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@inbook{2403779, author = {Čimborová, Katarína and Kotasová, Hana and Pelková, Vendula and Sedláková, Veronika and Hampl, Aleš}, address = {New York}, booktitle = {3D Cell Culture. Methods in Molecular Biology}, doi = {http://dx.doi.org/10.1007/978-1-0716-3674-9_3}, edition = {2.}, editor = {Sumbalova Koledova, Z.}, keywords = {Decellularized scaffolds; peptide hydrogels; Micropatterning; phenotypic evaluation; bone tissue modeling; Biofabrication; Bioprinting; Organoids}, howpublished = {elektronická verze "online"}, language = {eng}, location = {New York}, isbn = {978-1-0716-3673-2}, pages = {21-33}, publisher = {Humana}, title = {Decellularization of Pig Lung to Yield Three-Dimensional Scaffold for Lung Tissue Engineering}, url = {https://link.springer.com/protocol/10.1007/978-1-0716-3674-9_3}, year = {2024} }
TY - CHAP ID - 2403779 AU - Čimborová, Katarína - Kotasová, Hana - Pelková, Vendula - Sedláková, Veronika - Hampl, Aleš PY - 2024 TI - Decellularization of Pig Lung to Yield Three-Dimensional Scaffold for Lung Tissue Engineering VL - vol 2764 PB - Humana CY - New York SN - 9781071636732 KW - Decellularized scaffolds KW - peptide hydrogels KW - Micropatterning KW - phenotypic evaluation KW - bone tissue modeling KW - Biofabrication KW - Bioprinting KW - Organoids UR - https://link.springer.com/protocol/10.1007/978-1-0716-3674-9_3 N2 - Lately, the need for three-dimensional (3D) cell culture has been recognized in order to closely mimic the organization of native tissues. Thus, 3D scaffolds started to be employed to facilitate the 3D cell organization and enable the artificial tissue formation for the emerging tissue engineering applications. 3D scaffolds can be prepared by various techniques, each with certain advantages and disadvantages. Decellularization is an easy method based on removal of cells from native tissue sample, yielding extracellular matrix (ECM) scaffold with preserved architecture and bioactivity. This chapter provides a detailed protocol for decellularization of pig lung and also some basic assays for evaluation of its effectivity, such as determination of DNA content and histological verification of the selected ECM components. Such decellularized scaffold can subsequently be used for various tissue engineering applications, for example, for recellularization with cells of interest, for natural ECM hydrogel preparation, or as a bioink for 3D bioprinting. ER -
ČIMBOROVÁ, Katarína, Hana KOTASOVÁ, Vendula PELKOVÁ, Veronika SEDLÁKOVÁ and Aleš HAMPL. Decellularization of Pig Lung to Yield Three-Dimensional Scaffold for Lung Tissue Engineering. Online. In Sumbalova Koledova, Z. \textit{3D Cell Culture. Methods in Molecular Biology}. 2nd ed. New York: Humana, 2024, p.~21-33. vol 2764. ISBN~978-1-0716-3673-2. Available from: https://dx.doi.org/10.1007/978-1-0716-3674-9\_{}3.
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