Truncated vitronectin with e-cadherin enables the xeno-free derivation ofhuman embryonic stem cells

SOURALOVÁ, Tereza, Daniela HULÍNOVÁ, Pavel VENTRUBA, Michal JEŠETA, Aleš HAMPL and Irena KOUTNÁ. Truncated vitronectin with e-cadherin enables the xeno-free derivation ofhuman embryonic stem cells. In 23rd Interdisciplinary Meeting of Young Biologists, Biochemists, and Chemists, Milovy. 2024.

Basic information

• Original name: Truncated vitronectin with e-cadherin enables the xeno-free derivation ofhuman embryonic stem cells
• Authors: SOURALOVÁ, Tereza, Daniela HULÍNOVÁ, Pavel VENTRUBA, Michal JEŠETA, Aleš HAMPL and Irena KOUTNÁ.
• Edition: 23rd Interdisciplinary Meeting of Young Biologists, Biochemists, and Chemists, Milovy, 2024.

Other information

• Original language: English
• Type of outcome: Presentations at conferences
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• WWW: ISSN 2336-7210
• Tags: Reviewed

Abstract

Human embryonic stem cells (hESCs) are able to differentiate into any cell type of a human body, have self-renewal abilities and very high proliferative activity1. These unique abilities enable their use in cell therapy, disease modelling, and drug development. The derivation of hESCs is usually performed using an animal or human cell feeder layer, which is undefined and potentially contagious, and because of these difficulties, there is a tendency to replace feeders with xeno-free defined substrates in recent years2.We used truncated vitronectin with E-cadherin as adefined xeno-free substrate for the derivation of hESCs for the first time, derived three hESC lines, and confirmed their undifferentiated state, hESC morphology, and standard karyotypes together with their potential to differentiate into three germ layers (ectoderm, mesoderm, and endoderm)3. We confirmed for the first time that truncated vitronectin with E-cadherin is a defined xeno-free substrate that is suitable for the derivation of hESCs involved in cell therapies.

Links

• MUNI/A/1301/2022, interní kód MU: Name: Zdroje pro tkáňové inženýrství 13

Investor: Masaryk University

• MUNI/A/1598/2023, interní kód MU: Name: Zdroje pro tkáňové inženýrství 14

Investor: Masaryk University, Resources pro tissue engineering 14

• MUNI/IGA/1332/2021, interní kód MU: Name: The impact of derivation substrate on differentiation of human embryonic stem cells for the cell therapy

Investor: Masaryk University

• NU22-08-00629, research and development project: Name: Vývoj postupů pro léčbu makulární degenerace sítnice deriváty lidských pluripotentních kmenových buněk

Investor: Ministry of Health of the CR, Subprogram 1 - standard