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@inbook{2403779,
author = {Čimborová, Katarína and Kotasová, Hana and Pelková, Vendula and Sedláková, Veronika and Hampl, Aleš},
address = {New York},
booktitle = {3D Cell Culture. Methods in Molecular Biology},
doi = {http://dx.doi.org/10.1007/978-1-0716-3674-9_3},
edition = {2.},
editor = {Sumbalova Koledova, Z.},
keywords = {Decellularized scaffolds; peptide hydrogels; Micropatterning; phenotypic evaluation; bone tissue modeling; Biofabrication; Bioprinting; Organoids},
howpublished = {elektronická verze "online"},
language = {eng},
location = {New York},
isbn = {978-1-0716-3673-2},
pages = {21–33},
publisher = {Humana},
title = {Decellularization of Pig Lung to Yield Three-Dimensional Scaffold for Lung Tissue Engineering},
url = {https://link.springer.com/protocol/10.1007/978-1-0716-3674-9_3},
year = {2024}
}
TY - CHAP
ID - 2403779
AU - Čimborová, Katarína - Kotasová, Hana - Pelková, Vendula - Sedláková, Veronika - Hampl, Aleš
PY - 2024
TI - Decellularization of Pig Lung to Yield Three-Dimensional Scaffold for Lung Tissue Engineering
VL - vol 2764
PB - Humana
CY - New York
SN - 9781071636732
KW - Decellularized scaffolds
KW - peptide hydrogels
KW - Micropatterning
KW - phenotypic evaluation
KW - bone tissue modeling
KW - Biofabrication
KW - Bioprinting
KW - Organoids
UR - https://link.springer.com/protocol/10.1007/978-1-0716-3674-9_3
N2 - Lately, the need for three-dimensional (3D) cell culture has been recognized in order to closely mimic the organization of native tissues. Thus, 3D scaffolds started to be employed to facilitate the 3D cell organization and enable the artificial tissue formation for the emerging tissue engineering applications. 3D scaffolds can be prepared by various techniques, each with certain advantages and disadvantages. Decellularization is an easy method based on removal of cells from native tissue sample, yielding extracellular matrix (ECM) scaffold with preserved architecture and bioactivity. This chapter provides a detailed protocol for decellularization of pig lung and also some basic assays for evaluation of its effectivity, such as determination of DNA content and histological verification of the selected ECM components. Such decellularized scaffold can subsequently be used for various tissue engineering applications, for example, for recellularization with cells of interest, for natural ECM hydrogel preparation, or as a bioink for 3D bioprinting.
ER -
ČIMBOROVÁ, Katarína, Hana KOTASOVÁ, Vendula PELKOVÁ, Veronika SEDLÁKOVÁ and Aleš HAMPL. Decellularization of Pig Lung to Yield Three-Dimensional Scaffold for Lung Tissue Engineering. Online. In Sumbalova Koledova, Z. \textit{3D Cell Culture. Methods in Molecular Biology}. 2nd ed. New York: Humana, 2024, p.~21–33. vol 2764. ISBN~978-1-0716-3673-2. Available from: https://dx.doi.org/10.1007/978-1-0716-3674-9\_{}3.
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