ŠTEFANOVIE, Barbora, Leon Paul JENNER, Lucie BOZDĚCHOVÁ, Petr FAJKUS, Eva SÝKOROVÁ, Jiří FAJKUS and Jan PALEČEK. Characterisation of the Arabidopsis thaliana telomerase TERT-TR complex. Plant Molecular Biology. Dordrecht (Netherlands): Springer, 2024, vol. 114, No 3, p. 1-12. ISSN 0167-4412. Available from: https://dx.doi.org/10.1007/s11103-024-01461-w. |
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@article{2405278, author = {Štefanovie, Barbora and Jenner, Leon Paul and Bozděchová, Lucie and Fajkus, Petr and Sýkorová, Eva and Fajkus, Jiří and Paleček, Jan}, article_location = {Dordrecht (Netherlands)}, article_number = {3}, doi = {http://dx.doi.org/10.1007/s11103-024-01461-w}, keywords = {A.thaliana telomerase; AtTERT; AtTR; Protein-RNA interactions; Yeast three-hybrid}, language = {eng}, issn = {0167-4412}, journal = {Plant Molecular Biology}, title = {Characterisation of the Arabidopsis thaliana telomerase TERT-TR complex}, url = {https://link.springer.com/article/10.1007/s11103-024-01461-w}, volume = {114}, year = {2024} }
TY - JOUR ID - 2405278 AU - Štefanovie, Barbora - Jenner, Leon Paul - Bozděchová, Lucie - Fajkus, Petr - Sýkorová, Eva - Fajkus, Jiří - Paleček, Jan PY - 2024 TI - Characterisation of the Arabidopsis thaliana telomerase TERT-TR complex JF - Plant Molecular Biology VL - 114 IS - 3 SP - 1-12 EP - 1-12 PB - Springer SN - 01674412 KW - A.thaliana telomerase KW - AtTERT KW - AtTR KW - Protein-RNA interactions KW - Yeast three-hybrid UR - https://link.springer.com/article/10.1007/s11103-024-01461-w N2 - Most eukaryotic organisms employ a telomerase complex for the maintenance of chromosome ends. The core of this complex is composed of telomerase reverse transcriptase (TERT) and telomerase RNA (TR) subunits. The TERT reverse transcriptase (RT) domain synthesises telomeric DNA using the TR template sequence. The other TERT domains contribute to this process in different ways. In particular, the TERT RNA-binding domain (TRBD) interacts with specific TR motif(s). Using a yeast 3-hybrid system, we show the critical role of Arabidopsis thaliana (At) TRBD and embryophyta-conserved KRxR motif in the unstructured linker preceding the TRBD domain for binding to the recently identified AtTR subunit. We also show the essential role of the predicted P4 stem and pseudoknot AtTR structures and provide evidence for the binding of AtTRBD to pseudoknot and KRxR motif stabilising interaction with the P4 stem structure. Our results thus provide the first insight into the core part of the plant telomerase complex. ER -
ŠTEFANOVIE, Barbora, Leon Paul JENNER, Lucie BOZDĚCHOVÁ, Petr FAJKUS, Eva SÝKOROVÁ, Jiří FAJKUS and Jan PALEČEK. Characterisation of the Arabidopsis thaliana telomerase TERT-TR complex. \textit{Plant Molecular Biology}. Dordrecht (Netherlands): Springer, 2024, vol.~114, No~3, p.~1-12. ISSN~0167-4412. Available from: https://dx.doi.org/10.1007/s11103-024-01461-w.
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