Utility of RNA sequencing for transcriptome analysis of small
extracellular vesicles derived from blood sera of colorectal
cancer patients

a 2023

Utility of RNA sequencing for transcriptome analysis of small extracellular vesicles derived from blood sera of colorectal cancer patients

MACHÁČKOVÁ, Táňa, Petra VYCHYTILOVÁ, Marie BOUDNÁ, Karolína TRACHTOVÁ, Marketa PAVLIKOVA et. al.

Basic information

Original name

Utility of RNA sequencing for transcriptome analysis of small extracellular vesicles derived from blood sera of colorectal cancer patients

Authors

MACHÁČKOVÁ, Táňa (203 Czech Republic, belonging to the institution), Petra VYCHYTILOVÁ (203 Czech Republic, belonging to the institution), Marie BOUDNÁ (203 Czech Republic, belonging to the institution), Karolína TRACHTOVÁ (203 Czech Republic, belonging to the institution), Marketa PAVLIKOVA, Jan KOTOUCEK, Jana HALÁMKOVÁ (203 Czech Republic, belonging to the institution), Dagmar AL TUKMACHI (203 Czech Republic, belonging to the institution), Jiří ŠÁNA (203 Czech Republic, belonging to the institution), Petra POKORNÁ (203 Czech Republic, belonging to the institution), Milana ŠACHLOVÁ (203 Czech Republic, belonging to the institution) and Ondřej SLABÝ (203 Czech Republic, guarantor, belonging to the institution)

Edition

114th Annual Meeting of the American Association for Cancer Research (AACR), 2023

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

30204 Oncology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

RIV identification code

RIV/00216224:14740/23:00133957

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1158/1538-7445.AM2023-6709

UT WoS

001008499107019

Keywords in English

colorectal cancer; biomarkers; non-coding RNAs; micro-RNA; small extracellular vesicles

Abstract

V originále

Currently used molecular diagnostic tests for colorectal cancer (CRC) are underperforming and more sensitive, non-invasive biomarkers are needed. Long non-coding RNAs (lncRNA) and microRNA (miRNA) have shown potential as diagnostic biomarkers. Unfortunately, the identification of non-coding RNA circulating biomarkers in blood serum is significantly burdened by abundant RNA specimens from disrupted blood cells. Recently, small extracellular vesicles (sEVs) emerged as potential reservoirs of clinically relevant biomarkers, including lncRNAs and miRNAs. In theory, sEVs protect RNAs from degradation and might serve as a source of intact RNA for further analyses. However, there is a lack of evidence supporting the superior quality of RNA extracted from sEVs over RNA from whole blood serum. This study aimed to analyze the RNA content of blood serum and the sEVs derived from the blood serum of CRC patients and healthy controls using RNA sequencing. Moreover, small RNA sequencing was used to evaluate the difference in miRNA profiles of sEVs and corresponding blood sera of CRC patients and healthy controls.

Links

LM2018127, research and development project

Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)

Investor: Ministry of Education, Youth and Sports of the CR

LX22NPO5102, research and development project

Name: Národní ústav pro výzkum rakoviny (Acronym: NÚVR)

Investor: Ministry of Education, Youth and Sports of the CR, National institute for cancer research, 5.1 EXCELES

NU20-03-00127, research and development project

Name: Dlouhé nekódující RNA v exozómech jako biomarkery umožňující neinvazivní časnou detekci kolorektálního karcinomu

Investor: Ministry of Health of the CR, Exosomal long non-coding RNAs as non-invasive biomarkers for early detection of colorectal cancer, Subprogram 1 - standard

90242, large research infrastructures

Name: CIISB III

Citovat

MACHÁČKOVÁ, Táňa, Petra VYCHYTILOVÁ, Marie BOUDNÁ, Karolína TRACHTOVÁ, Marketa PAVLIKOVA, Jan KOTOUCEK, Jana HALÁMKOVÁ, Dagmar AL TUKMACHI, Jiří ŠÁNA, Petra POKORNÁ, Milana ŠACHLOVÁ and Ondřej SLABÝ. Utility of RNA sequencing for transcriptome analysis of small extracellular vesicles derived from blood sera of colorectal cancer patients. In 114th Annual Meeting of the American Association for Cancer Research (AACR). 2023. Available from: https://dx.doi.org/10.1158/1538-7445.AM2023-6709.

@proceedings{2393637,
   author = {Macháčková, Táňa and Vychytilová, Petra and Boudná, Marie and Trachtová, Karolína and Pavlikova, Marketa and Kotoucek, Jan and Halámková, Jana and Al Tukmachi, Dagmar and Šána, Jiří and Pokorná, Petra and Šachlová, Milana and Slabý, Ondřej},
   booktitle = {114th Annual Meeting of the American Association for Cancer Research (AACR)},
   doi = {http://dx.doi.org/10.1158/1538-7445.AM2023-6709},
   keywords = {colorectal cancer; biomarkers; non-coding RNAs; micro-RNA; small extracellular vesicles},
   language = {eng},
   title = {Utility of RNA sequencing for transcriptome analysis of small extracellular vesicles derived from blood sera of colorectal cancer patients},
   url = {https://aacrjournals.org/cancerres/article/83/7_Supplement/6709/723464/Abstract-6709-Utility-of-RNA-sequencing-for},
   year = {2023}
}

TY  - CONF
ID  - 2393637
AU  - Macháčková, Táňa - Vychytilová, Petra - Boudná, Marie - Trachtová, Karolína - Pavlikova, Marketa - Kotoucek, Jan - Halámková, Jana - Al Tukmachi, Dagmar - Šána, Jiří - Pokorná, Petra - Šachlová, Milana - Slabý, Ondřej
PY  - 2023
TI  - Utility of RNA sequencing for transcriptome analysis of small extracellular vesicles derived from blood sera of colorectal cancer patients
KW  - colorectal cancer
KW  - biomarkers
KW  - non-coding RNAs
KW  - micro-RNA
KW  - small extracellular vesicles
UR  - https://aacrjournals.org/cancerres/article/83/7_Supplement/6709/723464/Abstract-6709-Utility-of-RNA-sequencing-for
N2  - Currently used molecular diagnostic tests for colorectal cancer (CRC) are underperforming and more sensitive, non-invasive biomarkers are needed. Long non-coding RNAs (lncRNA) and microRNA (miRNA) have shown potential as diagnostic biomarkers. Unfortunately, the identification of non-coding RNA circulating biomarkers in blood serum is significantly burdened by abundant RNA specimens from disrupted blood cells. Recently, small extracellular vesicles (sEVs) emerged as potential reservoirs of clinically relevant biomarkers, including lncRNAs and miRNAs. In theory, sEVs protect RNAs from degradation and might serve as a source of intact RNA for further analyses. However, there is a lack of evidence supporting the superior quality of RNA extracted from sEVs over RNA from whole blood serum. This study aimed to analyze the RNA content of blood serum and the sEVs derived from the blood serum of CRC patients and healthy controls using RNA sequencing. Moreover, small RNA sequencing was used to evaluate the difference in miRNA profiles of sEVs and corresponding blood sera of CRC patients and healthy controls.
ER  -

MACHÁČKOVÁ, Táňa, Petra VYCHYTILOVÁ, Marie BOUDNÁ, Karolína TRACHTOVÁ, Marketa PAVLIKOVA, Jan KOTOUCEK, Jana HALÁMKOVÁ, Dagmar AL TUKMACHI, Jiří ŠÁNA, Petra POKORNÁ, Milana ŠACHLOVÁ and Ondřej SLABÝ. Utility of RNA sequencing for transcriptome analysis of small extracellular vesicles derived from blood sera of colorectal cancer patients. In \textit{114th Annual Meeting of the American Association for Cancer Research (AACR)}. 2023. Available from: https://dx.doi.org/10.1158/1538-7445.AM2023-6709.

Detailed Information on Publication Record