Concurrent D-loop cleavage by Mus81 and Yen1 yields
half-crossover precursors

J 2024

Concurrent D-loop cleavage by Mus81 and Yen1 yields half-crossover precursors

CARREIRA, Raquel, Tomas LAMA-DIAZ, Maria CRUGEIRAS, F Javier AGUADO, Marek ŠEBESTA et. al.

Basic information

Original name

Concurrent D-loop cleavage by Mus81 and Yen1 yields half-crossover precursors

Authors

CARREIRA, Raquel, Tomas LAMA-DIAZ, Maria CRUGEIRAS, F Javier AGUADO, Marek ŠEBESTA, Lumír KREJČÍ and Miguel G BLANCO

Edition

Nucleic Acids Research, OXFORD, OXFORD UNIV PRESS, 2024, 0305-1048

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 14.900 in 2022

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1093/nar/gkae453

UT WoS

001237859900001

Abstract

V originále

Homologous recombination involves the formation of branched DNA molecules that may interfere with chromosome segregation. To resolve these persistent joint molecules, cells rely on the activation of structure-selective endonucleases (SSEs) during the late stages of the cell cycle. However, the premature activation of SSEs compromises genome integrity, due to untimely processing of replication and/or recombination intermediates. Here, we used a biochemical approach to show that the budding yeast SSEs Mus81 and Yen1 possess the ability to cleave the central recombination intermediate known as the displacement loop or D-loop. Moreover, we demonstrate that, consistently with previous genetic data, the simultaneous action of Mus81 and Yen1, followed by ligation, is sufficient to recreate the formation of a half-crossover precursor in vitro. Our results provide not only mechanistic explanation for the formation of a half-crossover, but also highlight the critical importance for precise regulation of these SSEs to prevent chromosomal rearrangements.

Links

GX21-22593X, research and development project

Name: Identifikace a charakterizace proteinů zahrnutých v metabolismu G-kvadruplexů a R-smyček a jejich vztah k replikaci DNA

206292/E/17/Z, interní kód MU

Name: Mechanics and execution of homologous recombination - biophysics to the organism

Investor: Wellcome Trust

Citovat

CARREIRA, Raquel, Tomas LAMA-DIAZ, Maria CRUGEIRAS, F Javier AGUADO, Marek ŠEBESTA, Lumír KREJČÍ and Miguel G BLANCO. Concurrent D-loop cleavage by Mus81 and Yen1 yields half-crossover precursors. Nucleic Acids Research. OXFORD: OXFORD UNIV PRESS, 2024. ISSN 0305-1048. Available from: https://dx.doi.org/10.1093/nar/gkae453.

@article{2407841,
   author = {Carreira, Raquel and LamaandDiaz, Tomas and Crugeiras, Maria and Aguado, F Javier and Šebesta, Marek and Krejčí, Lumír and Blanco, Miguel G},
   article_location = {OXFORD},
   doi = {http://dx.doi.org/10.1093/nar/gkae453},
   language = {eng},
   issn = {0305-1048},
   journal = {Nucleic Acids Research},
   title = {Concurrent D-loop cleavage by Mus81 and Yen1 yields half-crossover precursors},
   url = {https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkae453/7687436?login=true},
   year = {2024}
}

TY  - JOUR
ID  - 2407841
AU  - Carreira, Raquel - Lama-Diaz, Tomas - Crugeiras, Maria - Aguado, F Javier - Šebesta, Marek - Krejčí, Lumír - Blanco, Miguel G
PY  - 2024
TI  - Concurrent D-loop cleavage by Mus81 and Yen1 yields half-crossover precursors
JF  - Nucleic Acids Research
PB  - OXFORD UNIV PRESS
SN  - 03051048
UR  - https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkae453/7687436?login=true
N2  - Homologous recombination involves the formation of branched DNA molecules that may interfere with chromosome segregation. To resolve these persistent joint molecules, cells rely on the activation of structure-selective endonucleases (SSEs) during the late stages of the cell cycle. However, the premature activation of SSEs compromises genome integrity, due to untimely processing of replication and/or recombination intermediates. Here, we used a biochemical approach to show that the budding yeast SSEs Mus81 and Yen1 possess the ability to cleave the central recombination intermediate known as the displacement loop or D-loop. Moreover, we demonstrate that, consistently with previous genetic data, the simultaneous action of Mus81 and Yen1, followed by ligation, is sufficient to recreate the formation of a half-crossover precursor in vitro. Our results provide not only mechanistic explanation for the formation of a half-crossover, but also highlight the critical importance for precise regulation of these SSEs to prevent chromosomal rearrangements.
ER  -

CARREIRA, Raquel, Tomas LAMA-DIAZ, Maria CRUGEIRAS, F Javier AGUADO, Marek ŠEBESTA, Lumír KREJČÍ and Miguel G BLANCO. Concurrent D-loop cleavage by Mus81 and Yen1 yields half-crossover precursors. \textit{Nucleic Acids Research}. OXFORD: OXFORD UNIV PRESS, 2024. ISSN~0305-1048. Available from: https://dx.doi.org/10.1093/nar/gkae453.

Detailed Information on Publication Record