Application of Chemical Inhibitors in Live Cell Imaging of
Plant Meiosis Using Light Sheet Fluorescence Microscopy

C 2022

Application of Chemical Inhibitors in Live Cell Imaging of Plant Meiosis Using Light Sheet Fluorescence Microscopy

VALUCHOVÁ, Soňa, Pavlína MIKULKOVÁ, Jana PEČINKOVÁ and Karel ŘÍHA

Basic information

Original name

Application of Chemical Inhibitors in Live Cell Imaging of Plant Meiosis Using Light Sheet Fluorescence Microscopy

Authors

VALUCHOVÁ, Soňa (703 Slovakia, belonging to the institution), Pavlína MIKULKOVÁ (203 Czech Republic, belonging to the institution), Jana PEČINKOVÁ (203 Czech Republic, belonging to the institution) and Karel ŘÍHA (203 Czech Republic, guarantor, belonging to the institution)

Edition

Humana New York, NY, Plant Gametogenesis. Methods in Molecular Biology, vol 2484, p. 93-105, 13 pp. Plant Gametogenesis. Methods in Molecular Biology, vol 2484, 2022

Publisher

Humana Press Inc.

Other information

Language

English

Type of outcome

Kapitola resp. kapitoly v odborné knize

Field of Study

10611 Plant sciences, botany

Confidentiality degree

není předmětem státního či obchodního tajemství

Publication form

printed version "print"

References:

URL

Organization unit

Central European Institute of Technology

ISBN

978-1-0716-2252-0

DOI

http://dx.doi.org/10.1007/978-1-0716-2253-7_8

Keywords in English

Arabidopsis; fluorescences; microscopy; meiosis

Abstract

V originále

Live imaging combined with the application of chemical inhibitors is a powerful research tool that enables researchers to precisely time the inhibition of cellular processes and study the consequences of these perturbations. This approach is usually applied to in vitro cultivated cells that are easily accessible to chemical treatments and microscopic observations. Here we describe a method for live cell imaging of Arabidopsis meiocytes embedded within floral organs combined with the application of a chemical drug at desired timepoints during meiosis. We describe a customized solution for the Zeiss Z.1 light sheet microscope, including sample preparation and data processing, and demonstrate its utility for the analysis of meiotic progression upon spindle inhibition.

Links

EF15_003/0000479, research and development project

Name: Regulace rostlinné meiózy

Citovat

VALUCHOVÁ, Soňa, Pavlína MIKULKOVÁ, Jana PEČINKOVÁ and Karel ŘÍHA. Application of Chemical Inhibitors in Live Cell Imaging of Plant Meiosis Using Light Sheet Fluorescence Microscopy. In Plant Gametogenesis. Methods in Molecular Biology, vol 2484. Humana New York, NY: Humana Press Inc., 2022, p. 93-105. Plant Gametogenesis. Methods in Molecular Biology, vol 2484. ISBN 978-1-0716-2252-0. Available from: https://dx.doi.org/10.1007/978-1-0716-2253-7_8.

@inbook{2400457,
   author = {Valuchová, Soňa and Mikulková, Pavlína and Pečinková, Jana and Říha, Karel},
   address = {Humana New York, NY},
   booktitle = {Plant Gametogenesis. Methods in Molecular Biology, vol 2484},
   doi = {http://dx.doi.org/10.1007/978-1-0716-2253-7_8},
   keywords = {Arabidopsis; fluorescences; microscopy; meiosis},
   howpublished = {tištěná verze "print"},
   language = {eng},
   location = {Humana New York, NY},
   isbn = {978-1-0716-2252-0},
   pages = {93-105},
   publisher = {Humana Press Inc.},
   title = {Application of Chemical Inhibitors in Live Cell Imaging of Plant Meiosis Using Light Sheet Fluorescence Microscopy},
   url = {https://link.springer.com/protocol/10.1007/978-1-0716-2253-7_8},
   year = {2022}
}

TY  - CHAP
ID  - 2400457
AU  - Valuchová, Soňa - Mikulková, Pavlína - Pečinková, Jana - Říha, Karel
PY  - 2022
TI  - Application of Chemical Inhibitors in Live Cell Imaging of Plant Meiosis Using Light Sheet Fluorescence Microscopy
VL  - Plant Gametogenesis. Methods in Molecular Biology, vol 2484
PB  - Humana Press Inc.
CY  - Humana New York, NY
SN  - 9781071622520
KW  - Arabidopsis
KW  - fluorescences
KW  - microscopy
KW  - meiosis
UR  - https://link.springer.com/protocol/10.1007/978-1-0716-2253-7_8
N2  - Live imaging combined with the application of chemical inhibitors is a powerful research tool that enables researchers to precisely time the inhibition of cellular processes and study the consequences of these perturbations. This approach is usually applied to in vitro cultivated cells that are easily accessible to chemical treatments and microscopic observations. Here we describe a method for live cell imaging of Arabidopsis meiocytes embedded within floral organs combined with the application of a chemical drug at desired timepoints during meiosis. We describe a customized solution for the Zeiss Z.1 light sheet microscope, including sample preparation and data processing, and demonstrate its utility for the analysis of meiotic progression upon spindle inhibition.
ER  -

VALUCHOVÁ, Soňa, Pavlína MIKULKOVÁ, Jana PEČINKOVÁ and Karel ŘÍHA. Application of Chemical Inhibitors in Live Cell Imaging of Plant Meiosis Using Light Sheet Fluorescence Microscopy. In \textit{Plant Gametogenesis. Methods in Molecular Biology, vol 2484}. Humana New York, NY: Humana Press Inc., 2022, p.~93-105. Plant Gametogenesis. Methods in Molecular Biology, vol 2484. ISBN~978-1-0716-2252-0. Available from: https://dx.doi.org/10.1007/978-1-0716-2253-7\_{}8.

Detailed Information on Publication Record