Concurrent D-loop cleavage by Mus81 and Yen1 yields half-crossover precursors

CARREIRA, Raquel, Tomas LAMA-DIAZ, Maria CRUGEIRAS, F Javier AGUADO, Marek ŠEBESTA, Lumír KREJČÍ and Miguel G BLANCO. Concurrent D-loop cleavage by Mus81 and Yen1 yields half-crossover precursors. Nucleic Acids Research. OXFORD: OXFORD UNIV PRESS, 2024. ISSN 0305-1048. Available from: https://dx.doi.org/10.1093/nar/gkae453.

Basic information

• Original name: Concurrent D-loop cleavage by Mus81 and Yen1 yields half-crossover precursors
• Authors: CARREIRA, Raquel, Tomas LAMA-DIAZ, Maria CRUGEIRAS, F Javier AGUADO, Marek ŠEBESTA, Lumír KREJČÍ and Miguel G BLANCO.
• Edition: Nucleic Acids Research, OXFORD, OXFORD UNIV PRESS, 2024, 0305-1048.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Country of publisher: United Kingdom of Great Britain and Northern Ireland
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 14.900 in 2022
• Organization unit: Faculty of Medicine
• Doi: http://dx.doi.org/10.1093/nar/gkae453
• UT WoS: 001237859900001
• Tags: 14110513
• Tags: International impact, Reviewed

Abstract

Homologous recombination involves the formation of branched DNA molecules that may interfere with chromosome segregation. To resolve these persistent joint molecules, cells rely on the activation of structure-selective endonucleases (SSEs) during the late stages of the cell cycle. However, the premature activation of SSEs compromises genome integrity, due to untimely processing of replication and/or recombination intermediates. Here, we used a biochemical approach to show that the budding yeast SSEs Mus81 and Yen1 possess the ability to cleave the central recombination intermediate known as the displacement loop or D-loop. Moreover, we demonstrate that, consistently with previous genetic data, the simultaneous action of Mus81 and Yen1, followed by ligation, is sufficient to recreate the formation of a half-crossover precursor in vitro. Our results provide not only mechanistic explanation for the formation of a half-crossover, but also highlight the critical importance for precise regulation of these SSEs to prevent chromosomal rearrangements.

Links

• GX21-22593X, research and development project: Name: Identifikace a charakterizace proteinů zahrnutých v metabolismu G-kvadruplexů a R-smyček a jejich vztah k replikaci DNA
• 206292/E/17/Z, interní kód MU: Name: Mechanics and execution of homologous recombination - biophysics to the organism

Investor: Wellcome Trust