Characterisation of the Arabidopsis thaliana telomerase TERT-TR
complex

J 2024

Characterisation of the Arabidopsis thaliana telomerase TERT-TR complex

ŠTEFANOVIE, Barbora, Leon Paul JENNER, Lucie BOZDĚCHOVÁ, Petr FAJKUS, Eva SÝKOROVÁ et. al.

Basic information

Original name

Characterisation of the Arabidopsis thaliana telomerase TERT-TR complex

Authors

ŠTEFANOVIE, Barbora (703 Slovakia, belonging to the institution), Leon Paul JENNER (826 United Kingdom of Great Britain and Northern Ireland), Lucie BOZDĚCHOVÁ (203 Czech Republic, belonging to the institution), Petr FAJKUS (203 Czech Republic, belonging to the institution), Eva SÝKOROVÁ (203 Czech Republic), Jiří FAJKUS (203 Czech Republic, belonging to the institution) and Jan PALEČEK (203 Czech Republic, guarantor, belonging to the institution)

Edition

Plant Molecular Biology, Dordrecht (Netherlands), Springer, 2024, 0167-4412

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.100 in 2022

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1007/s11103-024-01461-w

UT WoS

001222709400002

Keywords (in Czech)

A.thaliana telomerasa; AtTERT; AtTR; Protein-RNA interakce; kvasinkový troj-hybridní systém

Keywords in English

A.thaliana telomerase; AtTERT; AtTR; Protein-RNA interactions; Yeast three-hybrid

Abstract

V originále

Most eukaryotic organisms employ a telomerase complex for the maintenance of chromosome ends. The core of this complex is composed of telomerase reverse transcriptase (TERT) and telomerase RNA (TR) subunits. The TERT reverse transcriptase (RT) domain synthesises telomeric DNA using the TR template sequence. The other TERT domains contribute to this process in different ways. In particular, the TERT RNA-binding domain (TRBD) interacts with specific TR motif(s). Using a yeast 3-hybrid system, we show the critical role of Arabidopsis thaliana (At) TRBD and embryophyta-conserved KRxR motif in the unstructured linker preceding the TRBD domain for binding to the recently identified AtTR subunit. We also show the essential role of the predicted P4 stem and pseudoknot AtTR structures and provide evidence for the binding of AtTRBD to pseudoknot and KRxR motif stabilising interaction with the P4 stem structure. Our results thus provide the first insight into the core part of the plant telomerase complex.

Links

EH22_008/0004581, research and development project

Name: Nové poznatky pro plodiny nové generace

GX20-01331X, research and development project

Name: Biogeneze a evoluce telomerázy u rostlin

Investor: Czech Science Foundation

MUNI/R/1142/2021, interní kód MU

Name: Visualization of the 3D structure of chromatin

Investor: Masaryk University, CAREER RESTART

Files attached

PMB-Stefanovie24_Characterisation_of_the_Arabidopsis_thaliana_telomerase_TERT-TR_complex.pdf

Find similar documents

Citovat

ŠTEFANOVIE, Barbora, Leon Paul JENNER, Lucie BOZDĚCHOVÁ, Petr FAJKUS, Eva SÝKOROVÁ, Jiří FAJKUS and Jan PALEČEK. Characterisation of the Arabidopsis thaliana telomerase TERT-TR complex. Plant Molecular Biology. Dordrecht (Netherlands): Springer, 2024, vol. 114, No 3, p. 1-12. ISSN 0167-4412. Available from: https://dx.doi.org/10.1007/s11103-024-01461-w.

@article{2405278,
   author = {Štefanovie, Barbora and Jenner, Leon Paul and Bozděchová, Lucie and Fajkus, Petr and Sýkorová, Eva and Fajkus, Jiří and Paleček, Jan},
   article_location = {Dordrecht (Netherlands)},
   article_number = {3},
   doi = {http://dx.doi.org/10.1007/s11103-024-01461-w},
   keywords = {A.thaliana telomerase; AtTERT; AtTR; Protein-RNA interactions; Yeast three-hybrid},
   language = {eng},
   issn = {0167-4412},
   journal = {Plant Molecular Biology},
   title = {Characterisation of the Arabidopsis thaliana telomerase TERT-TR complex},
   url = {https://link.springer.com/article/10.1007/s11103-024-01461-w},
   volume = {114},
   year = {2024}
}

TY  - JOUR
ID  - 2405278
AU  - Štefanovie, Barbora - Jenner, Leon Paul - Bozděchová, Lucie - Fajkus, Petr - Sýkorová, Eva - Fajkus, Jiří - Paleček, Jan
PY  - 2024
TI  - Characterisation of the Arabidopsis thaliana telomerase TERT-TR complex
JF  - Plant Molecular Biology
VL  - 114
IS  - 3
SP  - 1-12
EP  - 1-12
PB  - Springer
SN  - 01674412
KW  - A.thaliana telomerase
KW  - AtTERT
KW  - AtTR
KW  - Protein-RNA interactions
KW  - Yeast three-hybrid
UR  - https://link.springer.com/article/10.1007/s11103-024-01461-w
N2  - Most eukaryotic organisms employ a telomerase complex for the maintenance of chromosome ends. The core of this complex is composed of telomerase reverse transcriptase (TERT) and telomerase RNA (TR) subunits. The TERT reverse transcriptase (RT) domain synthesises telomeric DNA using the TR template sequence. The other TERT domains contribute to this process in different ways. In particular, the TERT RNA-binding domain (TRBD) interacts with specific TR motif(s). Using a yeast 3-hybrid system, we show the critical role of Arabidopsis thaliana (At) TRBD and embryophyta-conserved KRxR motif in the unstructured linker preceding the TRBD domain for binding to the recently identified AtTR subunit. We also show the essential role of the predicted P4 stem and pseudoknot AtTR structures and provide evidence for the binding of AtTRBD to pseudoknot and KRxR motif stabilising interaction with the P4 stem structure. Our results thus provide the first insight into the core part of the plant telomerase complex.
ER  -

ŠTEFANOVIE, Barbora, Leon Paul JENNER, Lucie BOZDĚCHOVÁ, Petr FAJKUS, Eva SÝKOROVÁ, Jiří FAJKUS and Jan PALEČEK. Characterisation of the Arabidopsis thaliana telomerase TERT-TR complex. \textit{Plant Molecular Biology}. Dordrecht (Netherlands): Springer, 2024, vol.~114, No~3, p.~1-12. ISSN~0167-4412. Available from: https://dx.doi.org/10.1007/s11103-024-01461-w.

Detailed Information on Publication Record