J 2024

Glutathione S-transferase M1, T1, and P1 polymorphisms and periodontitis in a Caucasian population: a case-control study

IZAKOVIČOVÁ, Pavla, Antonín FASSMANN, Ladislav DUŠEK and Lydie IZAKOVIČOVÁ HOLLÁ

Basic information

Original name

Glutathione S-transferase M1, T1, and P1 polymorphisms and periodontitis in a Caucasian population: a case-control study

Authors

IZAKOVIČOVÁ, Pavla (203 Czech Republic, belonging to the institution), Antonín FASSMANN (203 Czech Republic, belonging to the institution), Ladislav DUŠEK (203 Czech Republic, belonging to the institution) and Lydie IZAKOVIČOVÁ HOLLÁ (203 Czech Republic, belonging to the institution)

Edition

BMC Oral Health, London, BMC, 2024, 1472-6831

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30208 Dentistry, oral surgery and medicine

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 2.900 in 2022

Organization unit

Faculty of Medicine

DOI

UT WoS

001177861200002

Keywords in English

Gene; Glutathione S-transferase; GSTs; Periodontitis; Polymorphism

Tags

Tags

International impact, Reviewed
Změněno: 3/5/2024 08:42, Mgr. Tereza Miškechová

Abstract

V originále

Background Glutathione S-transferases (GSTs) play important roles in protecting cells against oxidative stress and toxic chemicals. This study aimed to investigate the distribution of GSTM1, GSTT1, and GSTP1 variants and their roles in periodontitis susceptibility in a Caucasian population. Methods We analyzed 406 participants, including 204 healthy controls and 203 periodontitis patients. A multiplex polymerase chain reaction (PCR) approach was used to analyze GSTM1 and GSTT1 loci. GSTP1 variants were detected by PCR-haplotyping method in a subgroup of participants (N = 350). Chi-square or Fishers exact tests were used to compare genotypic and allelic differences. The Bonferroni method was applied to correct for multiple comparisons (p(corr)). Results The GSTM1 genotype distribution did not differ significantly between controls and periodontitis patients (p = 0.44). Additionally, the wild/null genotypes of GSTT1, Ile105Val and Ala114Val frequencies of GSTP1 were not significantly different between the two groups after correction for multiple comparisons (p = 0.05, p = 0.55, p = 0.02, p(corr)>0.05, respectively). The GSTM1 and GSTP1 Ile105Val gene variants were similarly distributed between non-smokers and smokers in both groups (p = 0.38, p = 0.20, and p = 0.14, p = 0.35, respectively). However, the wild genotype of the GSTT1 and Ala114Ala variant of the GSTP1 genes were present more frequently in non-smoking periodontitis patients than in non-smoking controls (p = 0.03, p(corr)>0.05, and p = 0.009, p(corr)>0.05, respectively) although their frequencies did not differ between smoking periodontitis patients and smoking controls (p = 0.23, p = 0.68, respectively). Conclusions This study in a Czech Caucasian population did not confirm a highly significant association between GST gene variants and susceptibility to periodontitis, as previously reported by Arshad and colleagues in Pakistanis. However, a weak relationship between GSTT1 and GSTP1 rs1138272 polymorphisms and periodontitis in non-smokers was observed.

Links

MUNI/A/1607/2023, interní kód MU
Name: Diagnostika a léčba vybraných chorob dutiny ústní
Investor: Masaryk University, Diagnosis and treatment of selected diseases of the oral cavity