Detailed Information on Publication Record
2000
Determination of pyrroloquinoline quinone by capillary zone electrophoresis
GLATZ, Zdeněk, Martina MORAVCOVÁ and Oldřich JANICZEKBasic information
Original name
Determination of pyrroloquinoline quinone by capillary zone electrophoresis
Authors
GLATZ, Zdeněk, Martina MORAVCOVÁ and Oldřich JANICZEK
Edition
Amsterdam, 7 pp. Journal of Chromatography B, 739/1, 101 - 107, 2000
Publisher
Elsevier
Other information
Language
English
Type of outcome
Odborná kniha
Field of Study
10600 1.6 Biological sciences
Country of publisher
Netherlands
Confidentiality degree
není předmětem státního či obchodního tajemství
RIV identification code
RIV/00216224:14310/00:00003262
Organization unit
Faculty of Science
ISBN
ISSN 0378-4347
UT WoS
000085807600010
Keywords in English
capillary zone electrophoresis; PQQ; pyrroloquinoline quinone; quinoproteins
Změněno: 28/2/2001 09:50, prof. RNDr. Zdeněk Glatz, CSc.
Abstract
V originále
A new method for the determination of pyrroloquinoline quinone by capillary zone electrophoresis has been developed. Separation conditions have been optimised with the respect to different parameters including pH and ionic strength of the background electrolyte, separation voltage and temperature of the capillary. A buffer consisting of 50 mM b-alanine - HCl pH 3.0 was found to be the most suitable electrolyte for this separation. An applied voltage of 25 kV (negative polarity) and a temperature of 25 oC gave the best analysis of pyrroloquinoline quinone. The linear detection range for concentration versus peak area for the assay is from 5 to 500 mM (correlation coefficient 0.9998) with a detection limit of 0.1- 0.2 mM. The inter-day reproducibility of the peak area was 2.5 % and the inter-day reproducibility of the migration time was below 0.18 %.
Links
| GA525/97/0340, research and development project |
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