Plectin plays a role in the migration and volume regulation of
astrocytes: a potential biomarker of glioblastoma

J 2024

Plectin plays a role in the migration and volume regulation of astrocytes: a potential biomarker of glioblastoma

ZUGEC, Maja, Borut FURLANI, Maria J CASTANON, Bostjan RITUPER, Irmgard FISCHER et. al.

Basic information

Original name

Plectin plays a role in the migration and volume regulation of astrocytes: a potential biomarker of glioblastoma

Authors

Edition

Journal of Biomedical Science, LONDON, BMC, 2024, 1021-7770

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 11.000 in 2022

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1186/s12929-024-01002-z

UT WoS

001147741700001

Keywords in English

Astrocyte; Glioblastoma; Plectin; Aquaporin 4; Intermediate filaments; Cytoskeleton; Edema; Cell volume; Cell migration

Abstract

V originále

BackgroundThe expression of aquaporin 4 (AQP4) and intermediate filament (IF) proteins is altered in malignant glioblastoma (GBM), yet the expression of the major IF-based cytolinker, plectin (PLEC), and its contribution to GBM migration and invasiveness, are unknown. Here, we assessed the contribution of plectin in affecting the distribution of plasmalemmal AQP4 aggregates, migratory properties, and regulation of cell volume in astrocytes.MethodsIn human GBM, the expression of glial fibrillary acidic protein (GFAP), AQP4 and PLEC transcripts was analyzed using publicly available datasets, and the colocalization of PLEC with AQP4 and with GFAP was determined by immunohistochemistry. We performed experiments on wild-type and plectin-deficient primary and immortalized mouse astrocytes, human astrocytes and permanent cell lines (U-251 MG and T98G) derived from a human malignant GBM. The expression of plectin isoforms in mouse astrocytes was assessed by quantitative real-time PCR. Transfection, immunolabeling and confocal microscopy were used to assess plectin-induced alterations in the distribution of the cytoskeleton, the influence of plectin and its isoforms on the abundance and size of plasmalemmal AQP4 aggregates, and the presence of plectin at the plasma membrane. The release of plectin from cells was measured by ELISA. The migration and dynamics of cell volume regulation of immortalized astrocytes were assessed by the wound-healing assay and calcein labeling, respectively.ResultsA positive correlation was found between plectin and AQP4 at the level of gene expression and protein localization in tumorous brain samples. Deficiency of plectin led to a decrease in the abundance and size of plasmalemmal AQP4 aggregates and altered distribution and bundling of the cytoskeleton. Astrocytes predominantly expressed P1c, P1e, and P1g plectin isoforms. The predominant plectin isoform associated with plasmalemmal AQP4 aggregates was P1c, which also affected the mobility of astrocytes most prominently. In the absence of plectin, the collective migration of astrocytes was impaired and the dynamics of cytoplasmic volume changes in peripheral cell regions decreased. Plectin's abundance on the plasma membrane surface and its release from cells were increased in the GBM cell lines.ConclusionsPlectin affects cellular properties that contribute to the pathology of GBM. The observed increase in both cell surface and released plectin levels represents a potential biomarker and therapeutic target in the diagnostics and treatment of GBMs.

Links

964997, interní kód MU

Name: Alliance for Life Sciences: From Strategies to Actions in Central and Eastern Europe (Acronym: A4L_ACTIONS)

Investor: European Union, Health, demographic change and wellbeing (Societal Challenges)

Citovat

ZUGEC, Maja, Borut FURLANI, Maria J CASTANON, Bostjan RITUPER, Irmgard FISCHER, Giuseppe BROGGI, Rosario CALTABIANO, Giuseppe M V BARBAGALLO, Di Rosa MICHELINO, Daniele TIBULLO, Rosalba PARENTI, Nunzio VICARIO, Sasa SIMCIC, Victorio Martin POZO DEVOTO, Gorazd B STOKIN, Gerhard WICHE, Jernej JORGACEVSKI, Robert ZOREC and Maja POTOKAR. Plectin plays a role in the migration and volume regulation of astrocytes: a potential biomarker of glioblastoma. Journal of Biomedical Science. LONDON: BMC, 2024, vol. 31, No 1, p. 1-22. ISSN 1021-7770. Available from: https://dx.doi.org/10.1186/s12929-024-01002-z.

@article{2398877,
   author = {Zugec, Maja and Furlani, Borut and Castanon, Maria J and Rituper, Bostjan and Fischer, Irmgard and Broggi, Giuseppe and Caltabiano, Rosario and Barbagallo, Giuseppe M V and Michelino, Di Rosa and Tibullo, Daniele and Parenti, Rosalba and Vicario, Nunzio and Simcic, Sasa and Pozo Devoto, Victorio Martin and Stokin, Gorazd B and Wiche, Gerhard and Jorgacevski, Jernej and Zorec, Robert and Potokar, Maja},
   article_location = {LONDON},
   article_number = {1},
   doi = {http://dx.doi.org/10.1186/s12929-024-01002-z},
   keywords = {Astrocyte; Glioblastoma; Plectin; Aquaporin 4; Intermediate filaments; Cytoskeleton; Edema; Cell volume; Cell migration},
   language = {eng},
   issn = {1021-7770},
   journal = {Journal of Biomedical Science},
   title = {Plectin plays a role in the migration and volume regulation of astrocytes: a potential biomarker of glioblastoma},
   url = {https://jbiomedsci.biomedcentral.com/articles/10.1186/s12929-024-01002-z},
   volume = {31},
   year = {2024}
}

TY  - JOUR
ID  - 2398877
AU  - Zugec, Maja - Furlani, Borut - Castanon, Maria J - Rituper, Bostjan - Fischer, Irmgard - Broggi, Giuseppe - Caltabiano, Rosario - Barbagallo, Giuseppe M V - Michelino, Di Rosa - Tibullo, Daniele - Parenti, Rosalba - Vicario, Nunzio - Simcic, Sasa - Pozo Devoto, Victorio Martin - Stokin, Gorazd B - Wiche, Gerhard - Jorgacevski, Jernej - Zorec, Robert - Potokar, Maja
PY  - 2024
TI  - Plectin plays a role in the migration and volume regulation of astrocytes: a potential biomarker of glioblastoma
JF  - Journal of Biomedical Science
VL  - 31
IS  - 1
SP  - 1-22
EP  - 1-22
PB  - BMC
SN  - 10217770
KW  - Astrocyte
KW  - Glioblastoma
KW  - Plectin
KW  - Aquaporin 4
KW  - Intermediate filaments
KW  - Cytoskeleton
KW  - Edema
KW  - Cell volume
KW  - Cell migration
UR  - https://jbiomedsci.biomedcentral.com/articles/10.1186/s12929-024-01002-z
N2  - BackgroundThe expression of aquaporin 4 (AQP4) and intermediate filament (IF) proteins is altered in malignant glioblastoma (GBM), yet the expression of the major IF-based cytolinker, plectin (PLEC), and its contribution to GBM migration and invasiveness, are unknown. Here, we assessed the contribution of plectin in affecting the distribution of plasmalemmal AQP4 aggregates, migratory properties, and regulation of cell volume in astrocytes.MethodsIn human GBM, the expression of glial fibrillary acidic protein (GFAP), AQP4 and PLEC transcripts was analyzed using publicly available datasets, and the colocalization of PLEC with AQP4 and with GFAP was determined by immunohistochemistry. We performed experiments on wild-type and plectin-deficient primary and immortalized mouse astrocytes, human astrocytes and permanent cell lines (U-251 MG and T98G) derived from a human malignant GBM. The expression of plectin isoforms in mouse astrocytes was assessed by quantitative real-time PCR. Transfection, immunolabeling and confocal microscopy were used to assess plectin-induced alterations in the distribution of the cytoskeleton, the influence of plectin and its isoforms on the abundance and size of plasmalemmal AQP4 aggregates, and the presence of plectin at the plasma membrane. The release of plectin from cells was measured by ELISA. The migration and dynamics of cell volume regulation of immortalized astrocytes were assessed by the wound-healing assay and calcein labeling, respectively.ResultsA positive correlation was found between plectin and AQP4 at the level of gene expression and protein localization in tumorous brain samples. Deficiency of plectin led to a decrease in the abundance and size of plasmalemmal AQP4 aggregates and altered distribution and bundling of the cytoskeleton. Astrocytes predominantly expressed P1c, P1e, and P1g plectin isoforms. The predominant plectin isoform associated with plasmalemmal AQP4 aggregates was P1c, which also affected the mobility of astrocytes most prominently. In the absence of plectin, the collective migration of astrocytes was impaired and the dynamics of cytoplasmic volume changes in peripheral cell regions decreased. Plectin's abundance on the plasma membrane surface and its release from cells were increased in the GBM cell lines.ConclusionsPlectin affects cellular properties that contribute to the pathology of GBM. The observed increase in both cell surface and released plectin levels represents a potential biomarker and therapeutic target in the diagnostics and treatment of GBMs.
ER  -

ZUGEC, Maja, Borut FURLANI, Maria J CASTANON, Bostjan RITUPER, Irmgard FISCHER, Giuseppe BROGGI, Rosario CALTABIANO, Giuseppe M V BARBAGALLO, Di Rosa MICHELINO, Daniele TIBULLO, Rosalba PARENTI, Nunzio VICARIO, Sasa SIMCIC, Victorio Martin POZO DEVOTO, Gorazd B STOKIN, Gerhard WICHE, Jernej JORGACEVSKI, Robert ZOREC and Maja POTOKAR. Plectin plays a role in the migration and volume regulation of astrocytes: a potential biomarker of glioblastoma. \textit{Journal of Biomedical Science}. LONDON: BMC, 2024, vol.~31, No~1, p.~1-22. ISSN~1021-7770. Available from: https://dx.doi.org/10.1186/s12929-024-01002-z.

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