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HOFMANOVÁ, Jiřina, Josef SLAVIK, Miroslav CIGANEK, Petra OVESNÁ, Zuzana TYLICHOVA, Martina KARASOVÁ, Ondrej ZAPLETAL, Nicol STRAKOVA, Jiřina PROCHÁZKOVÁ, Jan BOUCHAL, Zdenek KOLAR, Jiri EHRMANN, Monika LEVKOVA, Zlatka HUSKOVA, Pavel SKALICKY, Alois KOZUBÍK, Miroslav MACHALA and Jan VONDRÁČEK. Complex Alterations of Fatty Acid Metabolism and Phospholipidome Uncovered in Isolated Colon Cancer Epithelial Cells. International Journal of Molecular Sciences. Basel: Multidisciplinary Digital Publishing Institute, 2021, vol. 22, No 13, p. 1-16. ISSN 1422-0067. Available from: https://dx.doi.org/10.3390/ijms22136650.

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TY  - JOUR
ID  - 2396648
AU  - Hofmanová, Jiřina - Slavik, Josef - Ciganek, Miroslav - Ovesná, Petra - Tylichova, Zuzana - Karasová, Martina - Zapletal, Ondrej - Strakova, Nicol - Procházková, Jiřina - Bouchal, Jan - Kolar, Zdenek - Ehrmann, Jiri - Levkova, Monika - Huskova, Zlatka - Skalicky, Pavel - Kozubík, Alois - Machala, Miroslav - Vondráček, Jan
PY  - 2021
TI  - Complex Alterations of Fatty Acid Metabolism and Phospholipidome Uncovered in Isolated Colon Cancer Epithelial Cells
JF  - International Journal of Molecular Sciences
VL  - 22
IS  - 13
SP  - 1-16
EP  - 1-16
PB  - Multidisciplinary Digital Publishing Institute
SN  - 14220067
KW  - phospholipids
KW  - lysophospholipids
KW  - fatty acid synthesis
KW  - desaturation
KW  - colorectal carcinoma
KW  - EpCAM
KW  - epithelial cells
KW  - lipidomics
UR  - https://www.mdpi.com/1422-0067/22/13/6650
N2  - The development of colon cancer, one of the most common malignancies, is accompanied with numerous lipid alterations. However, analyses of whole tumor samples may not always provide an accurate description of specific changes occurring directly in tumor epithelial cells. Here, we analyzed in detail the phospholipid (PL), lysophospholipid (lysoPL), and fatty acid (FA) profiles of purified EpCAM(+) cells, isolated from tumor and adjacent non-tumor tissues of colon cancer patients. We found that a number of FAs increased significantly in isolated tumor cells, which also included a number of long polyunsaturated FAs. Higher levels of FAs were associated with increased expression of FA synthesis genes, as well as with altered expression of enzymes involved in FA elongation and desaturation, including particularly fatty acid synthase, stearoyl-CoA desaturase, fatty acid desaturase 2 and ELOVL5 fatty acid elongase 5 We identified significant changes in ratios of specific lysoPLs and corresponding PLs. A number of lysophosphatidylcholine and lysophosphatidylethanolamine species, containing long-chain and very-long chain FAs, often with high numbers of double bonds, were significantly upregulated in tumor cells. Increased de novo synthesis of very long-chain FAs, or, altered uptake or incorporation of these FAs into specific lysoPLs in tumor cells, may thus contribute to reprogramming of cellular phospholipidome and membrane alterations observed in colon cancer.
ER  -

Basic information
Original name	Complex Alterations of Fatty Acid Metabolism and Phospholipidome Uncovered in Isolated Colon Cancer Epithelial Cells
Authors	HOFMANOVÁ, Jiřina, Josef SLAVIK, Miroslav CIGANEK, Petra OVESNÁ, Zuzana TYLICHOVA, Martina KARASOVÁ, Ondrej ZAPLETAL, Nicol STRAKOVA, Jiřina PROCHÁZKOVÁ, Jan BOUCHAL, Zdenek KOLAR, Jiri EHRMANN, Monika LEVKOVA, Zlatka HUSKOVA, Pavel SKALICKY, Alois KOZUBÍK, Miroslav MACHALA and Jan VONDRÁČEK.
Edition	International Journal of Molecular Sciences, Basel, Multidisciplinary Digital Publishing Institute, 2021, 1422-0067.

Other information
Original language	English
Type of outcome	Article in a journal
Field of Study	10608 Biochemistry and molecular biology
Country of publisher	Switzerland
Confidentiality degree	is not subject to a state or trade secret
WWW	URL
Impact factor	Impact factor: 6.208
Organization unit	Faculty of Science
Doi	http://dx.doi.org/10.3390/ijms22136650
UT WoS	000671202800001
Keywords in English	phospholipids; lysophospholipids; fatty acid synthesis; desaturation; colorectal carcinoma; EpCAM; epithelial cells; lipidomics
Tags	cancer, cellular plasticity, EMT, sphingolipids
Tags	International impact, Reviewed
Changed by	Changed by: Mgr. Jiřina Procházková, Ph.D., učo 43680. Changed: 24/4/2024 13:38.

Abstract
The development of colon cancer, one of the most common malignancies, is accompanied with numerous lipid alterations. However, analyses of whole tumor samples may not always provide an accurate description of specific changes occurring directly in tumor epithelial cells. Here, we analyzed in detail the phospholipid (PL), lysophospholipid (lysoPL), and fatty acid (FA) profiles of purified EpCAM(+) cells, isolated from tumor and adjacent non-tumor tissues of colon cancer patients. We found that a number of FAs increased significantly in isolated tumor cells, which also included a number of long polyunsaturated FAs. Higher levels of FAs were associated with increased expression of FA synthesis genes, as well as with altered expression of enzymes involved in FA elongation and desaturation, including particularly fatty acid synthase, stearoyl-CoA desaturase, fatty acid desaturase 2 and ELOVL5 fatty acid elongase 5 We identified significant changes in ratios of specific lysoPLs and corresponding PLs. A number of lysophosphatidylcholine and lysophosphatidylethanolamine species, containing long-chain and very-long chain FAs, often with high numbers of double bonds, were significantly upregulated in tumor cells. Increased de novo synthesis of very long-chain FAs, or, altered uptake or incorporation of these FAs into specific lysoPLs in tumor cells, may thus contribute to reprogramming of cellular phospholipidome and membrane alterations observed in colon cancer.

Abstract

The development of colon cancer, one of the most common malignancies, is accompanied with numerous lipid alterations. However, analyses of whole tumor samples may not always provide an accurate description of specific changes occurring directly in tumor epithelial cells. Here, we analyzed in detail the phospholipid (PL), lysophospholipid (lysoPL), and fatty acid (FA) profiles of purified EpCAM(+) cells, isolated from tumor and adjacent non-tumor tissues of colon cancer patients. We found that a number of FAs increased significantly in isolated tumor cells, which also included a number of long polyunsaturated FAs. Higher levels of FAs were associated with increased expression of FA synthesis genes, as well as with altered expression of enzymes involved in FA elongation and desaturation, including particularly fatty acid synthase, stearoyl-CoA desaturase, fatty acid desaturase 2 and ELOVL5 fatty acid elongase 5 We identified significant changes in ratios of specific lysoPLs and corresponding PLs. A number of lysophosphatidylcholine and lysophosphatidylethanolamine species, containing long-chain and very-long chain FAs, often with high numbers of double bonds, were significantly upregulated in tumor cells. Increased de novo synthesis of very long-chain FAs, or, altered uptake or incorporation of these FAs into specific lysoPLs in tumor cells, may thus contribute to reprogramming of cellular phospholipidome and membrane alterations observed in colon cancer.