BOROWIEC, Blanka Maria, Wieslawa KRANC, Magdalena KULUS, Patrycja SUJKA-KORDOWSKA, Aneta KONWERSKA, Piotr CELICHOWSKI, Malgorzata JOZKOWIAK, Jakub KULUS, Malgorzata BLATKIEWICZ, Michal JEŠETA, Paul MOZDZIAK and Bartosz KEMPISTY. New Gene Markers Regulating the Process of Vascularization in Pig Preovulatory Oocytes during Growth and Maturation. Journal of biological regulators and homeostatic agents. Silva Marina: Biolife SAS, 2024, vol. 38, No 4, p. 2807-2824. ISSN 0393-974X. Available from: https://dx.doi.org/10.23812/j.biol.regul.homeost.agents.20243804.219. |
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@article{2407938, author = {Borowiec, Blanka Maria and Kranc, Wieslawa and Kulus, Magdalena and SujkaandKordowska, Patrycja and Konwerska, Aneta and Celichowski, Piotr and Jozkowiak, Malgorzata and Kulus, Jakub and Blatkiewicz, Malgorzata and Ješeta, Michal and Mozdziak, Paul and Kempisty, Bartosz}, article_location = {Silva Marina}, article_number = {4}, doi = {http://dx.doi.org/10.23812/j.biol.regul.homeost.agents.20243804.219}, keywords = {cumulus-oocyte complexes; cumulus cells; gene ontology; ovary; sus scrofa; VEGFA}, language = {eng}, issn = {0393-974X}, journal = {Journal of biological regulators and homeostatic agents}, title = {New Gene Markers Regulating the Process of Vascularization in Pig Preovulatory Oocytes during Growth and Maturation}, url = {https://www.biolifesas.org/EN/10.23812/j.biol.regul.homeost.agents.20243804.219}, volume = {38}, year = {2024} }
TY - JOUR ID - 2407938 AU - Borowiec, Blanka Maria - Kranc, Wieslawa - Kulus, Magdalena - Sujka-Kordowska, Patrycja - Konwerska, Aneta - Celichowski, Piotr - Jozkowiak, Malgorzata - Kulus, Jakub - Blatkiewicz, Malgorzata - Ješeta, Michal - Mozdziak, Paul - Kempisty, Bartosz PY - 2024 TI - New Gene Markers Regulating the Process of Vascularization in Pig Preovulatory Oocytes during Growth and Maturation JF - Journal of biological regulators and homeostatic agents VL - 38 IS - 4 SP - 2807-2824 EP - 2807-2824 PB - Biolife SAS SN - 0393974X KW - cumulus-oocyte complexes KW - cumulus cells KW - gene ontology KW - ovary KW - sus scrofa KW - VEGFA UR - https://www.biolifesas.org/EN/10.23812/j.biol.regul.homeost.agents.20243804.219 N2 - Background: A frequent reason for in vitro fertilization failure is insufficient oocyte competence and difficulties in mimicking optimal in vivo conditions at the stage of in vitro maturation. This study aimed to identify the expression profile of genes associated with "vasculature development", "circulatory system process", "hemopoiesis", "patterning of blood vessels" and "hemopoietic or lymphoid organ development" ontological groups during in vitro maturation of domestic pig oocytes. The goal was also to describe new genetic markers that can be used in assisted reproduction techniques. Methods: Porcine oocytes were subjected to in vitro maturation, and the expression of selected genes was compared before and after maturation. Microarray and reverse transcription quantitative polymerase chain reaction (RT-qPCR) analysis were performed on total RNA isolated from the samples. Additionally, immunocytochemical staining was performed and the histological morphology of ovaries was examined. Results: Regardless of the individual processes, the gene expression pattern remained the same and all 32 gene ontology biological process term-genes were represented in down-regulated gene sets. The greatest change in expression was shown by the Vascular Endothelial Growth Factor A (VEGFA) and the smallest by myoferlin (MYOF). Moreover, the expression of VEGFA was confirmed by immunohistochemical analysis of ovaries. Conclusions: The potential contribution of chosen genes to oocyte performance during in vitro maturation can enhance comprehension of the underlying processes in oocytes and their microenvironment. It may also improve the effectiveness of in vitro fertilization. ER -
BOROWIEC, Blanka Maria, Wieslawa KRANC, Magdalena KULUS, Patrycja SUJKA-KORDOWSKA, Aneta KONWERSKA, Piotr CELICHOWSKI, Malgorzata JOZKOWIAK, Jakub KULUS, Malgorzata BLATKIEWICZ, Michal JEŠETA, Paul MOZDZIAK and Bartosz KEMPISTY. New Gene Markers Regulating the Process of Vascularization in Pig Preovulatory Oocytes during Growth and Maturation. \textit{Journal of biological regulators and homeostatic agents}. Silva Marina: Biolife SAS, 2024, vol.~38, No~4, p.~2807-2824. ISSN~0393-974X. Available from: https://dx.doi.org/10.23812/j.biol.regul.homeost.agents.20243804.219.
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