Decellularization of Pig Lung to Yield Three-Dimensional
Scaffold for Lung Tissue Engineering

C 2024

Decellularization of Pig Lung to Yield Three-Dimensional Scaffold for Lung Tissue Engineering

ČIMBOROVÁ, Katarína, Hana KOTASOVÁ, Vendula PELKOVÁ, Veronika SEDLÁKOVÁ, Aleš HAMPL et. al.

Basic information

Original name

Decellularization of Pig Lung to Yield Three-Dimensional Scaffold for Lung Tissue Engineering

Authors

ČIMBOROVÁ, Katarína (703 Slovakia, belonging to the institution), Hana KOTASOVÁ (203 Czech Republic, belonging to the institution), Vendula PELKOVÁ (203 Czech Republic, belonging to the institution), Veronika SEDLÁKOVÁ (203 Czech Republic, belonging to the institution) and Aleš HAMPL (203 Czech Republic, belonging to the institution)

Edition

2. vyd. New York, 3D Cell Culture. Methods in Molecular Biology, p. 21-33, 13 pp. vol 2764, 2024

Publisher

Humana

Other information

Language

English

Type of outcome

Kapitola resp. kapitoly v odborné knize

Field of Study

10601 Cell biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Publication form

electronic version available online

References:

URL

Organization unit

Faculty of Medicine

ISBN

978-1-0716-3673-2

DOI

http://dx.doi.org/10.1007/978-1-0716-3674-9_3

Keywords in English

Decellularized scaffolds; peptide hydrogels; Micropatterning; phenotypic evaluation; bone tissue modeling; Biofabrication; Bioprinting; Organoids

Abstract

V originále

Lately, the need for three-dimensional (3D) cell culture has been recognized in order to closely mimic the organization of native tissues. Thus, 3D scaffolds started to be employed to facilitate the 3D cell organization and enable the artificial tissue formation for the emerging tissue engineering applications. 3D scaffolds can be prepared by various techniques, each with certain advantages and disadvantages. Decellularization is an easy method based on removal of cells from native tissue sample, yielding extracellular matrix (ECM) scaffold with preserved architecture and bioactivity. This chapter provides a detailed protocol for decellularization of pig lung and also some basic assays for evaluation of its effectivity, such as determination of DNA content and histological verification of the selected ECM components. Such decellularized scaffold can subsequently be used for various tissue engineering applications, for example, for recellularization with cells of interest, for natural ECM hydrogel preparation, or as a bioink for 3D bioprinting.

Links

MUNI/A/1398/2021, interní kód MU

Name: Zdroje pro tkáňové inženýrství 12 (Acronym: TissueEng 12)

Investor: Masaryk University

NV16-31501A, research and development project

Name: Tkáňové inženýrství epitelů: Buňky a protokoly pro regenerativní medicínu

Citovat

ČIMBOROVÁ, Katarína, Hana KOTASOVÁ, Vendula PELKOVÁ, Veronika SEDLÁKOVÁ and Aleš HAMPL. Decellularization of Pig Lung to Yield Three-Dimensional Scaffold for Lung Tissue Engineering. Online. In Sumbalova Koledova, Z. 3D Cell Culture. Methods in Molecular Biology. 2nd ed. New York: Humana, 2024, p. 21-33. vol 2764. ISBN 978-1-0716-3673-2. Available from: https://dx.doi.org/10.1007/978-1-0716-3674-9_3.

@inbook{2403779,
   author = {Čimborová, Katarína and Kotasová, Hana and Pelková, Vendula and Sedláková, Veronika and Hampl, Aleš},
   address = {New York},
   booktitle = {3D Cell Culture. Methods in Molecular Biology},
   doi = {http://dx.doi.org/10.1007/978-1-0716-3674-9_3},
   edition = {2.},
   editor = {Sumbalova Koledova, Z.},
   keywords = {Decellularized scaffolds; peptide hydrogels; Micropatterning; phenotypic evaluation; bone tissue modeling; Biofabrication; Bioprinting; Organoids},
   howpublished = {elektronická verze "online"},
   language = {eng},
   location = {New York},
   isbn = {978-1-0716-3673-2},
   pages = {21-33},
   publisher = {Humana},
   title = {Decellularization of Pig Lung to Yield Three-Dimensional Scaffold for Lung Tissue Engineering},
   url = {https://link.springer.com/protocol/10.1007/978-1-0716-3674-9_3},
   year = {2024}
}

TY  - CHAP
ID  - 2403779
AU  - Čimborová, Katarína - Kotasová, Hana - Pelková, Vendula - Sedláková, Veronika - Hampl, Aleš
PY  - 2024
TI  - Decellularization of Pig Lung to Yield Three-Dimensional Scaffold for Lung Tissue Engineering
VL  - vol 2764
PB  - Humana
CY  - New York
SN  - 9781071636732
KW  - Decellularized scaffolds
KW  - peptide hydrogels
KW  - Micropatterning
KW  - phenotypic evaluation
KW  - bone tissue modeling
KW  - Biofabrication
KW  - Bioprinting
KW  - Organoids
UR  - https://link.springer.com/protocol/10.1007/978-1-0716-3674-9_3
N2  - Lately, the need for three-dimensional (3D) cell culture has been recognized in order to closely mimic the organization of native tissues. Thus, 3D scaffolds started to be employed to facilitate the 3D cell organization and enable the artificial tissue formation for the emerging tissue engineering applications. 3D scaffolds can be prepared by various techniques, each with certain advantages and disadvantages. Decellularization is an easy method based on removal of cells from native tissue sample, yielding extracellular matrix (ECM) scaffold with preserved architecture and bioactivity. This chapter provides a detailed protocol for decellularization of pig lung and also some basic assays for evaluation of its effectivity, such as determination of DNA content and histological verification of the selected ECM components. Such decellularized scaffold can subsequently be used for various tissue engineering applications, for example, for recellularization with cells of interest, for natural ECM hydrogel preparation, or as a bioink for 3D bioprinting.
ER  -

ČIMBOROVÁ, Katarína, Hana KOTASOVÁ, Vendula PELKOVÁ, Veronika SEDLÁKOVÁ and Aleš HAMPL. Decellularization of Pig Lung to Yield Three-Dimensional Scaffold for Lung Tissue Engineering. Online. In Sumbalova Koledova, Z. \textit{3D Cell Culture. Methods in Molecular Biology}. 2nd ed. New York: Humana, 2024, p.~21-33. vol 2764. ISBN~978-1-0716-3673-2. Available from: https://dx.doi.org/10.1007/978-1-0716-3674-9\_{}3.

Detailed Information on Publication Record