Podrobný výpis o publikaci

MAURIZIO, Anna, Lucie ŠKORPÍKOVÁ, Jana ILGOVÁ, Cinzia TESSARIN, Giorgia DOTTO, Nikol RESLOVÁ, Jaroslav VADLEJCH, Erica MARCHIORI, di Regalbono Antonio FRANGIPANE, Martin KAŠNÝ a Rudi CASSINI. Faecal egg count reduction test in goats: Zooming in on the genus level. Veterinary Parasitology. AMSTERDAM: Elsevier Science, 2024, roč. 327, 8 s. ISSN 0304-4017. Dostupné z: https://dx.doi.org/10.1016/j.vetpar.2024.110146.

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TY  - JOUR
ID  - 2399418
AU  - Maurizio, Anna - Škorpíková, Lucie - Ilgová, Jana - Tessarin, Cinzia - Dotto, Giorgia - Reslová, Nikol - Vadlejch, Jaroslav - Marchiori, Erica - Frangipane, di Regalbono Antonio - Kašný, Martin - Cassini, Rudi
PY  - 2024
TI  - Faecal egg count reduction test in goats: Zooming in on the genus level
JF  - Veterinary Parasitology
VL  - 327
PB  - Elsevier Science
SN  - 03044017
KW  - Gastrointestinal tract
KW  - Caprine nematodes
KW  - Species richness
KW  - Coprology
KW  - QPCR
KW  - Anthelmintic resistance
N2  - The faecal egg count reduction test (FECRT) is the most widely used method to assess treatment efficacy against gastrointestinal nematodes (GIN). Information on genera composition of the GIN community is not available with this test and it is commonly obtained by identifying cultured third-stage larvae (L3) or through molecular assays in the post-treatment survey, but results provided are usually only qualitative or semi-quantitative. The updated WAAVP guidelines now recommend assessing anthelmintic efficacy for each GIN genus/species separately (genus-specific FECRT), but this approach is poorly employed in Europe and in goats especially. For this reason, four FECRT trials were conducted using oxfendazole and eprinomectin in two Italian goat farms. Samples were processed individually using the McMaster technique and then pooled to create two samples from faeces of 5 animals each. Pooled samples were analysed using the McMaster and cultured for seven days at 26(degrees)C to obtain L3s. The genus-specific FECRT was based on larval identification, integrating coproculture and FEC results. Larvae were identified as Haemonchus, Trichostrongylus, Teladorsagia, Oesophagostomum / Chabertia and Bunostomum. Molecular assays (a multiplex real-time PCR and two end-point PCRs) were also implemented on pooled samples to support the morphological identification. The Spearmann Rho test confirmed a high correlation between the two approaches (Rho = 0.941 and Rho = 0.914 respectively for Haemonchus and Trichostrongylus, the two most common genera). Both oxfendazole and eprinomectin were effective in one farm, while none in the other farm (FECR = 75.9% and 73.3% respectively). In the second farm, the genus-specific FECRT highlighted a different response to treatment among genera: oxfendazole lacked efficacy against both Haemonchus and Trichostrongylus spp., eprinomectin only against Haemonchus, while all other genera were susceptible to both drugs. This study brings new attention on the importance of adopting a genus-specific approach to identify and quantify differences in susceptibility to anthelmintics among genera in goats, providing support for FECRT interpretation, anthelmintic resistance evaluation and evidence-based GIN control.
ER  -

Základní údaje
Originální název	Faecal egg count reduction test in goats: Zooming in on the genus level
Autoři	MAURIZIO, Anna, Lucie ŠKORPÍKOVÁ, Jana ILGOVÁ, Cinzia TESSARIN, Giorgia DOTTO, Nikol RESLOVÁ, Jaroslav VADLEJCH, Erica MARCHIORI, di Regalbono Antonio FRANGIPANE, Martin KAŠNÝ a Rudi CASSINI.
Vydání	Veterinary Parasitology, AMSTERDAM, Elsevier Science, 2024, 0304-4017.

Další údaje
Originální jazyk	angličtina
Typ výsledku	Článek v odborném periodiku
Utajení	není předmětem státního či obchodního tajemství
Impakt faktor	Impact factor: 2.600 v roce 2022
Organizační jednotka	Přírodovědecká fakulta
Doi	http://dx.doi.org/10.1016/j.vetpar.2024.110146
UT WoS	001199202600001
Klíčová slova anglicky	Gastrointestinal tract; Caprine nematodes; Species richness; Coprology; QPCR; Anthelmintic resistance
Změnil	Změnila: Mgr. et Mgr. Jana Ilgová, Ph.D., učo 223075. Změněno: 6. 5. 2024 09:40.

Anotace
The faecal egg count reduction test (FECRT) is the most widely used method to assess treatment efficacy against gastrointestinal nematodes (GIN). Information on genera composition of the GIN community is not available with this test and it is commonly obtained by identifying cultured third-stage larvae (L3) or through molecular assays in the post-treatment survey, but results provided are usually only qualitative or semi-quantitative. The updated WAAVP guidelines now recommend assessing anthelmintic efficacy for each GIN genus/species separately (genus-specific FECRT), but this approach is poorly employed in Europe and in goats especially. For this reason, four FECRT trials were conducted using oxfendazole and eprinomectin in two Italian goat farms. Samples were processed individually using the McMaster technique and then pooled to create two samples from faeces of 5 animals each. Pooled samples were analysed using the McMaster and cultured for seven days at 26(degrees)C to obtain L3s. The genus-specific FECRT was based on larval identification, integrating coproculture and FEC results. Larvae were identified as Haemonchus, Trichostrongylus, Teladorsagia, Oesophagostomum / Chabertia and Bunostomum. Molecular assays (a multiplex real-time PCR and two end-point PCRs) were also implemented on pooled samples to support the morphological identification. The Spearmann Rho test confirmed a high correlation between the two approaches (Rho = 0.941 and Rho = 0.914 respectively for Haemonchus and Trichostrongylus, the two most common genera). Both oxfendazole and eprinomectin were effective in one farm, while none in the other farm (FECR = 75.9% and 73.3% respectively). In the second farm, the genus-specific FECRT highlighted a different response to treatment among genera: oxfendazole lacked efficacy against both Haemonchus and Trichostrongylus spp., eprinomectin only against Haemonchus, while all other genera were susceptible to both drugs. This study brings new attention on the importance of adopting a genus-specific approach to identify and quantify differences in susceptibility to anthelmintics among genera in goats, providing support for FECRT interpretation, anthelmintic resistance evaluation and evidence-based GIN control.

Anotace

The faecal egg count reduction test (FECRT) is the most widely used method to assess treatment efficacy against gastrointestinal nematodes (GIN). Information on genera composition of the GIN community is not available with this test and it is commonly obtained by identifying cultured third-stage larvae (L3) or through molecular assays in the post-treatment survey, but results provided are usually only qualitative or semi-quantitative. The updated WAAVP guidelines now recommend assessing anthelmintic efficacy for each GIN genus/species separately (genus-specific FECRT), but this approach is poorly employed in Europe and in goats especially. For this reason, four FECRT trials were conducted using oxfendazole and eprinomectin in two Italian goat farms. Samples were processed individually using the McMaster technique and then pooled to create two samples from faeces of 5 animals each. Pooled samples were analysed using the McMaster and cultured for seven days at 26(degrees)C to obtain L3s. The genus-specific FECRT was based on larval identification, integrating coproculture and FEC results. Larvae were identified as Haemonchus, Trichostrongylus, Teladorsagia, Oesophagostomum / Chabertia and Bunostomum. Molecular assays (a multiplex real-time PCR and two end-point PCRs) were also implemented on pooled samples to support the morphological identification. The Spearmann Rho test confirmed a high correlation between the two approaches (Rho = 0.941 and Rho = 0.914 respectively for Haemonchus and Trichostrongylus, the two most common genera). Both oxfendazole and eprinomectin were effective in one farm, while none in the other farm (FECR = 75.9% and 73.3% respectively). In the second farm, the genus-specific FECRT highlighted a different response to treatment among genera: oxfendazole lacked efficacy against both Haemonchus and Trichostrongylus spp., eprinomectin only against Haemonchus, while all other genera were susceptible to both drugs. This study brings new attention on the importance of adopting a genus-specific approach to identify and quantify differences in susceptibility to anthelmintics among genera in goats, providing support for FECRT interpretation, anthelmintic resistance evaluation and evidence-based GIN control.