New Gene Markers Regulating the Process of Vascularization in
Pig Preovulatory Oocytes during Growth and Maturation

J 2024

New Gene Markers Regulating the Process of Vascularization in Pig Preovulatory Oocytes during Growth and Maturation

BOROWIEC, Blanka Maria, Wieslawa KRANC, Magdalena KULUS, Patrycja SUJKA-KORDOWSKA, Aneta KONWERSKA et. al.

Basic information

Original name

New Gene Markers Regulating the Process of Vascularization in Pig Preovulatory Oocytes during Growth and Maturation

Authors

Edition

Journal of biological regulators and homeostatic agents, Silva Marina, Biolife SAS, 2024, 0393-974X

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30214 Obstetrics and gynaecology

Country of publisher

Italy

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.200 in 2022

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.23812/j.biol.regul.homeost.agents.20243804.219

UT WoS

001203519300001

Keywords in English

cumulus-oocyte complexes; cumulus cells; gene ontology; ovary; sus scrofa; VEGFA

Abstract

V originále

Background: A frequent reason for in vitro fertilization failure is insufficient oocyte competence and difficulties in mimicking optimal in vivo conditions at the stage of in vitro maturation. This study aimed to identify the expression profile of genes associated with "vasculature development", "circulatory system process", "hemopoiesis", "patterning of blood vessels" and "hemopoietic or lymphoid organ development" ontological groups during in vitro maturation of domestic pig oocytes. The goal was also to describe new genetic markers that can be used in assisted reproduction techniques. Methods: Porcine oocytes were subjected to in vitro maturation, and the expression of selected genes was compared before and after maturation. Microarray and reverse transcription quantitative polymerase chain reaction (RT-qPCR) analysis were performed on total RNA isolated from the samples. Additionally, immunocytochemical staining was performed and the histological morphology of ovaries was examined. Results: Regardless of the individual processes, the gene expression pattern remained the same and all 32 gene ontology biological process term-genes were represented in down-regulated gene sets. The greatest change in expression was shown by the Vascular Endothelial Growth Factor A (VEGFA) and the smallest by myoferlin (MYOF). Moreover, the expression of VEGFA was confirmed by immunohistochemical analysis of ovaries. Conclusions: The potential contribution of chosen genes to oocyte performance during in vitro maturation can enhance comprehension of the underlying processes in oocytes and their microenvironment. It may also improve the effectiveness of in vitro fertilization.

Citovat

BOROWIEC, Blanka Maria, Wieslawa KRANC, Magdalena KULUS, Patrycja SUJKA-KORDOWSKA, Aneta KONWERSKA, Piotr CELICHOWSKI, Malgorzata JOZKOWIAK, Jakub KULUS, Malgorzata BLATKIEWICZ, Michal JEŠETA, Paul MOZDZIAK and Bartosz KEMPISTY. New Gene Markers Regulating the Process of Vascularization in Pig Preovulatory Oocytes during Growth and Maturation. Journal of biological regulators and homeostatic agents. Silva Marina: Biolife SAS, 2024, vol. 38, No 4, p. 2807-2824. ISSN 0393-974X. Available from: https://dx.doi.org/10.23812/j.biol.regul.homeost.agents.20243804.219.

@article{2407938,
   author = {Borowiec, Blanka Maria and Kranc, Wieslawa and Kulus, Magdalena and SujkaandKordowska, Patrycja and Konwerska, Aneta and Celichowski, Piotr and Jozkowiak, Malgorzata and Kulus, Jakub and Blatkiewicz, Malgorzata and Ješeta, Michal and Mozdziak, Paul and Kempisty, Bartosz},
   article_location = {Silva Marina},
   article_number = {4},
   doi = {http://dx.doi.org/10.23812/j.biol.regul.homeost.agents.20243804.219},
   keywords = {cumulus-oocyte complexes; cumulus cells; gene ontology; ovary; sus scrofa; VEGFA},
   language = {eng},
   issn = {0393-974X},
   journal = {Journal of biological regulators and homeostatic agents},
   title = {New Gene Markers Regulating the Process of Vascularization in Pig Preovulatory Oocytes during Growth and Maturation},
   url = {https://www.biolifesas.org/EN/10.23812/j.biol.regul.homeost.agents.20243804.219},
   volume = {38},
   year = {2024}
}

TY  - JOUR
ID  - 2407938
AU  - Borowiec, Blanka Maria - Kranc, Wieslawa - Kulus, Magdalena - Sujka-Kordowska, Patrycja - Konwerska, Aneta - Celichowski, Piotr - Jozkowiak, Malgorzata - Kulus, Jakub - Blatkiewicz, Malgorzata - Ješeta, Michal - Mozdziak, Paul - Kempisty, Bartosz
PY  - 2024
TI  - New Gene Markers Regulating the Process of Vascularization in Pig Preovulatory Oocytes during Growth and Maturation
JF  - Journal of biological regulators and homeostatic agents
VL  - 38
IS  - 4
SP  - 2807-2824
EP  - 2807-2824
PB  - Biolife SAS
SN  - 0393974X
KW  - cumulus-oocyte complexes
KW  - cumulus cells
KW  - gene ontology
KW  - ovary
KW  - sus scrofa
KW  - VEGFA
UR  - https://www.biolifesas.org/EN/10.23812/j.biol.regul.homeost.agents.20243804.219
N2  - Background: A frequent reason for in vitro fertilization failure is insufficient oocyte competence and difficulties in mimicking optimal in vivo conditions at the stage of in vitro maturation. This study aimed to identify the expression profile of genes associated with "vasculature development", "circulatory system process", "hemopoiesis", "patterning of blood vessels" and "hemopoietic or lymphoid organ development" ontological groups during in vitro maturation of domestic pig oocytes. The goal was also to describe new genetic markers that can be used in assisted reproduction techniques. Methods: Porcine oocytes were subjected to in vitro maturation, and the expression of selected genes was compared before and after maturation. Microarray and reverse transcription quantitative polymerase chain reaction (RT-qPCR) analysis were performed on total RNA isolated from the samples. Additionally, immunocytochemical staining was performed and the histological morphology of ovaries was examined. Results: Regardless of the individual processes, the gene expression pattern remained the same and all 32 gene ontology biological process term-genes were represented in down-regulated gene sets. The greatest change in expression was shown by the Vascular Endothelial Growth Factor A (VEGFA) and the smallest by myoferlin (MYOF). Moreover, the expression of VEGFA was confirmed by immunohistochemical analysis of ovaries. Conclusions: The potential contribution of chosen genes to oocyte performance during in vitro maturation can enhance comprehension of the underlying processes in oocytes and their microenvironment. It may also improve the effectiveness of in vitro fertilization.
ER  -

BOROWIEC, Blanka Maria, Wieslawa KRANC, Magdalena KULUS, Patrycja SUJKA-KORDOWSKA, Aneta KONWERSKA, Piotr CELICHOWSKI, Malgorzata JOZKOWIAK, Jakub KULUS, Malgorzata BLATKIEWICZ, Michal JEŠETA, Paul MOZDZIAK and Bartosz KEMPISTY. New Gene Markers Regulating the Process of Vascularization in Pig Preovulatory Oocytes during Growth and Maturation. \textit{Journal of biological regulators and homeostatic agents}. Silva Marina: Biolife SAS, 2024, vol.~38, No~4, p.~2807-2824. ISSN~0393-974X. Available from: https://dx.doi.org/10.23812/j.biol.regul.homeost.agents.20243804.219.

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