Detailed Information on Publication Record
2024
Non canonical bases differentially represented in the sex chromosomes of the dioecious plant Silene latifolia
HUBINSKÝ, Marcel, Roman HOBZA, Marta STARCZAK, Daniel GACKOWSKI, Zdeněk KUBÁT et. al.Basic information
Original name
Non canonical bases differentially represented in the sex chromosomes of the dioecious plant Silene latifolia
Authors
HUBINSKÝ, Marcel (703 Slovakia, guarantor, belonging to the institution), Roman HOBZA (203 Czech Republic), Marta STARCZAK, Daniel GACKOWSKI, Zdeněk KUBÁT, Tomáš JANÍČEK, Lucie HORÁKOVÁ and José Luís RODRÍGUEZ LORENZO (724 Spain)
Edition
Journal of Experimental Botany, Oxford University Press, 2024, 0022-0957
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10611 Plant sciences, botany
Country of publisher
United Kingdom of Great Britain and Northern Ireland
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 6.900 in 2022
Organization unit
Faculty of Science
UT WoS
001243510300001
Keywords in English
Silene latifolia; sex chromosomes; transposable elements; cytosine modifications; dosage compensation; oxi-mCs
Tags
Tags
International impact, Reviewed
Změněno: 16/7/2024 09:21, Mgr. Marie Šípková, DiS.
Abstract
V originále
The oxidation of 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC), known as oxi-mCs, garners significant interest in plants as potential epigenetic marks. While research in mammals has established a role in cell reprogramming, carcinogenesis and gene regulation, their functions in plants remain unclear. In rice, 5hmC has been associated with transposable elements and heterochromatin. This study utilizes Silene latifolia, a dioecious plant with heteromorphic sex chromosomes and a genome with a large proportion of transposable elements, which provides a favourable environment for the study of oxi-mCs in individual sexes. Notably, we detected surprisingly high levels of oxi-mCs in S. latifolia comparable to mammals. Nuclei showed enrichment in heterochromatic regions, except for 5hmC which signal was homogeneously distributed. Intriguingly, the same X chromosome in females displayed overall enrichment of 5hmC and 5fC regarding its counterpart. This fact is shared with 5mC resembling dosage compensation. Colocalization showed higher correlation between 5mC and 5fC than with 5hmC, suggesting no potential relationship between 5hmC and 5fC. Additionally, the promoter of several sex-linked genes and sex biased TEs gathered on a clear sex-dependent clustering. Together, these findings unveil a hypothetical role of oxi-mCs in S. latifolia sex chromosome development, warranting further exploration.
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