Dynamic microvilli sculpt bristles at nanometric scale

J 2024

Dynamic microvilli sculpt bristles at nanometric scale

IKEDA, Kyojiro N, Ilya BELEVICH, Luis ZELAYA-LAINEZ, Lukas OREL, Josef FÜSSL et. al.

Basic information

Original name

Dynamic microvilli sculpt bristles at nanometric scale

Authors

IKEDA, Kyojiro N, Ilya BELEVICH, Luis ZELAYA-LAINEZ, Lukas OREL, Josef FÜSSL, Jaromír GUMULEC (203 Czech Republic, belonging to the institution), Christian HELLMICH, Eija JOKITALO and Florian RAIBLE

Edition

Nature Communications, BERLIN, NATURE PORTFOLIO, 2024, 2041-1723

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30105 Physiology

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 16.600 in 2022

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1038/s41467-024-48044-3

UT WoS

001221986200032

Keywords in English

Actin; Morphogenesis; Nanoscale materials

Abstract

V originále

Organisms generate shapes across size scales. Whereas patterning and morphogenesis of macroscopic tissues has been extensively studied, the principles underlying the formation of micrometric and submicrometric structures remain largely enigmatic. Individual cells of polychaete annelids, so-called chaetoblasts, are associated with the generation of chitinous bristles of highly stereotypic geometry. Here we show that bristle formation requires a chitin-producing enzyme specifically expressed in the chaetoblasts. Chaetoblasts exhibit dynamic cell surfaces with stereotypical patterns of actin-rich microvilli. These microvilli can be matched with internal and external structures of bristles reconstructed from serial block-face electron micrographs. Individual chitin teeth are deposited by microvilli in an extension-disassembly cycle resembling a biological 3D printer. Consistently, pharmacological interference with actin dynamics leads to defects in tooth formation. Our study reveals that both material and shape of bristles are encoded by the same cell, and that microvilli play a role in micro- to submicrometric sculpting of biomaterials.

Links

NU22J-08-00062, research and development project

Name: Mechanická a morfologická high-throughput fenotypizace nádorových buněk během smykového napětí: prediktor migračního a invazivního potenciálu

Investor: Ministry of Health of the CR, Subprogram 2 - junior

Citovat

IKEDA, Kyojiro N, Ilya BELEVICH, Luis ZELAYA-LAINEZ, Lukas OREL, Josef FÜSSL, Jaromír GUMULEC, Christian HELLMICH, Eija JOKITALO and Florian RAIBLE. Dynamic microvilli sculpt bristles at nanometric scale. Nature Communications. BERLIN: NATURE PORTFOLIO, 2024, vol. 15, No 1, p. 1-9. ISSN 2041-1723. Available from: https://dx.doi.org/10.1038/s41467-024-48044-3.

@article{2403037,
   author = {Ikeda, Kyojiro N and Belevich, Ilya and ZelayaandLainez, Luis and Orel, Lukas and Füssl, Josef and Gumulec, Jaromír and Hellmich, Christian and Jokitalo, Eija and Raible, Florian},
   article_location = {BERLIN},
   article_number = {1},
   doi = {http://dx.doi.org/10.1038/s41467-024-48044-3},
   keywords = {Actin; Morphogenesis; Nanoscale materials},
   language = {eng},
   issn = {2041-1723},
   journal = {Nature Communications},
   title = {Dynamic microvilli sculpt bristles at nanometric scale},
   url = {https://www.nature.com/articles/s41467-024-48044-3},
   volume = {15},
   year = {2024}
}

TY  - JOUR
ID  - 2403037
AU  - Ikeda, Kyojiro N - Belevich, Ilya - Zelaya-Lainez, Luis - Orel, Lukas - Füssl, Josef - Gumulec, Jaromír - Hellmich, Christian - Jokitalo, Eija - Raible, Florian
PY  - 2024
TI  - Dynamic microvilli sculpt bristles at nanometric scale
JF  - Nature Communications
VL  - 15
IS  - 1
SP  - 1-9
EP  - 1-9
PB  - NATURE PORTFOLIO
SN  - 20411723
KW  - Actin
KW  - Morphogenesis
KW  - Nanoscale materials
UR  - https://www.nature.com/articles/s41467-024-48044-3
N2  - Organisms generate shapes across size scales. Whereas patterning and morphogenesis of macroscopic tissues has been extensively studied, the principles underlying the formation of micrometric and submicrometric structures remain largely enigmatic. Individual cells of polychaete annelids, so-called chaetoblasts, are associated with the generation of chitinous bristles of highly stereotypic geometry. Here we show that bristle formation requires a chitin-producing enzyme specifically expressed in the chaetoblasts. Chaetoblasts exhibit dynamic cell surfaces with stereotypical patterns of actin-rich microvilli. These microvilli can be matched with internal and external structures of bristles reconstructed from serial block-face electron micrographs. Individual chitin teeth are deposited by microvilli in an extension-disassembly cycle resembling a biological 3D printer. Consistently, pharmacological interference with actin dynamics leads to defects in tooth formation. Our study reveals that both material and shape of bristles are encoded by the same cell, and that microvilli play a role in micro- to submicrometric sculpting of biomaterials.
ER  -

IKEDA, Kyojiro N, Ilya BELEVICH, Luis ZELAYA-LAINEZ, Lukas OREL, Josef FÜSSL, Jaromír GUMULEC, Christian HELLMICH, Eija JOKITALO and Florian RAIBLE. Dynamic microvilli sculpt bristles at nanometric scale. \textit{Nature Communications}. BERLIN: NATURE PORTFOLIO, 2024, vol.~15, No~1, p.~1-9. ISSN~2041-1723. Available from: https://dx.doi.org/10.1038/s41467-024-48044-3.

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