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@article{2409437, author = {Patakova, Petra and Branska, Barbora and Sedlar, Karel and Vasylkivska, Maryna and Jureckova, Katerina and Kolek, Jan and Koscova, Pavlina and Provaznik, Ivo}, article_location = {London}, article_number = {Feb}, doi = {http://dx.doi.org/10.1038/s41598-018-37679-0}, keywords = {ACETONE-BUTANOL FERMENTATION; CYCLOPROPANE FATTY-ACIDS; FACTOR HOMOLOGOUS GENES; BACILLUS-SUBTILIS; SOLVENT PRODUCTION; ESCHERICHIA-COLI; DIFFERENTIAL EXPRESSION; GROWTH TEMPERATURE; HISTIDINE KINASES; DIVISION SITE}, language = {eng}, issn = {2045-2322}, journal = {Nature Scientific Reports}, title = {Acidogenesis, solventogenesis, metabolic stress response and life cycle changes in <i>Clostridium beijerinckii</i> NRRL B-598 at the transcriptomic level}, url = {https://www.nature.com/articles/s41598-018-37679-0}, volume = {9}, year = {2019} }
TY - JOUR ID - 2409437 AU - Patakova, Petra - Branska, Barbora - Sedlar, Karel - Vasylkivska, Maryna - Jureckova, Katerina - Kolek, Jan - Koscova, Pavlina - Provaznik, Ivo PY - 2019 TI - Acidogenesis, solventogenesis, metabolic stress response and life cycle changes in <i>Clostridium beijerinckii</i> NRRL B-598 at the transcriptomic level JF - Nature Scientific Reports VL - 9 IS - Feb SP - 1-21 EP - 1-21 PB - NATURE RESEARCH SN - 20452322 KW - ACETONE-BUTANOL FERMENTATION KW - CYCLOPROPANE FATTY-ACIDS KW - FACTOR HOMOLOGOUS GENES KW - BACILLUS-SUBTILIS KW - SOLVENT PRODUCTION KW - ESCHERICHIA-COLI KW - DIFFERENTIAL EXPRESSION KW - GROWTH TEMPERATURE KW - HISTIDINE KINASES KW - DIVISION SITE UR - https://www.nature.com/articles/s41598-018-37679-0 N2 - Clostridium beijerinckii NRRL B-598 is a sporulating, butanol and hydrogen producing strain that utilizes carbohydrates by the acetone-butanol-ethanol (ABE) fermentative pathway. The pathway consists of two metabolic phases, acidogenesis and solventogenesis, from which the latter one can be coupled with sporulation. Thorough transcriptomic profiling during a complete life cycle and both metabolic phases completed with flow cytometry, microscopy and a metabolites analysis helped to find out key genes involved in particular cellular events. The description of genes/operons that are closely involved in metabolism or the cell cycle is a necessary condition for metabolic engineering of the strain and will be valuable for all C. beijerinckii strains and other Clostridial species. The study focused on glucose transport and catabolism, hydrogen formation, metabolic stress response, binary fission, motility/chemotaxis and sporulation, which resulted in the composition of the unique image reflecting clostridial population changes. Surprisingly, the main change in expression of individual genes was coupled with the sporulation start and not with the transition from acidogenic to solventogenic metabolism. As expected, solvents formation started at pH decrease and the accumulation of butyric and acetic acids in the cultivation medium. ER -
PATAKOVA, Petra, Barbora BRANSKA, Karel SEDLAR, Maryna VASYLKIVSKA, Katerina JURECKOVA, Jan KOLEK, Pavlina KOSCOVA and Ivo PROVAZNIK. Acidogenesis, solventogenesis, metabolic stress response and life cycle changes in \&{}lt;i\&{}gt;Clostridium beijerinckii\&{}lt;/i\&{}gt; NRRL B-598 at the transcriptomic level. \textit{Nature Scientific Reports}. London: NATURE RESEARCH, 2019, vol.~9, Feb, p.~1-21. ISSN~2045-2322. Available from: https://dx.doi.org/10.1038/s41598-018-37679-0.
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