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@proceedings{2393637,
author = {Macháčková, Táňa and Vychytilová, Petra and Boudná, Marie and Trachtová, Karolína and Pavlikova, Marketa and Kotoucek, Jan and Halámková, Jana and Al Tukmachi, Dagmar and Šána, Jiří and Pokorná, Petra and Šachlová, Milana and Slabý, Ondřej},
booktitle = {114th Annual Meeting of the American Association for Cancer Research (AACR)},
doi = {http://dx.doi.org/10.1158/1538-7445.AM2023-6709},
keywords = {colorectal cancer; biomarkers; non-coding RNAs; micro-RNA; small extracellular vesicles},
language = {eng},
title = {Utility of RNA sequencing for transcriptome analysis of small extracellular vesicles derived from blood sera of colorectal cancer patients},
url = {https://aacrjournals.org/cancerres/article/83/7_Supplement/6709/723464/Abstract-6709-Utility-of-RNA-sequencing-for},
year = {2023}
}
TY - CONF
ID - 2393637
AU - Macháčková, Táňa - Vychytilová, Petra - Boudná, Marie - Trachtová, Karolína - Pavlikova, Marketa - Kotoucek, Jan - Halámková, Jana - Al Tukmachi, Dagmar - Šána, Jiří - Pokorná, Petra - Šachlová, Milana - Slabý, Ondřej
PY - 2023
TI - Utility of RNA sequencing for transcriptome analysis of small extracellular vesicles derived from blood sera of colorectal cancer patients
KW - colorectal cancer
KW - biomarkers
KW - non-coding RNAs
KW - micro-RNA
KW - small extracellular vesicles
UR - https://aacrjournals.org/cancerres/article/83/7_Supplement/6709/723464/Abstract-6709-Utility-of-RNA-sequencing-for
N2 - Currently used molecular diagnostic tests for colorectal cancer (CRC) are underperforming and more sensitive, non-invasive biomarkers are needed. Long non-coding RNAs (lncRNA) and microRNA (miRNA) have shown potential as diagnostic biomarkers. Unfortunately, the identification of non-coding RNA circulating biomarkers in blood serum is significantly burdened by abundant RNA specimens from disrupted blood cells. Recently, small extracellular vesicles (sEVs) emerged as potential reservoirs of clinically relevant biomarkers, including lncRNAs and miRNAs. In theory, sEVs protect RNAs from degradation and might serve as a source of intact RNA for further analyses. However, there is a lack of evidence supporting the superior quality of RNA extracted from sEVs over RNA from whole blood serum. This study aimed to analyze the RNA content of blood serum and the sEVs derived from the blood serum of CRC patients and healthy controls using RNA sequencing. Moreover, small RNA sequencing was used to evaluate the difference in miRNA profiles of sEVs and corresponding blood sera of CRC patients and healthy controls.
ER -
MACHÁČKOVÁ, Táňa, Petra VYCHYTILOVÁ, Marie BOUDNÁ, Karolína TRACHTOVÁ, Marketa PAVLIKOVA, Jan KOTOUCEK, Jana HALÁMKOVÁ, Dagmar AL TUKMACHI, Jiří ŠÁNA, Petra POKORNÁ, Milana ŠACHLOVÁ and Ondřej SLABÝ. Utility of RNA sequencing for transcriptome analysis of small extracellular vesicles derived from blood sera of colorectal cancer patients. In \textit{114th Annual Meeting of the American Association for Cancer Research (AACR)}. 2023. Available from: https://dx.doi.org/10.1158/1538-7445.AM2023-6709.
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