 2393101
AU  - Keshmiri, Hamid - Cikes, Domagoj - Šámalová, Markéta - Schindler, Lukas - Appel, Lisa-Marie - Urbanek, Michal - Yudushkin, Ivan - Slade, Dea - Weninger, Wolfgang J - Peaucelle, Alexis - Penninger, Josef - Elsayad, Kareem
PY  - 2024
TI  - Brillouin light scattering anisotropy microscopy for imaging the viscoelastic anisotropy in living cells
JF  - Nature Photonics
VL  - 18
IS  - 3
SP  - 1-13
EP  - 1-13
PB  - Nature Research
SN  - 17494885
KW  - biophysics
KW  - optical spectroscopy
KW  - mechanical forces
KW  - growth
KW  - symmetry
KW  - reveals
UR  - https://www.nature.com/articles/s41566-023-01368-w
N2  - Maintaining and modulating mechanical anisotropy is essential for biological processes. However, how this is achieved at the microscopic scale in living soft matter is not always clear. Although Brillouin light scattering (BLS) spectroscopy can probe the mechanical properties of materials, spatiotemporal mapping of mechanical anisotropies in living matter with BLS microscopy has been complicated by the need for sequential measurements with tilted excitation and detection angles. Here we introduce Brillouin light scattering anisotropy microscopy (BLAM) for mapping high-frequency viscoelastic anisotropy inside living cells. BLAM employs a radial virtually imaged phased array that enables the collection of angle-resolved dispersion in a single shot, thus enabling us to probe phonon modes in living matter along different directions simultaneously. We demonstrate a precision of 10 MHz in the determination of the Brillouin frequency shift, at a spatial resolution of 2 mu m. Following proof-of-principle experiments on muscle myofibres, we apply BLAM to the study of two fundamental biological processes. In plant cell walls, we observe a switch from anisotropic to isotropic wall properties that may lead to asymmetric growth. In mammalian cell nuclei, we uncover a spatiotemporally oscillating elastic anisotropy correlated to chromatin condensation. Our results highlight the role that high-frequency mechanics can play in the regulation of diverse fundamental processes in biological systems. We expect BLAM to find diverse applications in biomedical imaging and material characterization.
ER  -

KESHMIRI, Hamid, Domagoj CIKES, Markéta ŠÁMALOVÁ, Lukas SCHINDLER, Lisa-Marie APPEL, Michal URBANEK, Ivan YUDUSHKIN, Dea SLADE, Wolfgang J WENINGER, Alexis PEAUCELLE, Josef PENNINGER and Kareem ELSAYAD. Brillouin light scattering anisotropy microscopy for imaging the viscoelastic anisotropy in living cells. \textit{Nature Photonics}. Nature Research, 2024, vol.~18, No~3, p.~1-13. ISSN~1749-4885. Available from: https://dx.doi.org/10.1038/s41566-023-01368-w.

Detailed Information on Publication Record