J 2019

Acidogenesis, solventogenesis, metabolic stress response and life cycle changes in <i>Clostridium beijerinckii</i> NRRL B-598 at the transcriptomic level

PATAKOVA, Petra, Barbora BRANSKA, Karel SEDLAR, Maryna VASYLKIVSKA, Katerina JURECKOVA et. al.

Basic information

Original name

Acidogenesis, solventogenesis, metabolic stress response and life cycle changes in <i>Clostridium beijerinckii</i> NRRL B-598 at the transcriptomic level

Authors

PATAKOVA, Petra, Barbora BRANSKA, Karel SEDLAR, Maryna VASYLKIVSKA, Katerina JURECKOVA, Jan KOLEK, Pavlina KOSCOVA and Ivo PROVAZNIK

Edition

Nature Scientific Reports, London, NATURE RESEARCH, 2019, 2045-2322

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10700 1.7 Other natural sciences

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 3.998

DOI

UT WoS

000457616300259

Keywords in English

ACETONE-BUTANOL FERMENTATION; CYCLOPROPANE FATTY-ACIDS; FACTOR HOMOLOGOUS GENES; BACILLUS-SUBTILIS; SOLVENT PRODUCTION; ESCHERICHIA-COLI; DIFFERENTIAL EXPRESSION; GROWTH TEMPERATURE; HISTIDINE KINASES; DIVISION SITE

Tags

Tags

International impact, Reviewed
Změněno: 15/10/2024 09:07, Ing. Martina Blahová

Abstract

V originále

Clostridium beijerinckii NRRL B-598 is a sporulating, butanol and hydrogen producing strain that utilizes carbohydrates by the acetone-butanol-ethanol (ABE) fermentative pathway. The pathway consists of two metabolic phases, acidogenesis and solventogenesis, from which the latter one can be coupled with sporulation. Thorough transcriptomic profiling during a complete life cycle and both metabolic phases completed with flow cytometry, microscopy and a metabolites analysis helped to find out key genes involved in particular cellular events. The description of genes/operons that are closely involved in metabolism or the cell cycle is a necessary condition for metabolic engineering of the strain and will be valuable for all C. beijerinckii strains and other Clostridial species. The study focused on glucose transport and catabolism, hydrogen formation, metabolic stress response, binary fission, motility/chemotaxis and sporulation, which resulted in the composition of the unique image reflecting clostridial population changes. Surprisingly, the main change in expression of individual genes was coupled with the sporulation start and not with the transition from acidogenic to solventogenic metabolism. As expected, solvents formation started at pH decrease and the accumulation of butyric and acetic acids in the cultivation medium.